Bio-inspired ZnO nanoparticles from Ocimum tenuiflorum and their in vitro antioxidant activity

Sushma, N. John; Mahitha, B.; Mallikarjuna, K.; Raju, B. Deva Prasad

doi:10.1007/s00339-016-0069-9

Cited by 36 publications

(21 citation statements)

References 46 publications

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“…Previous studies on ZnO nanopellets exhibited significant activity (IC 50 value 11.40 lg/mL) at higher concentration. ZnO NPs synthesized by O. tenuiflorum act as potential antioxidants [49]. Nanoparticles of gold [50], silver [51], selenium [52] and copper oxide [53] are known to exhibit free radical scavenging activity.…”

Section: Antioxidant Activity Of Zno Nanoparticlesmentioning

confidence: 99%

Phytoextract-mediated synthesis of zinc oxide nanoparticles using aqueous leaves extract of Ipomoea pes-caprae (L).R.br revealing its biological properties and photocatalytic activity

Venkateasan

Prabakaran

Sujatha

2017

Nanotechnol. Environ. Eng.

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ZnO NPs were efficiently synthesized from the leaves extract of Ipomoea pes-caprae. The work focuses on phytochemical studies, phytomediated synthesis and characterization of ZnO NPs and its biological applications. Phytochemicals present in the extract may be responsible for reducing the metal salts into their respective nanosize particles. The UV-Vis spectrum infers the formation of NPs, with absorbed peak at 322 nm. The XRD pattern depicted significant peaks at 2h positions which confirmed the hexagonal wurtzite structure. FTIR spectrum (ZnO NPs) was recorded to identify the functional group of biomolecules involved in synthesis. ZnO NPs was measured in the range of 2-20 nm by FE-SEM and particle analyses. ZnO NPs in various concentrations revealed potential antioxidant (IC 50 -21.09) and antibacterial activities. Cytotoxicity activity by Vero cell line (MTT assay) was found to be significant. ZnO NPs exhibited concentration-dependent cytotoxic reactivity to Vero cells after 24-h contact. ZnO NPs exhibited efficient dye degradation of methylene blue in the presence of sunlight. Due to the versatile properties and applications of ZnO NPs, these were widely studied by user-friendly approaches being green, simple and economy.& Venugopal Sujatha

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Section: Antioxidant Activity Of Zno Nanoparticlesmentioning

confidence: 99%

Phytoextract-mediated synthesis of zinc oxide nanoparticles using aqueous leaves extract of Ipomoea pes-caprae (L).R.br revealing its biological properties and photocatalytic activity

Venkateasan

Prabakaran

Sujatha

2017

Nanotechnol. Environ. Eng.

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“…Due to their low toxicity and size dependent properties, ZnO NPs have been widely used for various applications in textiles, cosmetics, diagnostics and even in micro-electronics. Because ZnO is generally recognized as safe (GRAS) and exhibits antimicrobial properties, ZnO NPs hold greater potential to treat infectious diseases in humans and animals 11 .…”

mentioning

confidence: 99%

“…For example, when Olea europea leaf extract was used to synthesize ZnO nano sheets, it ranged from 18-30 nm in size 14 . However, when Aloe barbadensis 15 and Ocimum tenuiflorum 11 were used as reducing agent for the green synthesis of ZnO nanoparticles, the average nanoparticle sizes were 25-40 nm and 13.86 nm respectively. Recently various reports have also demonstrated the antimicrobial activity of ZnO NPs.…”

mentioning

confidence: 99%

Green route to synthesize Zinc Oxide Nanoparticles using leaf extracts of Cassia fistula and Melia azadarach and their antibacterial potential

Naseer

Aslam

Khalid

et al. 2020

Sci Rep

461

137

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Development of plant based nanoparticles has many advantages over conventional physico-chemical methods and has various applications in medicine and biology. in present study, zinc oxide (Zno) nanoparticles (nps) were synthesized using leaf extracts of two medicinal plants Cassia fistula and Melia azadarach. 0.01 M zinc acetate dihydrate was used as a precursor in leaf extracts of respective plants for NPs synthesis. The structural and optical properties of NPs were investigated by X-ray diffraction (XRD), fourier transform infrared (ftiR) spectroscopy, scanning electron microscope (SeM), ultraviolet-visible spectrophotometer (UV-Vis) and dynamic light scattering (DLS). the antibacterial potential of Zno NPs was examined by paper disc diffusion method against two clinical strains of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) based on the zone of inhibition and minimal inhibitory indices (Mic). change in color of the reaction mixture from brown to white indicated the formation of Zno NPs. UV peaks at 320 nm and 324 nm, and XRD pattern matching that of JCPDS card for ZnO confirmed the presence of pure ZnO NPs. FTIR further confirmed the presence of bioactive functional groups involved in the reduction of bulk zinc acetate to Zno nps. SeM analysis displayed the shape of nps to be spherical whereas DLS showed their size range from 3 to 68 nm. The C. fistula and M. azadarach mediated Zno nps showed strong antimicrobial activity against clinical pathogens compared to standard drugs, suggesting that plant based synthesis of nps can be an excellent strategy to develop versatile and eco-friendly biomedical products. Plant mediated synthesis of nanoparticles (NPs) is a revolutionary technique that has wide range of applications in agriculture, food industry and medicine. NPs synthesized via conventional methods have limited uses in clinical domain due to their toxicity. Due to the physio-chemical properties of plant based NPs, this method also offer an added advantage of increased life span of NPs that overcome the limitations of conventional chemical and physical methods of NPs synthesis 1-3. Plants possess rich genetic variability with respect to number of biomolecules and metabolites like proteins, vitamins, coenzymes based intermediates, phenols, flavonoids and carbohydrates. These plant metabolites contain hydroxyl, carbonyl, and amine functional groups that react with metal ions and reduce their size into nano range. More specifically, flavonoids contain several functional groups and it is believed that-OH group of flavonoids is mainly considered responsible for the reduction of metal ions into NPs 4. These molecules not only help in bioreduction of the ions to the nano scale size, but they also play a pivotal role in the capping of the nanoparticles which is important for stability and biocompatibility 5. Reducing agents such as phenolic compounds, sterols and alkaloids can reduce metal ions into NPs in a single reaction 6 .

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“…These results are in agreement with previous reports. 58,62 Absence of any extra peak in EDX spectra of both types of synthesized ZnO NPs, conrms the formation of pure ZnO NPs. 63…”

Section: Sem and Edx Analysis Of Ce And Re-mediated Zno Npsmentioning

confidence: 99%

“…The possible mechanism involved in the biosynthesis of ZnO NPs by using plant extracts is not fully explored and yet under debate. 62 However, many studies have reported the involvement of plant metabolites containing polar groups in reduction and stabilization of metal oxide nanoparticles. 64,65 In present study, FTIR analysis of CE and RE-mediated ZnO NPs revealed that the polyphenols, aromatic and carbonyl compounds are mainly responsible for reduction and surface capping of ZnO NPs.…”

Section: Probable Mechanism Involved In Biosynthesis Of Zno Npsmentioning

confidence: 99%

Differential effects of in vitro cultures of Linum usitatissimum L. (Flax) on biosynthesis, stability, antibacterial and antileishmanial activities of zinc oxide nanoparticles: a mechanistic approach

2017

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Bio-inspired ZnO nanoparticles from Ocimum tenuiflorum and their in vitro antioxidant activity

Cited by 36 publications

References 46 publications

Phytoextract-mediated synthesis of zinc oxide nanoparticles using aqueous leaves extract of Ipomoea pes-caprae (L).R.br revealing its biological properties and photocatalytic activity

Phytoextract-mediated synthesis of zinc oxide nanoparticles using aqueous leaves extract of Ipomoea pes-caprae (L).R.br revealing its biological properties and photocatalytic activity

Green route to synthesize Zinc Oxide Nanoparticles using leaf extracts of Cassia fistula and Melia azadarach and their antibacterial potential

Differential effects of in vitro cultures of Linum usitatissimum L. (Flax) on biosynthesis, stability, antibacterial and antileishmanial activities of zinc oxide nanoparticles: a mechanistic approach

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