Bio-ethanol from water hyacinth biomass: An evaluation of enzymatic saccharification strategy

Aswathy, U.S.; Sukumaran, Rajeev K.; Devi, G. Lalitha; Rajasree, Kuniparambil; Singhania, Reeta Rani; Pandey, Ashok

doi:10.1016/j.biortech.2009.08.019

Cited by 136 publications

(61 citation statements)

References 18 publications

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“…The graph showed that, with the passage of time, the amount of reducing sugars increased and after 48.0 h there was an insignificant increase in the amount of sugars observed in some experiments. During hydrolysis, in the first 24.0 h more sugars were obtained and then sluggishly increased and reached a maximum at 48.0 h. Previously 71.3% enzymatic saccharification efficiency was reported by Aswathy et al, (2010) with NaOH pretreated water hyacinth and 60.2% by Mishima et al, (2008).…”

Section: Time Course Of Enzymatic Hydrolysismentioning

confidence: 95%

“…The plant body contains 26.3 wt% C-6 sugars such as glucose (19.8%) and galactose (6.5%) and 20.5 wt% C-5 sugars with 11.5 wt% xylose and arabinose (Girisuta et al, 2008;Aswathy et al, 2010). The pretreatment conditions had a significant influence on the amount of sugars released during pretreatment step and enzymatic hydrolysis.…”

Section: Effect Of Acid Concentration On Hydrolysismentioning

confidence: 99%

“…Among them, NaOH pretreatment could provide higher enzymatic saccharification as compared to acids (Zhao et al, 2007;Aswathy et al, 2010). During acidic or basic pretreatment, there occurred a loss in the biomass weight (Jiele et al, 2010;Wang et al, 2009) due to hydrolysis of hemicellulose and removal of lignin (Blasi et al, 1999;Lin et al, 2010).…”

Section: Most Effective Pretreatment Conditionmentioning

confidence: 99%

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Production of fermentable sugars by combined chemo-enzymatic hydrolysis of cellulosic material for bioethanol production

Muhammad

Adnan

Bokhari

et al. 2014

Braz. J. Chem. Eng.

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-To change the recalcitrant nature of the lignocellulosic material for maximum hydrolysis yield, a comprehensive study was done by using sulphuric acid as an exclusive catalyst for the pretreatment process. The enzymatic digestibility of the biomass [Water Hyacinth: Eichhornia crassipes] after pretreatment was determined by measuring the hydrolysis yield of the pretreated material obtained from twenty four different pretreatment conditions. These included different concentrations of sulphuric acid (0.0, 1.0, 2.0 and 3.0%), at two different temperatures (108 and 121 °C) for different residence times (1.0, 2.0 and 3.0h).The highest reducing sugar yield (36.65 g/L) from enzymatic hydrolysis was obtained when plant material was pretreated at 121 °C for 1.0 h residence time using 3.0% (v/v) sulphuric acid and at 1:10 (w/v) solid to liquid ratio. The total reducing sugars obtained from the two-stage process (pretreatment + enzymatic hydrolysis) was 69.6g/L. The resulting sugars were fermented into ethanol by using Saccharomyces cerevisiae. The ethanol yield from the enzymatic hydrolyzate was 95.2% of the theoretical yield (0.51g/g glucose), as determined by GS-MS, and nearly 100% since no reducing sugars were detected in the fermenting media by TLC and DNS analysis.

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Section: Time Course Of Enzymatic Hydrolysismentioning

confidence: 95%

Section: Effect Of Acid Concentration On Hydrolysismentioning

confidence: 99%

Section: Most Effective Pretreatment Conditionmentioning

confidence: 99%

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Production of fermentable sugars by combined chemo-enzymatic hydrolysis of cellulosic material for bioethanol production

Muhammad

Adnan

Bokhari

et al. 2014

Braz. J. Chem. Eng.

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“…Direct conversion of raw substrate to H 2 is difficult due to complex nature of lignin hemicellulose-cellulose complex; hence, various pre-treatment methods were carried out to break down the complex structure into simpler fragments so as to enable the bacterial action (Singhal and Singh 2014). Acidic and alkaline pre-treatment helps in the breakdown of cellulose into simpler monomer units of glucose (Sun and Cheng 2002;Li and Fang 2007;Aswathy et al 2009). Glucose is the carbon source on which microbial communities act upon to produce hydrogen through fermentation (Benemann 1996;Das and Veziroglu 2001;Levin et al 2006;Guo et al 2010).…”

Section: Hydrogen Production: Effect Of Acid Pretreatmentmentioning

confidence: 99%

“…The dry plant biomass mainly comprises of cellulose (18-31%), hemicellulose (18-43%) and lignin (7-26%) Bergier et al 2012;Barua and Kalamdhad 2016). The high content of carbohydrate can be hydrolysed through acidic and alkaline treatment into fermentable sugars Aswathy et al 2009;Barua and Kalamdhad 2016). Efforts done to tap the biomass as a suitable feedstock for the production of biofuels like biogas (Vivekanand et al 2013;Barua and Kalamdhad 2016), bioethanol (Das et al 2016;Shanab et al 2017), biohydrogen Chuang et al 2011;Lazaro et al 2014) and biodiesel (Shanab et al 2017) have been proved successful O'Sullivan et al 2010;Sharma et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Biohydrogen production by locally isolated facultative bacterial species using the biomass of Eichhornia crassipes: effect of acid and alkali treatment

Mechery¹,

Biji²,

Thomas³

et al. 2017

Energ. Ecol. Environ.

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Hydrogen (H 2 ) produced from biological methods is a potential option to meet the growing clean energy needs. The present study aimed to produce biohydrogen by dark fermentation from nuisance aquatic weed, Eichhornia crassipes, using facultative anaerobic bacteria. A total of 12 bacterial strains were isolated from different wastewater sources and were screened for the potential of H 2 production using glucose as carbon source. Ten strains showed the H 2 -producing potential and were identified up to the generic level by biochemical tests. Two strains with higher H 2 production were sequenced using PCR technique and identified as Proteus mirabilis and Pseudomonas aeruginosa and selected for the studies with E. crassipes as the substrate. It was found that P. aeruginosa could produce 19.54 ± 0.03% of H 2 from 2% acid (H 2 SO 4 ) treated substrate which was comparatively higher than that of 4 and 8% treatments. P. mirabilis also yielded better results of 5.42 ± 0.02% H 2 f or 2% acid (H 2 SO 4 ) treated substrate than 4 and 8% treatments. In total, 33.52 ± 0.04% of H 2 was produced by P. aeruginosa for the substrate treated with 2% alkali (NaOH). It was noted that with respect to P. mirabilis 4% alkali treated substrate yielded a higher percentage of H 2 (20.23 ± 0.03%) compared to the other two concentrations. The results indicate that alkali treated substrate produced comparatively higher amount of H 2 than that of acid treated substrates. Regarding efficiency, P. aeruginosa was found to be more competent than P. mirabilis.

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Aquatic biomass as sustainable feedstock for biorefineries

Kotamraju

Lens

2023

Biofuels Bioprod Bioref

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The development of biorefineries is a crucial step in the circular economy framework. In biorefineries, research is intensified towards utilizing feedstocks, which do not need arable land or compete with food sources. In this scenario, emerged, submerged and free-floating aquatic plants are garnering significant attention as potential feedstocks owing to their generation in huge quantities, especially in eutrophic water bodies, similar composition to lignocellulosic biomass with lower lignin content and requirement for only mild pre-treatments. Therefore, exploring the feasibility of using these aquatic plants for the production of various biocommodities in a biorefinery approach can be of prime importance. In light of this, the current review illustrates the use of some of the major aquatic plants for the production of different biocommodities. The main focus of the study is to shed light on the various biorefinery schemes that could be implemented using these aquatic plants. It also outlines the challenges and prospects of aquatic plant-based biorefineries. The findings suggest that various biorefinery schemes can be implemented using these aquatic plants and a combination of chemical and biological processes could aid in lowering the cost and achieving better yields. Furthermore, it is also observed that research on large-scale management and valorization of these aquatic plants also needs to be intensified.

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Bio-ethanol from water hyacinth biomass: An evaluation of enzymatic saccharification strategy

Cited by 136 publications

References 18 publications

Production of fermentable sugars by combined chemo-enzymatic hydrolysis of cellulosic material for bioethanol production

Production of fermentable sugars by combined chemo-enzymatic hydrolysis of cellulosic material for bioethanol production

Biohydrogen production by locally isolated facultative bacterial species using the biomass of Eichhornia crassipes: effect of acid and alkali treatment

Aquatic biomass as sustainable feedstock for biorefineries

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