Binding sites of 14C-actinomycin in amphibian ovocytes and an autoradiography technique for the detection of cytoplasmic DNA

Brachet, Jean; Ficq, Adrienne

doi:10.1016/0014-4827(65)90437-4

Cited by 83 publications

(20 citation statements)

References 7 publications

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“…This technique has been shown to be a valid method for the rough evaluation of the number of free DNA sites: the fixation of labelled actinomycin by the nuclei is closely linked to the degree of repression in chromatin (Brachet and Ficq, 1965;Brachet, 1971).…”

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Nuclear binding of tritiated actinomycin in surface epithelial cells from normal stomach and atrophic gastritis.

Stoffels¹,

Preumont²,

Reuck³

1978

Gut

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(Schindler, 1965; Siurala etal., 1966;Walker et al., 1971). Numerous authors have investigated cell proliferation kinetics in normal and atrophic stomach mucosa. An increased proliferation of the epithelial cells in the latter has been described (Deschner and Lipkin, 1976), while in cases of intestinalisation of the gastric mucosa, which is found in over 90% of gastric cancers (Morson, 1959), the same authors have described an impairment of epithelial cell maturation.Earlier work has reported an increased nuclear fixation of tritiated actinomycin (3H-AM) in cancer cells as compared to the corresponding normal cells Pileri et al., 1972;Heenen et al., 1973). This technique has been shown to be a valid method for the rough evaluation of the number of free DNA sites: the fixation of labelled actinomycin by the nuclei is closely linked to the degree of repression in chromatin (Brachet and Ficq, 1965;Brachet, 1971).'Address for correspondence: Dr G. Stoffels, H6pital Universitaire Brugmann, 4, Place Van Gehuchten, 1020 Bruxelles.Received for publication 4 May 1978 In this study, we have compared the binding of 3H-AM to chromatin in epithelial cells in normal stomachs and in atrophic gastritis by autoradiographs. Significant increases of 3H-AM binding would suggest that these cells are in a depressed state. MethodsTwenty-five patients were studied; eight had a normal gastric mucosa and 17 had a atrophic gastritis as defined by the histological criteria described by Whitehead et al. (1972).The grades of gastritis were (1) superficial, and (2) atrophic with three grades: mild, moderate, and severe. Superficial gastritis is characterised by mononuclear and polymorphonuclear cell infiltration located in the surface epithelium and gastric pit region. In mild atrophic gastritis the infiltration occurs into all layers of lamina propria with a slight reduction of parietal and chief cells. Moderate and severe atrophic gastritis includes all the above signs, in addition to a greater reduction in parietal and chief cells, and the presence of intestinal metaplasia and regenerative nodules.It must be remembered that in this classification 'active atrophic gastritis' signifies the presence of 870 on 25 March 2019 by guest. Protected by copyright.

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Nuclear binding of tritiated actinomycin in surface epithelial cells from normal stomach and atrophic gastritis.

Stoffels¹,

Preumont²,

Reuck³

1978

Gut

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“…From investigations of sex steroid action in cellproliferation studies (Galand & Leroy, 1971; Martin & Finn, 1971), it appears that different uterine cell types react differently to the same hormonal stimulus. The present paper evaluates genetic regulation by sex steroid action by using methods which enable the chromatin function at the level of individual cells to be studied, namely 'staining' of fixed uterine sections with labelled actinomycin-D followed by radioautography (Brächet & Ficq, 1965) and cytophotometric study of Feulgen reaction kinetics (Agrell & Bergqvist, 1962) on isolated endometrial nuclei. With both methods, different hormonal treatments were found to induce variable patterns of response in rat endometrium epithelial and stromal nuclei.…”

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confidence: 99%

Increased Chromatin Acid Lability and Actinomycin-D Binding in Endometrial Cells Under the Action of Sex Steroids

Leroy¹,

Preumont²,

Galand³

et al. 1972

Journal of Endocrinology

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Modifications of chromatin activity, induced by sex steroids, were studied in rat endometrial tissues by the following semi-quantitative cytochemical methods: 'staining' of fixed uteri by [3H]actinomycin-D followed by radioautography and cytophotometric evaluation of Feulgen reaction kinetics in isolated endometrial nuclei. The following hormonal treatments were applied to groups of animals: oestradiol-17 \g=b\(10\g=m\g);progesterone (2 mg); progesterone (2 mg daily for 4 days) with oestradiol-17\g=b\(0\ m=.\05\g=m\g on the 4th day). With both cytochemical methods, different hormonal treatments were found to induce different patterns of response in endometrial cell types. In luminal epithelium and endometrial stroma, hormonal treatment increased the binding of actinomycin-D in a variable manner as judged from autoradiographic grain counts. Sex steroids also induced an overall increased DNA acid-lability as estimated by cytophotometric evaluation of Feulgen staining intensity. Hormonal treatments induced different hydrolysis curve patterns in both endometrial nuclear types.

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“…The method of Brachet and F icq [17] was used for the autoradiographic evalu ation of 3H-actinomycin binding by the cells of normal bone marrow (5 cases), CML (4 cases), blast crisis in chronic myeloid leukaemia (BC, 2 cases) and AML (2 cases). All cases were in a relatively steady state and no cytostatic treatment had been previously administered in the CML and AML forms.…”

Section: Methodsmentioning

confidence: 99%

Myeloid Cell Actinomycin Binding in Human Myeloid Leukaemia

Pileri¹,

Masera²,

Garbarino³

et al. 1974

Acta Haematol

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Decreased actinomycin D binding was observed in the marrow cells of some patients with chronic myeloid leukaemia. Blast uptake was still reduced in 2 cases of blast crisis and in acute myeloblastic leukaemia. However, the differentiating cells in blast crisis, i.e., those that had passed the blast stage, had exactly the same actinomycin D binding values as normal myeloid cells. After 0.5 mg/kg methotrexate, increased actinomycin D binding on the part of the entire leukaemic population was observed in one case of acute myeloblastic leukaemia. These findings suggest differences in chromatin behaviour and genetic activity in myeloid cells in the different stages of myeloid leukaemia.

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Binding sites of 14C-actinomycin in amphibian ovocytes and an autoradiography technique for the detection of cytoplasmic DNA

Cited by 83 publications

References 7 publications

Nuclear binding of tritiated actinomycin in surface epithelial cells from normal stomach and atrophic gastritis.

Nuclear binding of tritiated actinomycin in surface epithelial cells from normal stomach and atrophic gastritis.

Increased Chromatin Acid Lability and Actinomycin-D Binding in Endometrial Cells Under the Action of Sex Steroids

Myeloid Cell Actinomycin Binding in Human Myeloid Leukaemia

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