Bio-distribution of Carbon Nanoparticles Studied by Photoacoustic Measurements

Dubyk, Kateryna; Borisova, Tatiana; Paliienko, Konstantin; Krisanova, Natalia; Isaiev, Mykola; Алексеев, С. А.; Skryshevsky, V. A.; Lysenko, V. A.; Géloën, Alain

doi:10.1186/s11671-022-03768-3

Cited by 13 publications

(5 citation statements)

References 39 publications

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“…As a further outlook, we assume that the formation of composite C ND-based nanostructures can extend the application range of fluorescent carbon nanodots in the field of life science. As mentioned above, C NDs are employed in fluorescent and photoacoustic bioimaging [43][44][45]. However, by merging with plasmonic elements in controlled conditions, they can also be employed as optical sensors using surface enhancement of Raman scattering (SERS), infrared absorption (SEIRA) signals or as multi-modal contrast or antibacterial agents [56,57] (Figure 7a).…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, germanium nanostructures can be employed for cell imaging [38], memory devices [39] and many other applications [40]. Finally, carbon nanodots (C NDs), exhibiting strong fluorescence in the visible spectral range [41,42], are promising for photonic and life science applications, in particular for optical bioimaging, water cleaning technologies and bimodal fluorescence-photoacoustic imaging [43][44][45]. Nevertheless, their application areas are still significantly limited due to the absence of other features, such as plasmonic or magnetic properties requiring the development of novel nanomaterials with easily controlled performance.…”

Section: Introductionmentioning

confidence: 99%

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“Green” Fluorescent–Plasmonic Carbon-Based Nanocomposites with Controlled Performance for Mild Laser Hyperthermia

Ryabchikov,

Zaderko

2023

Photonics

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Fluorescent carbon nanodots are a promising nanomaterial for different applications in biophotonics, sensing and optical nanothermometry fields due to their strong fluorescence properties. However, their multi-modal applications are considerably limited, requiring the use of several nanoagents that could solve different tasks simultaneously. In this paper, we report the first experimental results on a facile “green” laser-based synthesis of multi-modal carbon–metallic nanocomposites with tuned optical performance. This simple approach leads to the appearance of finely controlled plasmonic properties in carbon-based nanocomposites whose spectral position is adapted by using an appropriate material. Thus, longer laser ablation provokes 29-fold increase in the absorption intensity of carbon–gold nanocomposites due to the increase in the metal content from 13% (30 s) to 53% (600 s). Despite strong plasmonic properties, the metal presence results in the quenching of the carbon nanostructures’ fluorescence (2.4-fold for C-Au NCs and 3.6-fold for C-Ag NCs for 600 s ablation time). Plasmonic nanocomposites with variable metal content reveal a ~3-fold increase in the laser-to-heat conversion efficiency of carbon nanodots matching the temperature range for mild hyperthermia applications. The findings presented demonstrate a facile approach to expanding the properties of chemically prepared semiconductor nanostructures due to the formation of novel semiconductor–metallic nanocomposites using a “green” approach. Together with the ease in control of their performance, it can considerably increase the impact of semiconductor nanomaterials in various photonic, plasmonic and biomedical applications.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“Green” Fluorescent–Plasmonic Carbon-Based Nanocomposites with Controlled Performance for Mild Laser Hyperthermia

Ryabchikov,

Zaderko

2023

Photonics

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“…Carbon dots used in this study were prepared by solvothermal carbonization of a mixture of urea and anhydrous citric acid, taken in a molar ratio of 2:1. CD preparation and characterization was described in detail in (Kuznietsova, et al, 2023b;Mussabek, et al, 2022;Ivanov, et al, 2021;Dubyk, et al, 2022). CD dressing was prepared by mixing CD solution in a saline (1 mg/mL) with commercially available hydrogel Hydrosorb Gel (Hartmann, Germany) that consisted of Ringer solution, carboxyethyl-and carboxymethyl-cellulose, in a 1:1 ratio.…”

Section: Methodsmentioning

confidence: 99%

Carbon dot dressing as a treatment of alkali-induced skin burns

Kuznietsova,

Ishchuk,

Bogatyryov

et al. 2024

Biol. Stud.

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Background. Chemical burns, comprising 5–10 % of total burns but causing 30 % of burn-related deaths, are now a notable concern in Ukraine. Current clinical protocols lack specific approaches for chemical burns, and research on this type of burn is limited. Carbon-based nanoparticles show promise for wound healing because of anti-inflammatory, antioxidant, and antibacterial activities. So, the ability of carbon dots obtained from citric acid and urea (further called CD) to improve the healing of alkali-induced skin burn was aimed to be discovered. Materials and Methods. The study was conducted on male Wistar rats. Burn was modeled by application of gauze disc soaked with 3 M NaOH solution on shaved skin of anesthetized rats for 10 min. A CD dressing, consisting of a CD solution (1 mg/mL) mixed with cellulose-based hydrogel that served as a vehicle, was applied to burned skin daily during a 7-day period. There were following groups: control (healthy rats), a burn-only group (rats that received no dressing), a burn + vehicle group (rats that received vehicle dressing), and a burn + CD group (rats that received CD dressing). The study involved monitoring of burn areas, conducting skin histopathology, and performing blood biochemical analyses. Results. The daily CD dressing significantly decreased alkali-induced burn area (by 76 % compared to 40 % in burn-only group) after seven daily dressings. The level of inflammation in the burn site was also less expressed in CD-treated animals, compared to respective controls (non-treated animals and animals treated with Vehicle). There was no substantial systemic toxicity of the burn (of such area) and its healing, manifested by absence of body weight loss, and absence of dramatical changes in serum biochemical parameters (indicators of liver and kidney function). However, animals of all the groups that experienced burns had a significantly lower body weight gain and mesenteric lymph nodes weight compared to healthy rats. Conclusions. So, the application of carbon dots mixed with hydrogel speeded up alkali-induced burn healing without negative impact on the organism.

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“…Resulted transparent dark-brown solution was centrifuged at 14,500 g for 10 min, and negligibly small precipitate was disposed. Detailed description of the synthesis and characterization, as well as fraction distribution, is provided at [ 12 ].…”

Section: Methodsmentioning

confidence: 99%

In vitro and in vivo toxicity of carbon dots with different chemical compositions

Kuznietsova,

Géloën,

Dziubenko

et al. 2023

Discover Nano

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Carbon dots (CDs) are easy-obtained nanoparticles with wide range of biological activity; however, their toxicity after prolonged exposure is poorly investigated. So, in vitro and in vivo toxicity of CDs with the surfaces enriched with hydroxylated hydrocarbon chains and methylene groups (CD_GE), carboxyl and phenol groups accompanied with nitrogen (CD_3011), trifluoromethyl (CDF19) or toluidine and aniline groups (CDN19) were aimed to be discovered. CDs’ in vitro toxicity was assessed on A549 cells (real-time cell analysis of impedance, fluorescence microscopy) after 24 h of incubation, and we observed no changes in cell viability and morphology. CDs’ in vivo toxicity was assessed on C57Bl6 mice after multiple dosages (5 mg/kg subcutaneously) for 14 days. Lethality (up to 50%) was observed in CDN19 and CD_3011 groups on different days of dosing, accompanied by toxicity signs in case of CD_3011. There were no changes in serum biochemical parameters except Urea (increased in CDF19 and CD_3011 groups), nor substantial kidney, liver, and spleen injuries. The most impactful for all organs were also CD_3011 and CDF19, causing renal tubule injury and liver blood supply violation. Thus, CDs with a surface enriched with oxygen- and nitrogen-containing functional groups might be toxic after multiple everyday dosing, without, however, significant damages of internal organs in survived animals.

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Bio-distribution of Carbon Nanoparticles Studied by Photoacoustic Measurements

Cited by 13 publications

References 39 publications

“Green” Fluorescent–Plasmonic Carbon-Based Nanocomposites with Controlled Performance for Mild Laser Hyperthermia

“Green” Fluorescent–Plasmonic Carbon-Based Nanocomposites with Controlled Performance for Mild Laser Hyperthermia

Carbon dot dressing as a treatment of alkali-induced skin burns

In vitro and in vivo toxicity of carbon dots with different chemical compositions

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