Bio-efficacy of tricyclazole 22.5% W/V + azoxystrobin 7.5% W/V against sheath blight disease of paddy

Pramesh, D.; Saddamhusen, A; Amoghavarsha, C; Sharanabasav, H; Raghunandana, A; Kumar, MH Mahesh; Maruti,; Nagaraj, B. T.; Raghavendra, B. T.; Huruli, Sujay; Reddy, B.G. Suryanaryana; Mahanthashivayogayya, K

doi:10.22271/chemi.2020.v8.i4ai.10095

Int. J. Chem. Stud.

2020

DOI: 10.22271/chemi.2020.v8.i4ai.10095

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Bio-efficacy of tricyclazole 22.5% W/V + azoxystrobin 7.5% W/V against sheath blight disease of paddy

D. Pramesh¹,

A Saddamhusen²,

C Amoghavarsha³

et al.

Abstract: A new combination fungicide Tricyclazole 22.5% W/V (20.36% W/W) + Azoxystrobin 7.5% W/V (6.79% W/W) was tested against rice sheath blight diseases under field condition during Kharif 2015 and Rabi 2015-16. The combination fungicide Tricyclazole 22.5% W/V (20.36% W/W) + Azoxystrobin 7.5% W/V (6.79% W/W) at 1000 ml/ha was found effective against sheath blight diseases by recording least Percent Disease Index (PDI) of 14.44 during Kharif 2015 and 13.35 during Rabi 2015-16. Significant increase in grain yield (61.… Show more

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Cited by 2 publications

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Thymol—a plant‐based monoterpenoid phenol compound of an essential oil for the management of sheath blight disease of rice

Usha,

Pramesh,

Shrikanth

et al. 2024

Pest Management Science

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Sheath blight of rice is a global disease that significantly reduces rice yield. This study reports the antifungal activity of an active compound of essential oil, thymol, at different concentrations against Rhizoctonia solani (strain RS‐Gvt). In vitro assay results indicated that thymol concentrations (0.5 mg mL−1 and 0.25 mg mL−1) completely inhibited (100%) the mycelial growth of RS‐Gvt (p ≤ 0.01). Microscopic observations of thymol‐treated mycelium of RS‐Gvt at 0.0312 mg mL−1 and above concentrations, revealed a distorted mycelial morphology with deformed hyphae. Hyphae showed a bead‐like appearance, reduction in size, and constriction of the hyphae at uneven points with increased hyphal density often entangling with each other. Further, an on‐field experiment was conducted to study the field bio‐efficacy of thymol for two consecutive Kharif seasons of 2022 and 2023 using a factorial RCBD design. The disease severity was measured as the percent disease index (PDI), and the results of two seasons were pooled. Pathogen (RS‐Gvt) and thymol were inoculated in different combinations/methods as main treatments (M1‐M3), and concentrations of thymol (0.0625–1.0 mg ML−1) as sub‐treatments. The results indicated that all two factors significantly (P = 0.05) influenced the PDI and grain yield. The pooled data of two seasons indicated a significant difference between the main treatments (M1: RS‐Gvt + thymol together; M2: thymol sprayed first followed by RS‐Gvt; M3: RS‐Gvt first followed by thymol spray) on PDI (53.39–59.67) and grain yield (4.16–4.75 t ha−1). M1 exhibited a lower PDI (53.39) and a higher grain yield (4.75 t ha−1) compared to M2 and M3, indicating a protective mode of action of thymol against sheath blight disease of rice. The sub‐treatments have shown significant variation in PDI and grain yield. The PDI and grain yield ranged from 33.70 (at 1 mg mL−1) to 66.21 (at 0.0625 mg mL−1) and 4.18 (at 1 mg mL−1) to 5.26 (at 0.0625 mg mL−1) t ha−1, respectively, among the thymol concentrations. This indicates that increasing concentrations of thymol have negatively influenced the PDI and positively impacted the yield. Therefore, the spray of 1 mg mL−1 of thymol at the potential disease‐infection stage is most effective in controlling the sheath blight disease of rice. This study provides an alternative green bioactive compound for controlling the sheath blight disease, and thymol can be included in developing eco‐friendly integrated disease management practices. © 2024 Society of Chemical Industry.

show abstract

Thymol—a plant‐based monoterpenoid phenol compound of an essential oil for the management of sheath blight disease of rice

Usha,

Pramesh,

Shrikanth

et al. 2024

Pest Management Science

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A simplified spore-drop technique for rapid isolation of rice blast pathogen Magnaporthe oryzae from the infected rice leaf

Amoghavarsha

Pramesh

Nagaraj

et al. 2022

Oryza

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Single spore isolation from a diseased sample is an essential step in obtaining a pure culture of a fungal pathogen. Rice blast disease caused by Magnaporthe oryzae is an inferior saprophytic competitor, and therefore, many fast-growing fungal or bacterial contaminants are predominant during its isolation. For isolation of M. oryzae, several methods are being followed; however, they are complex and often lead to contamination. In the present study, we have standardized an efficient method for rapid isolation of M. oryzae from the blast disease infected rice-leaf using single spore isolation by spore-drop technique. Following the spore-drop technique, pure culture for an isolate of M. oryzae was obtained quickly with the least contamination (4%), whereas the conventional spore-dilution and leaf-press method recorded 26.12 and 45.50% contamination, respectively. The spore-drop approach has yielded the single spore isolates in the shortest time (10 days) and can be used for regular rice blast pathogen isolation. This method can also be used for other sporulating pathogens successfully.

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Bio-efficacy of tricyclazole 22.5% W/V + azoxystrobin 7.5% W/V against sheath blight disease of paddy

Cited by 2 publications

References 10 publications

Thymol—a plant‐based monoterpenoid phenol compound of an essential oil for the management of sheath blight disease of rice

Thymol—a plant‐based monoterpenoid phenol compound of an essential oil for the management of sheath blight disease of rice

A simplified spore-drop technique for rapid isolation of rice blast pathogen Magnaporthe oryzae from the infected rice leaf

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