Bio-Chemical Analysis and FTIR-Spectral Studies of Artificially Removed Renal Stone Mineral Constituents

Kanchana, G.; Sundaramoorthi, P.; Jeyanthi, G. P.

doi:10.4236/jmmce.2009.82014

Cited by 19 publications

(15 citation statements)

References 2 publications

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“…Scanning electron microscopy–energy dispersive X-ray spectroscopy (SEM-EDX) and XRF methods are used to understand their chemical compositions. 23 To determine the elemental composition and molecular crystal structure of kidney stones, XRD and XRF analysis were performed at the Scientific and Technological Research Council of Turkey-Marmara Research Center (TUBITAK MAM).…”

Section: Xrd and Xrf Analysismentioning

confidence: 99%

Analysis and Classification of Heterogeneous Kidney Stones Using Laser-Induced Breakdown Spectroscopy (LIBS)

et al. 2012

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Kidney stones were analyzed using laser-induced breakdown spectroscopy (LIBS), utilizing a high resolution multi-channel charge-coupled device (CCD) spectrometer and a nanosecond-pulse Nd : YAG laser. The kidney stones were also characterized using X-ray diffraction (XRD) and X-ray fluorescence (XRF) techniques for comparative analysis. It was found that the ratio of hydrogen (H) to carbon (C) was an important indicator of organic compounds such as uric acid. Advantages of LIBS, especially with regards to amount of sample required and sample preparation as well as the ability to carry out elemental analysis and classification of kidney stones simultaneously, over other analytical techniques such as XRD and XRF are discussed. The common minor elements detected in the kidney stones include P, S, Si, Ti, and Zn. Principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA) of broadband LIBS spectra were employed for classifying different types of kidney stones. The results are beneficial in understanding kidney stone formation processes, which can lead to preventive therapeutic strategies and treatment methods for urological patients.

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Section: Xrd and Xrf Analysismentioning

confidence: 99%

Analysis and Classification of Heterogeneous Kidney Stones Using Laser-Induced Breakdown Spectroscopy (LIBS)

et al. 2012

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“…) and C-C stretching (800 cm -1 ) were absent (Maitz et al, 2002;Boonchom, Danvirutai, 2009;Kanchana, Sundaramoorthi, Jeyanthi, 2009). Phosphates were suggested by the strongest vibrational symmetric mode for the P-O bond of the (PO 4 3-) group at 1100 cm -1 ; the intense band centered at 960 cm…”

Section: Resultsmentioning

confidence: 99%

Hypotensive effect and endothelium-dependent vascular action of leaves of Alpinia purpurata (Vieill) K. Schum

Silva

Lima

Caliman

et al. 2014

Braz. J. Pharm. Sci.

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The aims of this study were to evaluate the chemical profile, vascular reactivity, and acute hypotensive effect (AHE) of the ethanolic extract of leaves of Alpinia purpurata (Vieill) K. Schum (EEAP). Its chemical profile was evaluated using HPLC-UV, ICP-OES, and colorimetric quantification of total flavonoids and polyphenols. The vascular reactivity of the extract was determined using the mesenteric bed isolated from WKY. AHE dose-response curves were obtained for both EEAP and inorganic material isolated from AP (IAP) in WKY and SHR animals. Cytotoxic and mutagenic safety levels were determined by the micronucleus test. Rutin-like flavonoids were quantified in the EEAP (1.8 ± 0.03%), and the total flavonoid and polyphenol ratios were 4.1 ± 1.8% and 5.1 ± 0.3%, respectively. We observed that the vasodilation action of EEAP was partially mediated by nitric oxide ( ). The EEAP and IAP showed an AHE in WKY and SHR animals. EEAP did not have cytotoxic effects or cause chromosomic alterations. The AHE shown by EEAP could result from its endothelium-dependent vascular action. Rutin-like flavonoids, among other polyphenols, could contribute to these biological activities, and the calcium present in EEAP could act in a synergistic way.Uniterms: Alpinia. Alpinia purpurata/phytochemistry. Alpinia purpurata/ethanolic extract/vascular reactivity. Alpinia purpurata/ethanolic extract/acute hypotensive effect. Flavonoids/quantification. Polyphenols/quantification.Os objetivos deste estudo foram avaliar o perfil químico de folhas de Alpinia purpurata K. Schum (AP), assim como a reatividade vascular e o efeito hipotensor agudo (EHA) do extrato etanólico de folhas de AP (EEAP). Avliou-se o perfil químico utilizando-se HPLC-UV, ICP-OES e quantificação colorimétrica de flavonoides e polifenóis totais. A reatividade vascular foi determinada utilizando leito mesentérico isolado de ratos WKY. Curvas dose-resposta do EEAP e do material inorgânico da AP (IAP) foram realizadas em animais SHR e WKY. Determinaram-se a segurança citotóxica e mutagênica pelo teste de micronúcleos. Flavonoides tipo rutina foram quantificados no EEAP (1,8±0,03%) e flavonoide total e polifenóis foram de 4,1±1,8% e 5,1±0,3%, respectivamente. Observou-se ação vasodilatadora do EEAP, mediada parcialmente pelo óxido nítrico (

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“…Hence, in addition to the grouping of 5 bands, the presence of COM was con rmed with the presence of absorption bands at all the above speci ed wave numbers for COM. The discrete formation of two bands at 780 and 517cm − 1 was important in differentiating COM from COD (Kanchana et al (2009). Presence of phosphorous group was detected by absorption at 1040 cm − 1 (Kanchana et al 2009), phosphorous groups usually shows an absorption range of 1000-1100 cm − 1 .…”

Section: Characterization Of Urinary Calculi By Fourier Transform Infrared Spectroscopy (Ft-ir)mentioning

confidence: 97%

“…The vibration motions of atoms in bonds (bond stetching/ contracting/ bond wagging) are measured by passing FT-IR through the powdered stone sample, which has been compressed in to a nearly transparent wafer with adequate quantity of potassium bromide. In the FT-IR region (4000-200cm − 1 ), characteristic attenuation of incident energy occurs depending on the ability of molecules in the compound to oscillate at certain wavelengths (Kanchana et al 2009). even in mixed stone samples by strong band at 1010 cm − 1 due to absorption of PO 4 group and presence of other bands at 2377, 1476, 1437, 872, 770 and 574 cm − 1 .…”

Section: Characterization Of Urinary Calculi By Fourier Transform Infrared Spectroscopy (Ft-ir)mentioning

confidence: 99%

Surgical Management of Obstructive Urolithiasis and Fourier Transform Infrared (FT-IR) Spectroscopic Identification of Uroliths in Dogs: A Report of Six Cases

Thajunnisa

Philip

Dileepkumar

et al. 2021

Preprint

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Six dogs with signs of obstructive urolithiasis presented to University Veterinary Hospitals Mannuthy and Kokkalai, Kerala Veterinary and Animal Sciences University over a period of two months formed subject for the present study. All the animals were having history of difficult urination with clinical signs like stranguria, pollakuria, anuria and/or haematuria. Haemato-biochemical investigations, blood gas analysis, urinalysis, survey radiographs and ultrasonography were performed to arrive at a conclusive diagnosis. Surgical interventions were done under general anaesthesia using either urethrotomy, cystotomy or a combination of both, depending upon the site of obstruction. Post-operative pH modulators, anti-spasmodics, antibiotics and analgesic therapy were employed depending upon the condition of patient and all the animals had an uneventful recovery. When stones retrieved through surgery were subjected to Fourier Transform Infrared Spectroscopy (FT-IR) to identify the chemical composition, three samples were identified as a combination of struvite, calcium oxalate dihydrate and calcium apatite crystals and three samples were a mixture of calcium oxalate monohydrate and calcium phosphate. Identification of specific calculi composition using spectroscopy was helpful in prevention of recurrence through management practices and therapeutic diet postoperative.

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Bio-Chemical Analysis and FTIR-Spectral Studies of Artificially Removed Renal Stone Mineral Constituents

Abstract: The kidneys are the vital organs of the human body. Its function is a peculiar one and

Cited by 19 publications

References 2 publications

Analysis and Classification of Heterogeneous Kidney Stones Using Laser-Induced Breakdown Spectroscopy (LIBS)

Analysis and Classification of Heterogeneous Kidney Stones Using Laser-Induced Breakdown Spectroscopy (LIBS)

Hypotensive effect and endothelium-dependent vascular action of leaves of Alpinia purpurata (Vieill) K. Schum

Surgical Management of Obstructive Urolithiasis and Fourier Transform Infrared (FT-IR) Spectroscopic Identification of Uroliths in Dogs: A Report of Six Cases

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