Binding the gap between experiments, statistics, and method comparison: A tutorial for computing limits of detection and quantification in univariate calibration for complex samples

Gegenschatz, Sofía A.; Chiappini, Fabricio A.; Teglia, Carla M.; Peña, Arsenio Muñoz de la; Goicoechea, Héctor C.

doi:10.1016/j.aca.2021.339342

Cited by 29 publications

(11 citation statements)

References 29 publications

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“…A good linear relationship is established from 1 fM to 100 pM with the regression equation as follows y = 10.019 + 0.625 × ( n = 3 , R 2 = 0.996 ) in which y stands for the peak current and x is the logarithmic protein concentration. The LOD of this sensor is calculated to be 0.75 fM (signal-to-noise ratio = 3) . The analytical performances show superiority compared with recently developed assays (Table ).…”

Section: Resultsmentioning

confidence: 86%

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Electrochemical Aptasensing of SARS-CoV-2 Based on Triangular Prism DNA Nanostructures and Dumbbell Hybridization Chain Reaction

2022

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Development of convenient, accurate, and sensitive methods for rapid screening of severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2) infection is highly desired. In this study, we have developed a facile electrochemical aptasensor for the detection of the SARS-CoV-2 S1 protein amplified by dumbbell hybridization chain reaction (DHCR). A triangular prism DNA (TPDNA) nanostructure is first assembled and modified at the electrode interface. Due to the multiple thiol anchors, the immobilization is quite stable. The TPDNA nanostructure also provides an excellent scaffold for better molecular recognition efficiency on the top single-strand region (DHP0). The aptamer sequence toward the SARS-CoV-2 S1 protein is previously localized by partial hybridization with DHP0. In the presence of the target protein, the aptamer sequence is displaced and DHP0 is exposed. After further introduction of the fuel stands of DHCR, compressed DNA linear assembly occurs, and the product can be stacked on the TPDNA nanostructure for the enrichment of electrochemical species. This electrochemical method successfully detects the target protein in clinical samples, which provides a simple, robust, and accurate platform with great potential utility.

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Section: Resultsmentioning

confidence: 86%

“…The LOD of this sensor is calculated to be 0.75 fM (signal-to-noise ratio = 3). 35 The analytical performances show superiority compared with recently developed assays ( Table 1 ). Meanwhile, the detection time is acceptable.…”

Section: Resultsmentioning

confidence: 91%

Electrochemical Aptasensing of SARS-CoV-2 Based on Triangular Prism DNA Nanostructures and Dumbbell Hybridization Chain Reaction

2022

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“…The instrument quantification limit (IQL) of lactic acid (0.493 μg/L) was obtained based on the signal-to-noise ratio (S/N) of 10. The LOQ of lactic acid was calculated to be 23.4 ng/g, according to the IQL and the multiple dilution in the pretreatment . For the lactic acid standard and PLA spiked in the sediment, ideal recoveries of 93.8–102.1% can be obtained (Table ), suggesting that the system was sufficient for the depolymerization of trace PLA in environmental samples.…”

Section: Resultsmentioning

confidence: 99%

“…The LOQ of lactic acid was calculated to be 23.4 ng/g, according to the IQL and the multiple dilution in the pretreatment. 24 For the lactic acid standard and PLA spiked in the sediment, ideal recoveries of 93.8−102.1% can be obtained (Table 1), suggesting that the system was sufficient for the depolymerization of trace PLA in environmental samples. Therefore, the LOQ of PLA MPs was calculated to be 18.7 ng/g on the basis of eq and no lactic acid background above the LOQ was found in the procedural blanks.…”

Section: Chemicalsmentioning

confidence: 97%

An In Situ Depolymerization and Liquid Chromatography–Tandem Mass Spectrometry Method for Quantifying Polylactic Acid Microplastics in Environmental Samples

Wang

Peng

et al. 2022

Environ. Sci. Technol.

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Polylactic acid (PLA) is the most commonly used biodegradable plastic with rapid growth in recent years. This leads to predictable increased pollution of PLA microplastics (MPs) in the environment. However, quantification methods for the PLA MPs are still lacking. In this study, a method based on alkaliassisted thermal depolymerization and liquid chromatography− tandem mass spectrometry (LC−MS/MS) analysis was developed to quantify PLA MPs. After the background monomer compound was removed, PLA MPs were efficiently depolymerized to lactic acid and detected by LC−MS/MS with a limit of quantification of 18.7 ng/g. The ideal recovery of spiked PLA MPs of 93% was obtained, and the PLA MPs did not need to be separated or extracted in advance from the environmental samples. Using this method, PLA MPs were detected in all the sediment samples of a reservoir at a range of 53.5−491 ng/g dw, and the concentrations decreased with the sediment depth. In addition, after soaking in water at 95 °C for 30 min, approximately 12 μg of PLA MPs was released from a single teabag.

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“…A literature review showed a gap between calibration methodologies [38,45] and results quality [46,47], particularly in terms of uncertainty and validation according to recent regulatory guidelines [48]. Because no consensus has been reached in quantification methodologies for endogenous compound quantification, the calibration parameters, such as matrix or analyte nature, number and concentration of calibrators are usually selected arbitrarily for each bioanalytical scientist's preference, without considering their potential impact on the accuracy of regression [49].…”

Section: Introductionmentioning

confidence: 99%

From fundamentals in calibration to modern methodologies: A tutorial for small molecules quantification in liquid chromatography–mass spectrometry bioanalysis

Visconti

Bernard

Feinberg

et al. 2023

Analytica Chimica Acta

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Binding the gap between experiments, statistics, and method comparison: A tutorial for computing limits of detection and quantification in univariate calibration for complex samples

Cited by 29 publications

References 29 publications

Electrochemical Aptasensing of SARS-CoV-2 Based on Triangular Prism DNA Nanostructures and Dumbbell Hybridization Chain Reaction

Electrochemical Aptasensing of SARS-CoV-2 Based on Triangular Prism DNA Nanostructures and Dumbbell Hybridization Chain Reaction

An In Situ Depolymerization and Liquid Chromatography–Tandem Mass Spectrometry Method for Quantifying Polylactic Acid Microplastics in Environmental Samples

From fundamentals in calibration to modern methodologies: A tutorial for small molecules quantification in liquid chromatography–mass spectrometry bioanalysis

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