Bio‐Inspired Integration of Natural Materials

Martins, Albino; Silva, Marta Alves da; Costa-Pinto, Ana Rita; Reis, Rui L.; Neves, Nuno M.

doi:10.1002/9781118843499.ch8

Cited by 7 publications

(3 citation statements)

References 175 publications

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“…To better discuss this important issue, the denaturation temperature of collagen needs to be further investigated. It is important to highlight that the thermal stability of the triple helix collagen is of fundamental importance, since collagen is an essential structural compound that has the ability to support the skin and to strengthen the elastic sheets and tendons [12,25,47].…”

Section: Amino Acid Analysismentioning

confidence: 99%

“…Also, it can provide flexibility, elasticity, mechanical stability, and hydration (natural humectant and moisturizer) [23]. It can be easily processed and combined with other biomaterials [7,[24][25][26][27], providing a more complex biomimetic character to the final architecture. On that research trend, chitosan and fucoidan were pointed as good candidates for a combinatorial approach [3,26].…”

Section: Introductionmentioning

confidence: 99%

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Marine collagen-chitosan-fucoidan cryogels as cell-laden biocomposites envisaging tissue engineering

et al. 2020

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The combination of marine origin biopolymers for tissue engineering (TE) applications is of high interest, due to their similarities with the proteins and polysaccharides present in the extracellular matrix of different human tissues. This manuscript reports on innovative collagen-chitosan-fucoidan cryogels formed by the simultaneous blending of these three marine polymers in a chemical-free crosslinking approach. The physicochemical characterization of marine biopolymers comprised FTIR, amino acid analysis, circular dichroism and SDS-PAGE, and suggested that the jellyfish collagen used in the cryogels was not denatured (preserved the triple helical structure) and had similarities with type II collagen. The chitosan presented a high deacetylation degree (90.1%) that can strongly influence the polymer physicochemical properties and biomaterial formation. By its turn, rheology, and SEM studies confirmed that these novel cryogels present interesting properties for TE purposes, such as effective blending of biopolymers without visible material segregation, mechanical stability (strong viscoelastic character), as well as adequate porosity to support cell proliferation and exchange of nutrients and waste products. Additionally, in vitro cellular assessments of all cryogel formulations revealed a non-cytotoxic behavior. The MTS test, live/dead assay and cell morphology assessment (phalloidin DAPI) showed that cryogels can provide a proper microenvironment for cell culturing, supporting cell viability and promoting cell proliferation. Overall, the obtained results suggest that the novel collagen-chitosan-fucoidan cryogels herein presented are promising scaffolds envisaging tissue engineering purposes, as both acellular biomaterials or cell-laden cryogels.

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Section: Amino Acid Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Marine collagen-chitosan-fucoidan cryogels as cell-laden biocomposites envisaging tissue engineering

et al. 2020

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“…Additionally, chitosan has been used for tissue engineering in humans since it structurally shares a monomer with hyaluronic acid found in ECM, especially in cartilage tissues [ 22 ]. Being mainly obtained from chitin present in crustacean shells (i.e., shrimps and crabs) and squid pens, this polymer contains remarkable properties such as biodegradability, biocompatibility, low toxicity, anti-inflammatory and antibacterial, making it a potential candidate for cartilage tissue repair [ 23 , 24 ]. In the same way, fucoidan is commonly considered to resemble sulfated glycosaminoglycan (such as chondroitin sulfates) found in ECM [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

Advanced Polymeric Membranes as Biomaterials Based on Marine Sources Envisaging the Regeneration of Human Tissues

Carvalho

Lobo

Rodrigues

et al. 2023

Gels

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The self-repair capacity of human tissue is limited, motivating the arising of tissue engineering (TE) in building temporary scaffolds that envisage the regeneration of human tissues, including articular cartilage. However, despite the large number of preclinical data available, current therapies are not yet capable of fully restoring the entire healthy structure and function on this tissue when significantly damaged. For this reason, new biomaterial approaches are needed, and the present work proposes the development and characterization of innovative polymeric membranes formed by blending marine origin polymers, in a chemical free cross-linking approach, as biomaterials for tissue regeneration. The results confirmed the production of polyelectrolyte complexes molded as membranes, with structural stability resulting from natural intermolecular interactions between the marine biopolymers collagen, chitosan and fucoidan. Furthermore, the polymeric membranes presented adequate swelling ability without compromising cohesiveness (between 300 and 600%), appropriate surface properties, revealing mechanical properties similar to native articular cartilage. From the different formulations studied, the ones performing better were the ones produced with 3 % shark collagen, 3% chitosan and 10% fucoidan, as well as with 5% jellyfish collagen, 3% shark collagen, 3% chitosan and 10% fucoidan. Overall, the novel marine polymeric membranes demonstrated to have promising chemical, and physical properties for tissue engineering approaches, namely as thin biomaterial that can be applied over the damaged articular cartilage aiming its regeneration.

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Recapitulation of Thymic Function by Tissue Engineering Strategies

Silva

Reis

Martins

et al. 2021

Adv Healthcare Materials

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The thymus is responsible for the development and selection of T lymphocytes, which in turn also participate in the maturation of thymic epithelial cells. These events occur through the close interactions between hematopoietic stem cells and developing thymocytes with the thymic stromal cells within an intricate 3D network. The complex thymic microenvironment and function, and the current therapies to induce thymic regeneration or to overcome the lack of a functional thymus are herein reviewed. The recapitulation of the thymic function using tissue engineering strategies has been explored as a way to control the body's tolerance to external grafts and to generate ex vivo T cells for transplantation. In this review, the main advances in the thymus tissue engineering field are disclosed, including both scaffold-and cell-based strategies. In light of the current gaps and limitations of the developed systems, the design of novel biomaterials for this purpose with unique features is also discussed.

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Bio‐Inspired Integration of Natural Materials

Cited by 7 publications

References 175 publications

Marine collagen-chitosan-fucoidan cryogels as cell-laden biocomposites envisaging tissue engineering

Marine collagen-chitosan-fucoidan cryogels as cell-laden biocomposites envisaging tissue engineering

Advanced Polymeric Membranes as Biomaterials Based on Marine Sources Envisaging the Regeneration of Human Tissues

Recapitulation of Thymic Function by Tissue Engineering Strategies

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