Binding of stimulated human peripheral blood lymphocytes to Sepharose-concanavalin A is not reversed by methyl-α-mannoside

Although suppressor cells appear to be involved in the normal regulatory mechanism of the lymphoid system, they are also considered to have a role in the immunosuppression of certain malignancies. Suppressor activity of lymphocytes can be reproducibly measured by use of the mixed lymphocyte culture-mitogen interaction (MLC-M) in which the stimulating cell either is in the basal state or has been induced by Concanavalin A. This yields a quantitative measure of resting suppressor cell activity as well as the maximum generation of suppressor activity as induced by Con A. This test was performed, using autologous and allogenic cell combinations in a group of 13 cancer patients and 18 normal controls. Normal lymphocytes activated by Con A in 48-hour lymphocyte cultures significantly decreased the mitogenic response of lymphocytes from healthy, male donors to phytohemagglutinin (PHA), Con A, and Pokeweed mitogen (PWM) in both autologous and homologous systems. In contrast, Con A activated lymphocytes from cancer patients demonstrated diminished suppressor activity compared with controls in autologous (P less than 0.01), and allogenic (P less than 0.005) systems. There was a correlation between the degree of immunosuppression and suppressor cell activation: i.e., the patients most depressed generally had the lowest suppressor cell activation. Untreated lymphocytes from cancer patients also exerted suppressive effects on normal lymphocyte responses, suggesting an increased resting level of suppressor cells. These data suggest that in addition to having depressed cellular immune responses, cancer patients frequently have reduced capability to generate suppressor cell activity, which implies a generalized defect in this aspect of immune regulation.

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Defective suppressor cell activity in cancer patients: A defect in immune regulation

Hubbard

Wanebo

Fukuda

et al. 1981

Cancer

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Nylon-fiber affinity selection of red blood cells and tissue culture cells on the basis of cell surface determinants

Phillips¹,

Kabat²,

Miller³

1980

Experimental Cell Research

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Expression of protease and protease-inhibitory activity in human mononuclear leukocyte cultures

Hatcher¹,

Norin²

1982

Experimental Cell Research

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Binding of stimulated human peripheral blood lymphocytes to Sepharose-concanavalin A is not reversed by methyl-α-mannoside

Cited by 4 publications

References 11 publications

Defective suppressor cell activity in cancer patients: A defect in immune regulation

Defective suppressor cell activity in cancer patients: A defect in immune regulation

Nylon-fiber affinity selection of red blood cells and tissue culture cells on the basis of cell surface determinants

Expression of protease and protease-inhibitory activity in human mononuclear leukocyte cultures

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