The study and application of microscale lenses and lens arrays have been actively researched in recent years; new approaches in the fabrication of microlenses and microlens arrays have emerged. Also, novel applications of these microlenses and microlens arrays have been demonstrated. In an effort to disseminate the current advances in this specialized field of microlenses and microlens arrays, and to encourage discussion on the future research directions while stimulating research interests in this area, a Special Issue of Micromachines has been dedicated to "Microlenses".This Special Issue presents a total of ten papers covering most of the active areas of research in microlenses and microlens arrays. Specifically, eight papers are on the fabrication and characterization of microlenses and microlens arrays, one focuses on integration of microlenses into complex micro-optical modules, and the last one deals with the super-hydrophobic surface that is often important to realize droplet-based microlenses.The fabrication and realization of microlenses and microlens arrays reported in these papers, cover a wide range of mechanisms, technologies, materials, and optical designs. Xu et al.[1] provide a thorough review of how to improve the response time of liquid crystal (LC) based tunable-focus microlenses. The basic operating principles and recent progress are introduced and reviewed for two types of fast-response microlenses based on LC/polymer composites: polymer dispersed/stabilized nematic LC and polymer-stabilized blue phase LC. Chen et al. [2] propose a polarization independent LC microlens arrays based on controlling the spatial distribution of the Kerr constants of blue phase LC; the concept is supported by simulation. Two papers are on adaptive liquid lenses actuated via electrowetting. Liu et al.