Bio controlled efficacy of Bacillus thuringiensis cry protein protection against tomato fruit borer Helicoverpa armigera in a laboratory environment

Chandrakasan, Gobinath; Ayala, Manuel Toledano; Trejo, Juan Fernando García; Marcus, Gabriel; Maruthupandy, Muthuchamy; Kanisha, C. Chenthis; Murugan, M.; Galil, Fahd Mohammed Abd Al; Wadaan, Muhammad A.

doi:10.1016/j.pmpp.2022.101827

Cited by 5 publications

(2 citation statements)

References 30 publications

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“…DNA extraction was carried out on the symbiont bacteria according to [ 65 ], with slight modifications. The small subunit (16s) rRNA was amplified with a PCR system, and the final products were separated by agarose gel and visualized using a transilluminator imaging system following a previous laboratory screening procedure [ 66 ]. Here, we used forward primer-(16s 20) 5′-AGA GTT TGA TCC TGG CTC-3′ and reverse primer-(16s 1390) 5′-GAC GGG CGG TGT GTA CAA-3′.…”

Section: Methodsmentioning

confidence: 99%

Preliminary Screening on Antibacterial Crude Secondary Metabolites Extracted from Bacterial Symbionts and Identification of Functional Bioactive Compounds by FTIR, HPLC and Gas Chromatography–Mass Spectrometry

Chandrakasan,

García-Trejo,

Feregrino-Pérez

et al. 2024

Molecules

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Secondary metabolites, bioactive compounds produced by living organisms, can unveil symbiotic relationships in nature. In this study, soilborne entomopathogenic nematodes associated with symbiotic bacteria (Xenorhabdus stockiae and Photorhabdus luminescens) were extracted from solvent supernatant containing secondary metabolites, demonstrating significant inhibitory effects against E. coli, S. aureus, B. subtilus, P. mirabilis, E. faecalis, and P. stutzeri. The characterization of these secondary metabolites by Fourier transforms infrared spectroscopy revealed amine groups of proteins, hydroxyl and carboxyl groups of polyphenols, hydroxyl groups of polysaccharides, and carboxyl groups of organic acids. Furthermore, the obtained crude extracts were analyzed by high-performance liquid chromatography for the basic identification of potential bioactive peptides. Gas chromatography–mass spectrometry analysis of ethyl acetate extracts from Xenorhabdus stockiae identified major compounds including nonanoic acid derivatives, proline, paromycin, octodecanal derivatives, trioxa-5-aza-1-silabicyclo, 4-octadecenal, methyl ester, oleic acid, and 1,2-benzenedicarboxylicacid. Additional extraction from Photorhabdus luminescens yielded functional compounds such as indole-3-acetic acid, phthalic acid, 1-tetradecanol, nemorosonol, 1-eicosanol, and unsaturated fatty acids. These findings support the potential development of novel natural antimicrobial agents for future pathogen suppression.

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Section: Methodsmentioning

confidence: 99%

Preliminary Screening on Antibacterial Crude Secondary Metabolites Extracted from Bacterial Symbionts and Identification of Functional Bioactive Compounds by FTIR, HPLC and Gas Chromatography–Mass Spectrometry

Chandrakasan,

García-Trejo,

Feregrino-Pérez

et al. 2024

Molecules

Self Cite

View full text Add to dashboard Cite

show abstract

“…DNA extraction was carried out on the symbiont bacteria with a slight modification [66]. The small subunit (16s) rRNA was amplified with a PCR system, and the final products were separated by agarose gel and visualized by a transilluminator imaging system followed by previous screening of laboratory procedure [67]. Here, we used forward primer -(16s 20) 5' AGA GTT TGA TCC TGG CTC -3' and reverse primer -(16s 1390) 5' GAC GGG CGG TGT GTA CAA -3'.…”

Section: S Rdna Sequencing and Phylogenetic Analysismentioning

confidence: 99%

Preliminary Screening on Antibacterial Crude Secondary Metabolites Extracted from Bacterial Symbionts and Identification of Functional Bioactive Compounds by FTIR and Gas Chromatography-Mass Spectrometry

CHANDRAKASA

2024

Preprint

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Secondary metabolites are bioactive compounds produced by living organisms that can reveal symbiotic relationships in nature. In the work described here, soil-born entomopathogenic-nematodes associated with symbiotic bacteria (Xenorhabdus stockiae and Photorhabdus luminescens) extracted from solvent supernatant containing secondary metabolites showed significant inhibitory action against Escherichia coli, Staphylococcus aureus, Enterococcus faceium, Proteus vulgaris, Bacillus cereus, Bacillus subtilis. These secondary metabolites, characterized by Fourier transform infrared spectroscopy were found to be amine groups of proteins; hydroxyl and carboxyl groups of polyphenols; hydroxyl groups of polysaccharides, and carboxyl groups of organic acids. The major compounds identified by Gas chromatography-mass spectrometry analysis of ethyl acetate extracts of Xenorhabdus stockiae revealed the existence of Nonanoic acid derivatives, Paromycin, Pyrrolidinone, Octodecanal derivatives, Trioxa-5-aza-1-silabicyclo, 4-Octadecenal, Methyl ester, Oleic Acid and 1,2-benzenedicarboxylicacid. Additional extraction from Photorhabdus luminescens was expressed functional compounds such as Indole-3-acetic acid, Piperidinol derivatives, Phthalic acid, 1-Tetradecanol, Nemorosonol, 1-eicosanol and unsaturated fatty acids. These outcomes support the development of new alternative natural antimicrobial agents for pathogen suppression in the future.

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Plant secondary metabolites as bioactive substance for the sustainable agriculture

Sengottayan¹

2022

Physiological and Molecular Plant Pathology

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Bio controlled efficacy of Bacillus thuringiensis cry protein protection against tomato fruit borer Helicoverpa armigera in a laboratory environment

Cited by 5 publications

References 30 publications

Preliminary Screening on Antibacterial Crude Secondary Metabolites Extracted from Bacterial Symbionts and Identification of Functional Bioactive Compounds by FTIR, HPLC and Gas Chromatography–Mass Spectrometry

Preliminary Screening on Antibacterial Crude Secondary Metabolites Extracted from Bacterial Symbionts and Identification of Functional Bioactive Compounds by FTIR, HPLC and Gas Chromatography–Mass Spectrometry

Preliminary Screening on Antibacterial Crude Secondary Metabolites Extracted from Bacterial Symbionts and Identification of Functional Bioactive Compounds by FTIR and Gas Chromatography-Mass Spectrometry

Plant secondary metabolites as bioactive substance for the sustainable agriculture

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