Binding Sites of miR-1273 Family on the mRNA of Target Genes

Иващенко, А. Т.; Berillo, Olga; Pyrkova, Anna; Niyazova, Raigul

doi:10.1155/2014/620530

Cited by 27 publications

(25 citation statements)

References 19 publications

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“…Location of binding site in the protein coding region facilitates its conservation in evolution, especially, if the corresponding oligopeptide plays an important role in the function of protein. Similar results were obtained earlier [26,27].…”

Section: Rssgrrggss----rheksx Pabsupporting

confidence: 92%

Characteristics of interaction of miRNA with mRNA of E2F transcription factors family genes

et al. 2017

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Search of binding sites of 6266 miRNA with mRNA of genes of E2F transcription factors family was implemented. The mRNA of E2F1 gene is associated with 13 miRNAs in 5′UTR, CDS and 3′UTR. The mRNA of E2F2 gene has binding sites for 10 miRNAs. The binding sites of miR-1-875-3p and miR-760-3р are conservative in mRNA of E2F2 gene of 18 mammalian species. The mRNA of E2F3 gene contains binding sites for one miRNA in the 5'UTR, for two miRNAs in the 3'UTR and for other seven miRNAs in the CDS. The miR

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Section: Rssgrrggss----rheksx Pabsupporting

confidence: 92%

Characteristics of interaction of miRNA with mRNA of E2F transcription factors family genes

et al. 2017

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“…The up-regulation of miR-1273g-3p and its involvement in promoting cell migration, proliferation, and invasion via CNR1 gene have also been described in both lung and colorectal cancer cell lines (35,36). Furthermore, several binding sites for miR-1273g-3p have been identified on mRNAs of several other genes (NOL9, PLCXD1, ZNF490, CYP20A1, GNL3L, PPM1K, RBMS2, SAR1B, SLC35E2, IRCQ, ZNF850, MDM4) by using the target gene prediction program (37).…”

Section: Discussionmentioning

confidence: 91%

Clinical Significance of Circulating miR-1273g-3p and miR-122-5p in Pancreatic Cancer

et al. 2020

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The burden of pancreatic cancer (PanC) requires innovation in the current diagnostic approach. This study aimed to uncover new circulating microRNAs (miRNAs) that would distinguish patients with PanC from healthy subjects (HS) compared with the cancer antigen 19-9 (CA 19-9), and predict patients' clinical phenotypes and outcomes. MiRNA expression profiles in plasma were investigated by using a two-stage process. In a discovery phase, miRNAs levels were analyzed using the GeneChip TM miRNA 4.0 Affymetrix assay in 10 pools of plasma samples from PanC patients and HS; in a validation phase, significantly altered miRNAs were re-tested in independent cohorts of cancer patients and controls by droplet digital PCR (ddPCR). The diagnostic performance of the resulting miRNAs was compared to CA 19-9 determinations, and the associations of miRNAs plasma levels with patients' clinical phenotypes and outcomes were also taken into account. Bioinformatics selection of miRNAs differentially expressed in plasma uncovered miR-18a-5p, miR-122-5p, miR-1273g-3p, and miR-6126 as candidate oncogenic miRNAs in PanC. The ddPCR technology confirmed the significant over-expression of miR-122-5p, miR-1273g-3p, and miR-6126 in PanC compared to HS, in line with the trend of the CA 19-9 levels. Plasma levels of miR-1273g-3p, in combination with CA 19-9, showed higher power in distinguishing PanC patients from HS compared to the CA 19-9 tested alone, with a gain in both sensitivity and negative predictive value indicating a low false-negative rate (SE = 90.2% and NPV = 92.3% vs. SE = 82.1% and NPV = 87.9%). None of the oncogenic miRNAs were able to distinguish between a neoplastic and a proliferative/inflammatory disease of the pancreas, and were not able to stratify subjects according to the clinical risk for the disease. The only valuable association in PanC patients was found between miR-1273g-3p and tumor stage, and increased miR-122-5p levels emerged as independent negative prognostic factor for PanC patients (HR = 1.58, 95% CI = 1.03-2.43, p = 0.037). Our data highlighted a role for circulating miR-1273g-3p and miR-122-5p as new diagnostic and prognostic biomarkers for PanC.

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“…miR‐1273g‐3p, a member of the miR‐1273 family, is a newly discovered miRNA in 2011 . Hou's study showed that miR‐1273g‐3p could promote the migration of lung cancer A549 cells by inhibiting the expression of the cannabinoid receptor type 1 (CNR1) .…”

Section: Discussionmentioning

confidence: 99%

miR‐1273g‐3p modulates activation and apoptosis of hepatic stellate cells by directly targeting PTEN in HCV‐related liver fibrosis

Niu

et al. 2016

FEBS Letters

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MicroRNA (miRNA) play a pivotal role in the development of liver fibrosis. However, the functions of miRNA in hepatitis C virus (HCV)‐related liver fibrosis remain unclear. In this study, we systematically analyzed the microarray data of the serum miRNA in patients with HCV‐induced hepatic fibrosis. Among 41 dysregulated miRNA, miR‐1273g‐3p was the most significantly upregulated miRNA and correlated with the stage of liver fibrosis. Overexpression of miR‐1273g‐3p could inhibit translation of PTEN, increase the expression of α‐SMA, Col1A1, and reduce apoptosis in HSCs. Hence, we conclude that miR‐1273g‐3p might affect the activation and apoptosis of HSCs by directly targeting PTEN in HCV‐related liver fibrosis.

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Binding Sites of miR-1273 Family on the mRNA of Target Genes

Cited by 27 publications

References 19 publications

Characteristics of interaction of miRNA with mRNA of E2F transcription factors family genes

Characteristics of interaction of miRNA with mRNA of E2F transcription factors family genes

Clinical Significance of Circulating miR-1273g-3p and miR-122-5p in Pancreatic Cancer

miR‐1273g‐3p modulates activation and apoptosis of hepatic stellate cells by directly targeting PTEN in HCV‐related liver fibrosis

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