Bio-electrochemical microelectrode arrays for glutamate and electrophysiology detection in hippocampus of temporal lobe epileptic rats

Li, Ziyue; Song, Yilin; Xiao, Guihua; Gao, Fei; Xu, Shengwei; Wang, Mixia; Zhang, Yu; Guo, Fengru; Liu, Jie; Yang, Xiaobo; Cai, Xinxia

doi:10.1016/j.ab.2018.04.023

Cited by 34 publications

(22 citation statements)

References 33 publications

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“…The algorithms are developed based on the changes in motion trajectories, with respect to time, space, velocity, angular speed, and other factors. The basic principles are primarily taken from electroencephalogram, accelerometer, electromyograph, gyroscopes, magnetometer, and electrochemistry, which analyses the changes in acceleration, velocity, directions, ionic current, and electrical activity of the brain in the three-dimensional system [67,68].…”

Section: Nano-bio-sensing Regime Remediationmentioning

confidence: 99%

“…Pooling sensor-based data clues for a long time can help in forecasting seizures for specific individuals and particular triggers behind them. Nanobiosensors enable quantification of biomarkers such as dopamine (DoP), uric acid, ascorbic acid, hypoxanthine, acetylcholine, glutamate, lactate, and glucose which can be useful to correlate the neurochemical secretions with epilepsy [68,71,72,73].…”

Section: Nano-bio-sensing Regime Remediationmentioning

confidence: 99%

“…Biocompatible implantable MEA have shown promising results in acquiring data, monitoring the activity in the brain due to remarkable electrical sensitivity, discharge pattern of neurons, their synchronization, interictal spikes, local potential and concentration of biomarkers. Silicon-based MEA modified by platinum nanoparticles can perform a dual work of neural electrophysiology as well as quantifying neurotransmitter indicating epilepsy [68]. However, sometimes limited to the type of epilepsy under investigation, photoacoustic Imaging, utilizing the surface plasmon phenomenon of carbon-based materials or metallic/metallic oxide nanoparticles (CNT, Au and silver nanoparticles) and magnetic nanoparticles.…”

Section: State Of the Art Epilepsy Bio-sensing Techniquesmentioning

confidence: 99%

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Biosensors for Epilepsy Management: State-of-Art and Future Aspects

Tiwari

Sharma

Mujawar

et al. 2019

Sensors

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Epilepsy is a serious neurological disorder which affects every aspect of patients’ life, including added socio-economic burden. Unfortunately, only a few suppressive medicines are available, and a complete cure for the disease has not been found yet. Excluding the effectiveness of available therapies, the timely detection and monitoring of epilepsy are of utmost priority for early remediation and prevention. Inability to detect underlying epileptic signatures at early stage causes serious damage to the central nervous system (CNS) and irreversible detrimental variations in the organ system. Therefore, development of a multi-task solving novel smart biosensing systems is urgently required. The present review highlights advancements in state-of-art biosensing technology investigated for epilepsy diseases diagnostics and progression monitoring or both together. State of art epilepsy biosensors are composed of nano-enabled smart sensing platform integrated with micro/electronics and display. These diagnostics systems provide bio-information needed to understand disease progression and therapy optimization timely. The associated challenges related to the development of an efficient epilepsy biosensor and vision considering future prospects are also discussed in this report. This review will serve as a guide platform to scholars for understanding and planning of future research aiming to develop a smart bio-sensing system to detect and monitor epilepsy for point-of-care (PoC) applications.

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Section: Nano-bio-sensing Regime Remediationmentioning

confidence: 99%

Section: Nano-bio-sensing Regime Remediationmentioning

confidence: 99%

Section: State Of the Art Epilepsy Bio-sensing Techniquesmentioning

confidence: 99%

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Biosensors for Epilepsy Management: State-of-Art and Future Aspects

Tiwari

Sharma

Mujawar

et al. 2019

Sensors

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“…This release signal is tightly regulated by presynaptic and postsynaptic receptors and GLU transporters. The abnormally functioning GLU system causes the occurrence of nervous system diseases . Studies have reported that GLU and its receptors play an important role in the development of epilepsy.…”

Section: Discussionmentioning

confidence: 99%

SLC1A2 mediates refractory temporal lobe epilepsy with an initial precipitating injury by targeting the glutamatergic synapse pathway

et al. 2018

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This study aimed to identify the genes related to epilepsy and their effects on epilepsy, as well as the underlying mechanism. Using microarray analysis, differentially expressed genes (DEGs) were screened out and then used to build weighted gene coexpression networks using WGCNA. Module membership and evaluation of gene significance (GS) were adopted to detect hub genes. The DAVID online tool was used to understand the function of modules and target genes. The Licl‐pilocarpine chronic rat epilepsy model was used to simulate mesial temporal lobe epilepsy with an initial precipitating injury. Hippocampal expression of the proteins solute carrier family 1 member 2 (SLC1A2), glial fibrillary acidic protein, interleukin‐1β (IL‐1β), tumor necrosis factor α (TNF‐α), and N‐methyl‐d‐aspartic acid receptor (NMDAR) was determined by ELISA and Western blot. Nissl staining was used to measure neuronal loss. Immunohistochemistry was performed to assess the percentage of positive cells to reflect the distribution of NMDAR1. Here, 3232 potential genes highly correlated with epilepsy were selected from the screened DEGs, among which SLC1A2 was related to brain development and its expression was significantly decreased in epilepsy patients. According to Gene Ontology and KEGG analysis, SLC1A2 mediates epilepsy through the glutamatergic synapse pathway. Tissue experiments suggested that Slc1a2 could genuinely ameliorate epilepsy through the glutamatergic synapse pathway, mitigate neuronal loss, and suppress astrocytosis and inflammatory responses. Our study suggested that low hippocampal content of SLC1A2 is a potential biomarker of epilepsy and may affect the function of neurons through the glutamatergic synapse pathway. © 2018 IUBMB Life, 71(1):213–222, 2019

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“…Epilepsy is a common neurological disorder, affecting about 50 million people worldwide [1,2]. When the excitatory and inhibitory activities are out of balance, the abnormal discharges of neurons cause paroxysmal brain dysfunction, manifested as limb twitching or abnormal conscious behavior [3]. Glutamate (Glu) is a major excitatory neurotransmitter in the central nervous system, which has been related to the onset and propagation of seizures [4,5].…”

Section: Introductionmentioning

confidence: 99%

Flexible Electrocorticography Electrode Array for Epileptiform Electrical Activity Recording under Glutamate and GABA Modulation on the Primary Somatosensory Cortex of Rats

Song

Xiao

et al. 2020

Micromachines

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Epilepsy is a common neurological disorder. There is still a lack of methods to accurately detect cortical activity and locate lesions. In this work, a flexible electrocorticography (ECoG) electrode array based on polydimethylsiloxane (PDMS)-parylene was fabricated to detect epileptiform activity under glutamate (Glu) and gamma-aminobutyric acid (GABA) modulation on primary somatosensory cortex of rats. The electrode with a thickness of 20 μm has good flexibility to establish reliable contact with the cortex. Fourteen recording sites with a diameter of 60 μm are modified by electroplating platinum black nanoparticles, which effectively improve the performance with lower impedance, obtaining a sensitive sensing interface. The electrode enables real-time capturing changes in neural activity under drug modulation. Under Glu modulation, neuronal populations showed abnormal excitability, manifested as hypsarrhythmia rhythm and continuous or periodic spike wave epileptiform activity, with power increasing significantly. Under GABA modulation, the excitement was inhibited, with amplitude and power reduced to normal. The flexible ECoG electrode array could monitor cortical activity, providing us with an effective tool for further studying epilepsy and locating lesions.

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Bio-electrochemical microelectrode arrays for glutamate and electrophysiology detection in hippocampus of temporal lobe epileptic rats

Cited by 34 publications

References 33 publications

Biosensors for Epilepsy Management: State-of-Art and Future Aspects

Biosensors for Epilepsy Management: State-of-Art and Future Aspects

SLC1A2 mediates refractory temporal lobe epilepsy with an initial precipitating injury by targeting the glutamatergic synapse pathway

Flexible Electrocorticography Electrode Array for Epileptiform Electrical Activity Recording under Glutamate and GABA Modulation on the Primary Somatosensory Cortex of Rats

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