Bio-enhanced removal of hydrocarbon contents from spent engine oil contaminated soil using Staphylococcus aureus and Bacillus cereus co-culture

Abstract


 
 
 The study assessed the removal of total petroleum hydrocarbon (TPH) and polyaromatic hydrocarbons (PAHs) from spent engine oil (SEO) contaminated soil through bioenhancement of bacteria isolated from SEO polluted soil. Sterilized soil was subjected to a three level of SEO contamination before the addition of sterilized biostimulants including powdered cow dung (CD), powdered cocoa pod husk (CPH) and compost (made from fresh CPH and CD). Bacterial inoculum being Staphylococcus aureus and Bacillus cereus co-culture (150 mL) was added to the mixture in polyethylene bags. It was a factorial experiment that was laid out in a completely randomized design (CRD). The TPH and PAHs were estimated in the first day, fifth week and the tenth week that the room incubation lasted. Results generated from the influence of biostimulants on TPH and PAHs degradation potential of the bacterial co-culture showed that degradation of the hydrocarbon contents was significantly enhanced (p < 0.05). At the tenth week, compost enhanced the most TPH reductions (315 and 380 mg kg–1) compared with other biostimulants on 5% and 15% SEO contamination levels, respectively. Compost equally enhanced the most PAHs reductions (48.8, 39.6 and 94.6 mg kg–1) compared with other biostimulants on 5%, 10% and 15% SEO contamination levels respectively. However, the quantity of SEO contents degraded was significantly higher in the bioaugmented and biostimulated soil samples compared with the control employed. The technology adopted in this study can be effectively employed for the bioremediation of petroleum hydrocarbon related pollution.
 
 

Effect of mycorrhizal inoculation and organic fertiliser on bioremediation of spent engine oil contaminated soil

“…The results obtained from this study show enhanced TPH degradation due to compost application to the SEO-contaminated soil. The TPH reduction orchestrated through the bio-stimulatory influence of compost in this study corroborates previous findings by Agbor et al (2012); Agbor et al (2015); Adeleye et al (2020); Adeleye et al (2021), with reference to its capacity for such achievement. This further corroborates the findings of Okoh (2006), who reported that organic wastes bind rapidly to the soil particle, and this facilitates the movement of the pollutants through the soil when natural events like rain occur.…”

“…The addition of compost in this study resulted in enhanced TPH degradation in the SEO-contaminated soil. A similar result was also obtained by Adeleye et al (2021), where they observed a reduction in TPH and Polycyclic Aromatic Hydrocarbons (PAHs) content of SEO contaminated soil. The reduction in TPH content in this study could be due to the fact that mycorrhizal fungi notable active bio-fertilization interacted with some other beneficial soil organisms in order to achieve complete clean-up of polluted soils (Chibuike, 2013).…”

“…The experimental soil was randomly collected from the Teaching and Research Farm, Federal University Dutse at the depth of 0-15 cm using a shovel. Subsequently, it was exposed to air so as to form a compound sample as done by Adeleye et al (2021). The bulked soil was later sieved using a 2.0-mm mesh size (Soretire et al, 2017).…”

Potential of Mycorrhizal Inoculation and Cattle Rumen Digesta in the Bioremediation of Spent Engine Oil Contaminated Soil

“…Spent engine oil (SEO) is typically referred to as used motor oil that has been collected from mechanical workshops, garages, and industrial sources such as hydraulic oil, turbine oils, process oil, and metalworking fluids (Olufemi and Oladeji, 2008). Disposal of SEO into gutters, watercourses, open vacant plots, and farmland are common practice among auto machine operators (Adeleye et al, 2020). This practice practically increases the incidence of oil pollution of agricultural soils and the built environment.…”

Bacterias gram negativas, resistentes al cloro y degradantes de gasolina y diésel, aisladas de aguas subterráneas y costeras

“…A pesar de que los océanos no son afectados directamente por la eutrofización, debido a que las aguas profundas y frías, ricas en nutrientes, se encuentran por debajo de una capa establemente cálida, la cual constantemente pierde sustancias nutritivas, debido a que el plancton y el excremento del mismo se hunden (GIL et al, 2012;GONZÁLEZ CÁZAREZ et al, 2020). A través de la biorremediación, donde las cepas bacterianas, entre las que destacan las gram negativas, poseen características metabólicas que crecen en ambientes con altas concentraciones de metales o hidrocarburos (BHATNAGAR & KUMARI, 2013;ADELEYE et al, 2021), donde la relación intra e interpoblacional puede generar compuestos intermediarios que, a su vez, puede ser utilizado por otros microorganismos (NARVÁEZ FLÓREZ, GÓMEZ, Y MARTÍNEZ, 2008).…”