Bio-deposition of a calcium carbonate layer on degraded limestone by Bacillus species

Dick, Jan; Windt, Wim De; Graef, Bernard De; Saveyn, Hans; Meeren, Paul Van der; Belie, Nele De; Verstraete, W.

doi:10.1007/s10532-005-9006-x

Cited by 351 publications

(167 citation statements)

References 23 publications

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“…As a result of the pH increment, ammonium (NH 4 + ) starts to dissociate to ammonia (NH 3 at a pH of about 9.3. The produced carbonate ions precipitate in the presence of calcium ions and form calcium carbonate crystals, which form cementing bridges between the existing sand grains [8], [12]- [14]. In bacterial cells, such as S. pasteurii, they act themselves as nucleation sites, causing localized cementation at specific points that can be controlled; thus forming stronger bonds with the solid grain particles.…”

Section: A Biomineralization Conceptmentioning

confidence: 99%

Bacterial “Masons” at Work with Wastes for Producing Eco-cement

Cuzman¹,

Wittig²,

Abancéns³

et al. 2015

IJESD

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Abstract-Industrial waste is now a global concern, causing environmental and economic harm. Industries are rapidly trying to find a solution, searching for optimal ways to manage waste and to change the most common practices, as landfill or incineration. Industrial waste is a very heavy burden for the environment, where a significant proportion of this industrial waste is attributable to construction and demolition waste. To mitigate these threats, a novel biomimetic technology for enzyme-based microbial carbonate precipitation was tested, converting industrial wastes into an ecological product. Within the European Ecocement project (FP7-Grant 282922), a novel eco-cement product was obtained by recovering valuable resources from different industries such as the dairy industry, cement industry and poultry growing industry. The eco-cement product involves the microbial carbonate precipitation process, via urea hydrolysis, in the presence of Sporosarcina pasteurii, a common soil ureolytic bacterium. This paper presented the general concept of the project and the main obtained results.

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Section: A Biomineralization Conceptmentioning

confidence: 99%

Bacterial “Masons” at Work with Wastes for Producing Eco-cement

Cuzman¹,

Wittig²,

Abancéns³

et al. 2015

IJESD

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“…Some bacteria that produce minerals by BIM have been used to repair limestone monuments [13][14][15][16] and to fill pores and cracks in concrete and other cementitious materials [17][18][19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Crack filling in concrete by addition of Bacillus subtilis spores – Preliminary study

Schwantes-Cezario¹,

Peres²,

Fruet³

et al. 2018

DYNA

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This study aimed to analyze the performance of two concrete mixtures with the addition of B. subtilis spores regarding their capacity of filling cracks during the curing time, evaluating whether the addition can influence the compressive strength of the concrete. For so, six concrete mixtures were studied divided in two mix compositions: 1:1:2 and 1:2:3 (cement: sand: gravel) with w/c of 0.33 and 0.45, respectively. For each composition were added concentrations of 0, 0.3x10 8 or 1.2x10 8 of B. subtilis spores/mL. The results showed that all the samples with spores addition presented crystals precipitation, possibly calcium carbonate, that visually filled the cracks. However, more studies of microstructural analysis should be conducted to prove that the cracks were closed. The compressive strength presented satisfactory results, because the addition of 1.2x10 8 spores/mL did not present significant differences in compressive strength at probability level of 5%, when compared to the reference concrete.Keywords: bioconcrete; concrete with addition of spores; Bacillus subtilis; crack filling; cracks.Relleno de fisuras en concreto por la adición de esporas de Bacilos subtilis -Estudio preliminar ResumenEste estudio tiene como objetivo analizar el desempeño de dos composiciones de hormigón con adición de esporas de B. sutilis con relación a su capacidad de relleno de fisuras durante el periodo de cura, evaluando la influencia de la adición en la resistencia a compresión. Para ello han sido estudiadas seis composiciones de hormigón, divididas en dos dosificaciones: 1:1:2 y 1:2:3 (cemento: árido fino: grava) en peso, con relación a/c de 0,33 y 0,45. Para cada una de las mezclas se utilizaran concentraciones de 0, 0.3x10 8 or 1.2x10 8 de esporas de B. sutilis/mL. Los resultados obtenidos han demostrado que todas las muestras con adición de esporas presentaron precipitación de cristales, posiblemente carbonato de calcio, que fue visualizado en las fisuras. Más estudios microestructurales deberán ser realizados para comprobar que de hecho ocurrió el relleno de las fisuras. La resistencia a compresión presentó resultados satisfactorios, pues no hubo diferencias significativas en relación al hormigón de referencia, a nivel de 5% de probabilidad.Palabras clave: biohormigón; hormigón con adición de esporas; Bacilos subtilis; relleno de fisuras; fisuras.

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“…The lower the water-cement ratio, the higher is the concrete strength. Dick et al (2006) analysed the seven parameters involved in the microbial concrete process such as calcite deposition on limestone cubes, pH increase, urea degrading capacity, extracellular polymeric substances (EPS) production, biofilm formation, potential and deposition of dense crystal layers. The bacterial treatment in the cement mortar enhances the durability of cement mortar/concrete (Grabiec et al, 2012;Pacheco-Torgal and Labrincha, 2013).…”

Section: Introductionmentioning

confidence: 99%

Enrichment of compressive strength in microbial cement mortar

Senthilkumar¹,

Palanisamy²,

Vijayakumar³

2014

Advances in Cement Research

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The method of microbial mineral plugging in porous media is common in nature. Physical and biochemical properties of calcium carbonate (CaCO 3 ) precipitation induced by Enterobacter and Serratia microorganisms into cement mortar specimens are studied and analysed. X-ray diffraction is used to identify the calcium carbonate crystal as calcite, vaterite, aragonite. Scanning electron microscopy (SEM) is used to verify the formations of white precipitation (calcium carbonate) in the microbial cement mortars. The improvement of strength in the concrete/cement mortar base is attributable to the formation of calcium carbonate, which fills the pores between the cement sand matrices. In the present study a noteworthy enhancement of compressive strength of 44% is observed in the biocuring Enterobactertreated specimen relative to control. This method of implanting semi-solid mixtures onto the surface of the cement paste specimens shows significant effects such as decrease of permeability and capillary water penetration.

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Bio-deposition of a calcium carbonate layer on degraded limestone by Bacillus species

Cited by 351 publications

References 23 publications

Bacterial “Masons” at Work with Wastes for Producing Eco-cement

Bacterial “Masons” at Work with Wastes for Producing Eco-cement

Crack filling in concrete by addition of Bacillus subtilis spores – Preliminary study

Enrichment of compressive strength in microbial cement mortar

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