Bio-inorganic hybrid structures for direct electron transfer to photosystem I in photobioelectrodes

Closing the green gap of photosystem I with synthetic fluorophores for enhanced photocurrent generation in photobiocathodes

“…15,37,38 For the latter, it has been shown recently by ourselves that conditions can be found to increase the performance significantly. 58…”

“…In contrast to previous studies with photobiocathodes, the buffer D was used for the PSI solution to nd conditions suitable for all constructs and the unmodied PSI. 37,56,58 Finally, the PSI solutions were set to 20 mM and 4 mM, respectively, and kept at 4 °C.…”

“…Compared to earlier studies, the protein incubation was slightly modied. 37,58 It was always performed for 3 min in the dark, and excessive biomolecules were removed by rinsing twice with 1 mL buffer as described below. For the test series exploiting MET, 3 mL of 20 mM PSI and 5 mL of 1 mM cytochrome c (cyt c, in buffer E (5 mM PPB pH 7)) solutions were utilized and buffer E was applied for rinsing and the execution of the electrochemical measurements.…”

“…This procedure allows tuning the thickness in the range of 10 to 30 mm providing thus an attractive 3D space for PSI immobilization. 58 The open 3D structures have been veried by SEM investigations as given in Fig. S4.…”

“…15,37,38 For the latter, it has been shown recently by ourselves that conditions can be found to increase the performance signicantly. 58 For the immobilization of the native protein and the constructs, direct assembly on 3D ITO has been exploited which is fast and efficient. The same is valid for cyt c deposition.…”