Bio-Guided Isolation of the Cytotoxic Terpenoids from the Roots of Euphorbia kansui against Human Normal Cell Lines L-O2 and GES-1

Abstract

The dried roots of Euphorbia kansui (kansui) have been used for centuries in China as a herbal medicine for edema, ascites, and asthma. The 95% ethanol extract showed a significant inhibition of cell proliferation against human normal cell lines L-O2 and GES-1. Bioassay-guided separation of the 95% ethanol extract from the roots of E. kansui led to the isolation of 12 diverse terpenoids whose structures were identified by 1H, 13C NMR spectroscopy and ESI-MS as kansuinine A (1), kansuinine B (2), kansuinine C (3), kansuiphorin C (4), 3-O-(2′E,4′Z-decadienoyl)-20-O-acetylingenol (5), 3-O-(2′E,4′Edecadienoyl)-20-O-acetylingenol (6), 3-O-(2′E,4′Z-decadienoyl)-20-deoxyingenol (7), 3-O-benzoyl-20-deoxyingenol (8), 5-O-benzoyl-20-deoxyingenol (9), kansenone (10), epi-kansenone (11), euphol (12). All these 12 terpernoids were evaluated in vitro for cytotoxicity on L-O2 and GES-1 cell lines. Most ingenane-type diterpenoids and 8-ene-7-one triterpenoids (5–11) exhibited a relatively lower IC50 value; therefore, these compounds had stronger cytotoxicity against human normal cell lines L-O2 and GES-1 with dose-dependent relationships. These results will be significantly helpful to reveal the mechanism of toxicity of kansui and to effectively guide safer clinical application of this herb.

The occurrence of triterpenoids from Euphorbia gummifera inside the fairy circles of Garub in the southern Namibian pro-desert

“…NMR as well as mass spectrometry data of the isolated euphol correlated with previous literature reports (Lin et al, 2000;Zhang et al, 2012).…”

“…Lin et al, 2000;Zhang et al, 2012).2.4. Comparison of historical and recent satellite imagesGoogle earth satellite images of the collection site were obtained from the internet (accessed 2014-03-20) and visually compared for the remains of plants and the possible formation of new fairy circles from satellite images of2003, 2004, 2010, 2012 and 2013.…”

Optimizing Microwave‐Assisted Extraction Conditions to Obtain Phenolic‐Rich Extract from Chromolaena odorata Leaves

“…Different pharmacological activities of C. odorata leaf extract might be due to the combined action of these phenolic compounds. In a previous study, 3‐ O ‐benzoyl‐20‐deoxyingenol from Euphorbia kansui roots showed significant inhibition of cell proliferation against human normal cell lines .…”

Chemical Constituents from the Leaves of Euphorbia ammak Growing in Saudi Arabia

“…The result was in accordance with previous reports concerning the cytotoxic activity of euphol (1). [ 9 17 18 19 20 21 ] It is worthy to mention that euphol (1) was the only major compound found in the milky sap of leaves as detected by thin layer chromatography, while α-glutinol (2) and stigmasterol (3) were absent. This is the first report on the chemical investigation of E. ammak growing in Saudi Arabia.…”