Bio-guided profiling and HPLC-DAD finger printing of Atriplex lasiantha Boiss

Zohra, Tanzeel; Ovais, Muhammad; Khalil, Ali Talha; Qasim, Muhammad; Ayaz, Muhammad; Ahmad, Sajjad; Zahoor, Muhammad

doi:10.1186/s12906-018-2416-1

Cited by 41 publications

(28 citation statements)

References 51 publications

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“…Medicinal plants have attracted the attention of several stakeholders around the world [49]. They have chemical diversity and can play a signi cant role in new drug development [50]. In this study, the majority of respondents in Nepal reported that the use of medicinal plants has increased during COVID-19, they also believed that information about the medicinal plants has increased, and most of them recommend medicinal [20,51].…”

Section: Status and Sources Of Medicinal Plantmentioning

confidence: 70%

The Use of Medicinal Plant to Prevent COVID-19 in Nepal

Khadka

Dhamala

et al. 2020

Preprint

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Background: Medicinal plants are the fundamental unit of traditional medicine system in Nepal. Nepalese people are rich in traditional medicine especially in folk medicine (ethnomedicine) and this system is gaining much attention after 1995. The use of medicinal plants have increased during COVID-19 pandemic as a private behavior (not under the control of government). Lot of misinterpretations of the use of medicinal plants to treat or prevent COVID-19 have been spreading throughout Nepal which need to be managed proactively. In this context, a research was needed to document medicinal plants used, their priority of use in society, cultivation status and source of information people follow to use them. This study aimed to document the present status of medicinal plant use and make important suggestion to the concern authorities.Methods: This study used a web-based survey to collect primary data related to medicinal plants used during COVID-19. A total of 774 respondents took part in the survey. The study calculated the relative frequencies of citation (RFC) for the recorded medicinal plants. The relationship between plants recorded and different covariates (age, gender education, occupation, living place, and treatment methods were assessed using Kruskal Wallis test and Wilcox test. The relationship between the information sources people follow and respondent characteristics, were assessed using chi-square test.Results:The study found that the use of medicinal plants has increased during COVID-19 and most of the respondents recommended medicinal plants to prevent COVID-19. This study recorded a total of 63 plants belonging to 42 families. The leaves of the plants were the most frequently used. The Zingiber officinale was the most cited species with the frequency of citation 0.398. Most of the people (45.61%), were getting medicinal plants from their home garden. The medicinal plants recorded were significantly associated with the education level, location of home, primary treatment mode, gender, and age class. The information source of plants was significantly associated with the education, gender, method of treatment, occupation, living with family, and location of home during lockdown caused by COVID-19.Conclusions:People were using more medicinal plants during COVID-19 claiming that they can prevent or cure COVID-19. This should be taken seriously by concerned authorities. The authorities should test the validity of these medicinal plants and control the flow of false information spread through research and awareness programs.

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Section: Status and Sources Of Medicinal Plantmentioning

confidence: 70%

The Use of Medicinal Plant to Prevent COVID-19 in Nepal

Khadka

Dhamala

et al. 2020

Preprint

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“…In addition, R. virgate ‐inspired IONPs, AA inhibition potential was performed in the range of 1000–31.25 μg/ml. The AA is responsible for converting carbohydrates into glucose, therefore blocking the function of AA can prevent the level of glucose, which is the main area of diabetes research . Bioinspired IONPs were investigated for their AA inhibition potential.…”

Section: Resultsmentioning

confidence: 99%

Biofabrication of iron oxide nanoparticles by leaf extract of Rhamnus virgata: Characterization and evaluation of cytotoxic, antimicrobial and antioxidant potentials

Abbasi

Iqbal

Mahmood

et al. 2019

Applied Organom Chemis

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In the present study, plant‐mediated synthesis of iron oxide nanoparticles (IONPs) using leaves extract of Rhamnus virgata (Roxb.) as a potential stabilizing, reducing and chelating agent is reported. The biogenic IONPs are extensively characterized for their physical and biological properties. The morphology, structure and physicochemical properties of biogenic IONPs were characterized using ultraviolet spectroscopy, X‐ray diffraction, Fourier transform‐infrared analysis, scanning electron microscopy, energy‐dispersive spectroscopy, transmission electron microscopy, Raman spectroscopy and dynamic light scattering. The Scherrer equation deduced a mean crystallite size of ~20 nm for IONPs. Detailed in vitro biological activities revealed significant therapeutic potentials for IONPs. Potential antibacterial and antifungal activities are reported for IONPs. Bioinspired IONPs have shown potential results against HepG2 cells (IC50: 13.47 μg/ml). Dose‐dependent cytotoxicity assays were revealed against Leishmania tropica (KMH23) promastigotes (IC50: 8.08 μg/ml) and amastigotes (IC50: 20.82 μg/ml) using different concentrations of IONPs (1–200 μg/ml). The cytotoxic activity was also studied using brine shrimps, and their IC50 value was calculated as 32.41 μg/ml. Significant antioxidant [TAC (51.4%), DPPH (79.4%) and total reducing power (62%)], protein kinase and alpha amylase inhibition assays were revealed. The biocompatibility assays using red blood cells (> 200 μg/ml) and macrophages (> 200 μg/ml) confirmed the biosafe nature of IONPs. In conclusion, bioinspired IONPs have shown potential biological applications and should be subjected to further research work to develop their nano‐pharmacological relevance in biomedical applications.

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“…The resultant mixture was incubated at 90 °C for 95 min and absorbance were recorded at 645 nm via UV spectrophotometer. Ascorbic acid was used as positive control and antioxidant capacity was expressed as µg AAE/mg extract [38].…”

Section: Methodsmentioning

confidence: 99%

Phytochemical Analysis, Ephedra Procera C. A. Mey. Mediated Green Synthesis of Silver Nanoparticles, Their Cytotoxic and Antimicrobial Potentials

et al. 2019

Self Cite

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: Background and Objectives: The current study focuses on an eco-friendly and cost-effective method of Ephedra procera C. A. Mey. mediated green synthesis of silver nanoparticles as potential cytotoxic, antimicrobial and anti-oxidant agents. Materials and Methods: Plant aqueous extracts were screened for Total Phenolic (TPC), Total Flavonoid contents (TFC), Total Antioxidant Capacity (TAC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging potentials. Total reducing power estimated by potassium ferricyanide colorimetric assay. The biosynthesized E. procera nanoparticles (EpNPs) were characterized by UV-spectroscopy, Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction and Scanning electron microscopy. EpNPs were evaluated for their antimicrobial, bio-compatibility and cytotoxic potentials. Results: Initial phytocheimcal analysis of plant aqueous extract revealed TFC of 20.7 ± 0.21 µg/mg extract and TPC of 117.01 ± 0.78 µg/mg extract. TAC, DPPH free radical scavenging and reducing power were 73.8 ± 0.32 µg/mg extract, 71.8 ± 0.73% and 105.4 ± 0.65 µg/mg extract respectively. The synthesized EpNPs were observed to possess high cytotoxicity against HepG2 cancer cell lines with IC50 (61.3 µg/mL) as compared aqueous extract with IC50 of (247 µg/mL). EpNPs were found to be biocompatible and have less effect on human erythrocytes. EpNPs exhibited significant antioxidant potentials and exhibited considerable activity against Escherichia coli and Bacillus subtilis with Minimum Inhibitory Concentration (MICs) of 11.12 μg/mL and 11.33 μg/mL respectively. Fungal species Aspergillus niger and Aspergillus flavus were found susceptible to EpNPs. Conclusions: Results of the current study revealed that EpNPs exhibited considerable antibacterial, antifungal and cytotoxic potentials. Aqueous extract possesses significant anti-radical properties and thus can be useful in free radicals induced degenerative disorders.

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Bio-guided profiling and HPLC-DAD finger printing of Atriplex lasiantha Boiss

Cited by 41 publications

References 51 publications

The Use of Medicinal Plant to Prevent COVID-19 in Nepal

The Use of Medicinal Plant to Prevent COVID-19 in Nepal

Biofabrication of iron oxide nanoparticles by leaf extract of Rhamnus virgata: Characterization and evaluation of cytotoxic, antimicrobial and antioxidant potentials

Phytochemical Analysis, Ephedra Procera C. A. Mey. Mediated Green Synthesis of Silver Nanoparticles, Their Cytotoxic and Antimicrobial Potentials

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