Binding of Short Dna Oligonucleides to the Surface of Reduced Graphene Oxide to Create Biological Sensors

Abstract

The study examined the possibility of immobilization of short DNA oligonucleotides (aptmers) on the surface of reduced graphene oxide, with the aim of forming a bio-sensitive sensory layer. The features of laser reduction of graphene oxide were considered, and the parameters of reduction were determined, which were implicated in achieving partial reduction. The possibility of immobilization of aptamers on graphene oxide with a low degree of reduction and the response of sensory structures formed on the basis of this basis is shown. A significant difference between the response of the sensors to the effects of thrombin (target protein) and albumin (comparison protein) was demonstrated.