Bio-inspired synthesis and characterizations of groundnut shells-mediated Cu/CuO/Cu2O nanoparticles for anticancer, antioxidant, and DNA damage activities

Shinde, Shraddha; Parjane, Shradha; Turakane, Harshada; Basnet, Parita; Oza, Rajeshwari; Abhale, Yogita K.; Pansambal, Shreyas; Khoo, Kuan Shiong; Rahdar, Abbas; Ghotekar, Suresh

doi:10.1007/s10971-023-06109-7

Cited by 16 publications

(1 citation statement)

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“…No other heteroploidy, polyploidy, or chromosomal translocation was observed. Therefore, the representative karyotype of HCC-ZJ was described as 46, XY, del [ 8 ](p11.2) Figure 1(c) .…”

Section: Resultsmentioning

confidence: 99%

Establishment and characterization of a highly metastatic hepatocellular carcinoma cell line

Huang,

Sun,

Wang

et al. 2024

Bioengineered

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The prevalence of alcohol-related hepatocellular carcinoma (HCC) has been increasing during the last decade. Cancer research requires cell lines suitable for both in vitro and in vivo assays. However, there is a lack of cell lines with a high in vivo metastatic capacity for this HCC subtype. Herein, a new HCC cell line was established, named HCC-ZJ, using cells from a patient diagnosed with alcohol-related HCC. The karyotype of HCC-ZJ was 46, XY, del (p11.2). Whole-exome sequencing identified several genetic variations in HCC-Z that occur frequently in alcohol-associated HCC, such as mutations in TERT , CTNNB1 , ARID1A , CDKN2A , SMARCA2 , and HGF . Cell counting kit-8 assays, colony formation assays, and Transwell assays were performed to evaluate the proliferation, migration, and sensitivity to sorafenib and lenvatinib of HCC-Z in vitro . HCC-ZJ showed a robust proliferation rate, a weak foci-forming ability, a strong migration capacity, and a moderate invasion tendency in vitro . Finally, the tumorigenicity and metastatic capacity of HCC-Z were evaluated using a subcutaneous xenograft model, an orthotopic xenograft model, and a tail-veil injection model. HCCZJ exhibited strong tumorigenicity in the subcutaneous xenograft and orthotopic tumor models. Moreover, HCC-ZJ spontaneously formed pulmonary metastases in the orthotopic tumor model. In summary, a new HCC cell line derived from a patient with alcohol-related HCC was established, which showed a high metastatic capacity and could be applied for in vitro and in vivo experiments during pre-clinical research. Highlights • An alcohol-related HCC cell line, HCC-ZJ, was established • HCC-ZJ was applicable for in vitro functional experiment and gene editing • HCC-ZJ was applicable for in vivo tumor growth and spontaneous metastasis models

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“…No other heteroploidy, polyploidy, or chromosomal translocation was observed. Therefore, the representative karyotype of HCC-ZJ was described as 46, XY, del [ 8 ](p11.2) Figure 1(c) .…”

Section: Resultsmentioning

confidence: 99%