Bio-functionalized graphene–graphene oxide nanocomposite based electrochemical immunosensing

Abstract

We report a novel in-situ electrochemical synthesis approach for the formation of functionalized graphene–graphene oxide (fG–GO) nanocomposite on screen-printed electrodes (SPE). Electrochemically controlled nanocomposite film formation was studied by transmission electron microscopy (TEM) and Raman spectroscopy. Further insight into the nanocomposite has been accomplished by the Fourier transformed infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA) and X-ray diffraction (XRD) spectroscopy. Configured as a highly responsive screen-printed immunosensor, the fG–GO nanocomposite on SPE exhibits electrical and chemical synergies of the nano-hybrid functional construct by combining good electronic properties of functionalized graphene (fG) and the facile chemical functionality of graphene oxide (GO) for compatible bio-interface development using specific anti-diuron antibody. The enhanced electrical properties of nanocomposite biofilm demonstrated a significant increase in electrochemical signal response in a competitive inhibition immunoassay format for diuron detection, promising its potential applicability for ultra-sensitive detection of range of target analytes.

Synthesis and Characterization of Soluble Alkylalcohol-derivatized Graphene Oxide

“…These novel properties can be made graphene oxide greatly promising for the diverse applications including high functional batteries, sensors, solar energy system, and highly sophisticated microelectrical devices such as field-effect transistors and electromechanical resonators. [18][19][20][21][22][23][24][25][26][27] Here, therefore, we report a synthesis on derivatization of graphene oxide with alkylalcohol to give soluble graphenes. Because the soluble alkoxyderivatized graphene oxides may be presented a particular electronic transport properties.…”

High-performance electrochemical amperometric sensors for the sensitive determination of phenyl urea herbicides diuron and fenuron

“…Therefore, sensitive determination of DU is highly important. Several analytical techniques have been developed for the determination of DU, such as immunosensors [7,8], chromatography-mass spectrometry [9,10], highp e r f o r m a n c e l i q u i d c h r o m a t o g r a p h y a n d i o n i c Electronic supplementary material The online version of this article (doi:10.1007/s11581-015-1459-2) contains supplementary material, which is available to authorized users. chromatography [11], ultrahigh-pressure liquid chromatography triple quadrupole linear ion-trap mass spectrometry [12], and time-resolved fluoroimmunoassay [13]; however, most of these methods involve time-consuming and tedious procedures.…”

Graphene Oxide based Label Free Ultrasensitive Immunosensor for Lung Cancer Biomarker, hTERT

“…Besides this, as shown in Figure 5C, the linear regression equation: I pa (µA)=14.6986-1.2912 log C hTERT (ag mL -1 ) with R 2 =0.99318 is fitting in range of 100 fg mL -1 to 50 ng mL -1 hTERT. The fabricated Ab/GO/ITO immunoelectrode possess remarkable response characteristics showing 10 6 times improved detection limit with 30 s antigen exposure time [37]. Figure 5D shows DPV response curves of Ab/GO/ITO immunoelectrode exposed to non-specific cancer antigens (CD-59, MAGE-A11) 10 pg mL -1 each, in 5 mM PBS containing 0.05 mM K 3 Fe(CN) 6 .…”