Binding of α-lactalbumin to oleic acid monolayer and its relevance to formation of HAMLET-like complexes

Dopierała, Katarzyna; Krajewska, Martyna; Prochaska, Krystyna

doi:10.1016/j.idairyj.2018.08.017

Cited by 9 publications

(12 citation statements)

References 38 publications

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“…We observed the initial growth of A / A 0 for 5 and 10 mN/m, reaching the maximum after 3800–4000 s, and stabilization of the curves at the levels close to 1.2 and 1.0, respectively. This behavior is the result of the diffusion and adsorption of α-LA at the interface, as it was shown in our previous study . The stabilization step may be the effect of strong interactions between OA and BLA III, leading to the incorporation of BLA III molecules into the film.…”

Section: Resultssupporting

confidence: 69%

“…The nature of this interaction may be because of the hydrophobic forces and hydrogen bonds formed between the amino acid residues in BLA III and the carboxyl group in the fatty acid. The magnitude of the expansion induced by the apo form of α-LA (abbreviated here as BLA III) is larger than that in the case of earlier reported calcium-loaded holo -α-lactalbumin . This behavior can be explained by the enhanced sensitivity of BLA III to pH after the removal of Ca 2+ ion.…”

Section: Resultsmentioning

confidence: 67%

“…The magnitude of the expansion induced by the apo form of α-LA (abbreviated here as BLA III) is larger than that in the case of earlier reported calcium-loaded holo-α-lactalbumin. 26 This behavior can be explained by the enhanced sensitivity of BLA III to pH after the removal of Ca 2+ ion. However, the same concentration of BLA III at pH 2 caused a much more intensive expansion than that on the water subphase.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Several different approaches were shown to prepare HAMLET-like complexes, including chromatography, direct titration, alkaline method, heat treatment, and even simple mixing procedure. , In our previous studies, we reported on the possible HAMLET-like complex formation in vitro, in simulated gastric conditions at the air/water interface . The Langmuir monolayer technique was presented as an excellent approach to mimic the complex formation in human body.…”

Section: Introductionmentioning

confidence: 99%

“…24,25 In our previous studies, we reported on the possible HAMLET-like complex formation in vitro, in simulated gastric conditions at the air/water interface. 26 The Langmuir monolayer technique was presented as an excellent approach to mimic the complex formation in human body. Human α-LA was found to be able to adsorb in the monolayer of OA, unfold, and permanently bind fatty acid molecules.…”

Section: ■ Introductionmentioning

confidence: 99%

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Temperature, pH, and Molecular Packing Effects on the Penetration of Oleic Acid Monolayer by α-Lactalbumin

et al. 2019

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Recently, we reported on the interfacial behavior of mixed oleic acid (OA)-α-lactalbumin monolayer and its relevance in the formation of tumoricidal HAMLET (human α-lactalbumin made lethal to tumor cells)-like complex. This complex is probably formed in the gastrointestinal tract, but it has not been proved so far. The molecular base and the underlying physicochemical forces leading to such complexation remain to be known as well. There are also several other issues related with the complex stoichiometry that need to be fully explained. This study provides insight into the mechanism of temperature, pH, and physical state of monolayer-dependent binding of OA by the milk proteinapo-α-lactalbumin. Using the Langmuir and Langmuir–Blodgett approaches, we investigated the interactions between the OA monolayer and the apo-bovine α-lactalbumin (BLA III) at different pH, temperatures, and molecular packing. We found that the most favorable conditions for the formation of mixed OA–BLA III film are relevant to the gastric environment. The stabilization of OA–BLA III at the interface is associated with the conformational changes of protein in the presence of fatty acids induced by low pH and high temperature in the expanded monolayer. Our approach helps to understand the molecular mechanism of HAMLET/bovine α-lactalbumin made lethal to tumor cells formation in vivo.

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Section: Resultssupporting

confidence: 69%

Section: Resultsmentioning

confidence: 67%

Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

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Temperature, pH, and Molecular Packing Effects on the Penetration of Oleic Acid Monolayer by α-Lactalbumin

et al. 2019

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Tear Film Constituents and Medicines for Eyes Investigated as Langmuir Films

Torrent‐Burgués

2023

BioNanoSci.

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The tear film has an important role on the quality of the vision process and is of importance in the fields of ophthalmology and optometry. The lipid layer, the outermost part of the tear film, can be investigated with the Langmuir technique, as well as the interaction of components of the aqueous part of the tear film with those of the lipid layer. Dysfunctions in the stability and composition of the tear film are the causes of eye diseases, as the dry eye syndrome. For the treatment of this disease, the artificial tears are used, and one type of them are the lipid tears, which can be investigated with the Langmuir technique. This technique can also be used to investigate the influence on the lipid layer of other artificial tear components or medicines instilled in the eye.This review presents investigations where the Langmuir technique has been used in relation with the tear film. In particular, the surface properties of natural tears and of four commercial lipid-containing artificial tears have been reported and discussed in connection with their composition.

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Using BAMLET complex in a functional spreadable cheese elaborated with bovine colostrum

et al. 2021

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Binding of α-lactalbumin to oleic acid monolayer and its relevance to formation of HAMLET-like complexes

Cited by 9 publications

References 38 publications

Temperature, pH, and Molecular Packing Effects on the Penetration of Oleic Acid Monolayer by α-Lactalbumin

Temperature, pH, and Molecular Packing Effects on the Penetration of Oleic Acid Monolayer by α-Lactalbumin

Tear Film Constituents and Medicines for Eyes Investigated as Langmuir Films

Using BAMLET complex in a functional spreadable cheese elaborated with bovine colostrum

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