Bio-fabrication of catalytic platinum nanoparticles and their in vitro efficacy against lungs cancer cells line (A549)

Ullah, Sadeeq; Ahmad, Aftab; Wang, Aoke; Raza, Muslim; Jan, Amin Ullah; Tahir, Kamran; Rаhmаn, Аziz Ur; Yuan, Qipeng

doi:10.1016/j.jphotobiol.2017.06.018

Cited by 41 publications

(23 citation statements)

References 36 publications

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“…This specificity could be attributed to the less compact cellular architecture of cancerous cells that allow penetration of AgNPs into cytoplasm of the cells. Similar findings have been reported by other researchers [20,43,44].…”

Section: Analysis Of Specificity and Cellular Uptake Of Agnps By Helasupporting

confidence: 93%

“…ICP-AES data suggested that diffusion might be the probable mechanism of AgNPs' entry into the cells; thus, it might lead the cancer cell to display apoptotic features and ultimately dies. A previous study indicated that AgNPs (under cell culture condition) displayed lesser endocytosis and clathrin pits formation [20].…”

Section: Analysis Of Specificity and Cellular Uptake Of Agnps By Helamentioning

confidence: 98%

“…The cells were exposed to 5, 10, 20, 40, 80 and 160 lg ml À1 of AgNPs, in culture media for 24 h and the viability of cells was determined by MTT assay. The cells were washed thrice with DMSO and added to each well having MTT solution (10%) and incubated for 5 h. The absorbance was measured @ 570 nm and the percent cell survival was determined as the percent MTT reduction by cells exposed to different concentrations of AgNPs relative to control group [20].…”

Section: Anticancer Activity Of Agnpsmentioning

confidence: 99%

“…These cells were treated with trypsin and collected by centrifugation (1600 rpm, for 5 min) and washed the cells with PBS thrice during centrifugation to remove extracellular AgNPs. Then resuspend the cells pellet in PBS (2 ml) and according to Privies protocol the samples were prepared for ICP-AES analysis [20].…”

Section: In Vitro Cellular Uptake Of Agnpsmentioning

confidence: 99%

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Biogenic synthesis of silver nanoparticles using extracts of Leptolyngbya JSC-1 that induce apoptosis in HeLa cell line and exterminate pathogenic bacteria

Zada

Ahmad

Khan

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

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Utilizing novel approaches for the green synthesis of metal nanoparticles are of great importance. Therefore, we reported biogenic synthesis of silver nanoparticles (AgNPs) using extracts of Leptolyngbya strain JSC-1, and their significant applications against pathogenic bacteria and cancerous HeLa cell line. The biofabricated AgNPs were characterized by UV-visible spectroscopy, FTIR, SEM, TEM, DLS and zeta-potential. The as prepared AgNPs were assessed for inhibition of bacterial growth and induction of apoptosis in HeLa cells by different doses of AgNPs was evaluated. UV-visible spectroscopy and FTIR of AgNPs demonstrated the surface plasmon resonance at 413 nm and interaction among extract and nanoparticles, respectively. Electron microscopy revealed the morphology and DLS demonstrated size distribution of the particles (10-100 nm). Zeta potential values were between -47 and 0 mV, indicating stability of the particles. Proliferation of HeLa cells was significantly inhibited and severe cytotoxicity with higher intracellular uptake were observed after applying high concentration of AgNPs. Efficient inhibition zones (17 ± 2 and 21 ± 2 mm) were produced at maximum concentration (100 µl from 1 mg ml stock of AgNPs) for Staphylococcus aureus and Escherichia coli, respectively. These findings reveal that the biofabricated AgNPs possess strong antibacterial activity and ability to induce apoptosis in cancer cell line (HeLa).

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Section: Analysis Of Specificity and Cellular Uptake Of Agnps By Helasupporting

confidence: 93%

Section: Analysis Of Specificity and Cellular Uptake Of Agnps By Helamentioning

confidence: 98%

Section: Anticancer Activity Of Agnpsmentioning

confidence: 99%

Section: In Vitro Cellular Uptake Of Agnpsmentioning

confidence: 99%

See 2 more Smart Citations

Biogenic synthesis of silver nanoparticles using extracts of Leptolyngbya JSC-1 that induce apoptosis in HeLa cell line and exterminate pathogenic bacteria

Zada

Ahmad

Khan

et al. 2018

Artificial Cells, Nanomedicine, and Biotechnology

Self Cite

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show abstract

“…According to the literature, the anticancer activity of platinum nanoparticles is associated with the inhibition of DNA replication and mitosis by the addition of platinum nanoparticles to the DNA strand (Sawosz et al 2010). Platinum nanoparticles penetrate into the nucleus and mitochondria, binding with DNA molecule and leading to cellular apoptosis (Ullah et al 2017).…”

Section: Resultsmentioning

confidence: 99%

Evaluation of biological synthesized platinum nanoparticles using Ononidis radix extract on the cell lung carcinoma A549

Dobrucka

Romaniuk-Drapała

Kaczmarek

2019

Biomed Microdevices

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Due to the search for new methods for synthesizing nanomaterials, this work proposes the biological synthesis of platinum nanoparticles using Ononidis radix extract. The synthesized platinum nanoparticles were characterized by UV-Vis, Scanning Electron Microscopy (SEM) with EDS profile, Fourier transform infrared spectroscopy (FTIR), Transmission Electron Microscopy (TEM) and Atomic Force Microscopy (AFM). The examination conducted by means of Transmission Electron Microscopy showed the presence of spherical and hexagonal platinum nanoparticles. Atomic Force Microscopy indicated the presence of locally agglomerated nanoparticles whose size was about 4 nm. The study also examined the influence of platinum nanoparticles on human non-small cell lung carcinoma cells A549. It was found that the mortality of cells cultured together with platinum nanoparticles increased, and the proliferative activity of A549 cells decreased gradually over time in proportion to the increasing concentration of the test substance. Graphical abstract

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Enhanced performance of methanol oxidation reaction via green synthesis of platinum electro‐catalyst from sugar cane bagasse

et al. 2021

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Summary Platinum‐based catalysts are regarded as premier anodic electrocatalysts in direct methanol fuel cells due to their great catalytic activities but hampered by its non‐benign conventional synthesis, and high production expenses. This study exploited a new sustainable pathway of a biosynthesis route using the biomass plant extract of sugarcane bagasse in a facile single‐step process guided by the principle of green chemistry performing enhanced functionalized bio‐platinum nanoparticles. The physiochemical characterization, along with the bio‐reductive mechanism were comprehensively discussed. The electrochemical activity was performed by cyclic voltammetry, chronoamperometry, and electrochemical impedance spectroscopy measurements. The novel nano‐spheroid biosynthesized Pt NPs manifested a high ECSA of approximately 95 m2/g with an enhanced current density of 400 mA/mgcatalyst−1, which were 3 to 4 times higher than those of Pt black commercial. The discovery of bioactive compounds from the dominant group of polyphenolics, flavonoids, carboxylic acids, and proteins suggested that they were accountable for the bio‐reductive mechanism.

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Bio-fabrication of catalytic platinum nanoparticles and their in vitro efficacy against lungs cancer cells line (A549)

Cited by 41 publications

References 36 publications

Biogenic synthesis of silver nanoparticles using extracts of Leptolyngbya JSC-1 that induce apoptosis in HeLa cell line and exterminate pathogenic bacteria

Biogenic synthesis of silver nanoparticles using extracts of Leptolyngbya JSC-1 that induce apoptosis in HeLa cell line and exterminate pathogenic bacteria

Evaluation of biological synthesized platinum nanoparticles using Ononidis radix extract on the cell lung carcinoma A549

Enhanced performance of methanol oxidation reaction via green synthesis of platinum electro‐catalyst from sugar cane bagasse

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