Bio-metals imaging and speciation in cells using proton and synchrotron radiation X-ray microspectroscopy

Ortega, Richard; Devès, Guillaume; Carmona, Asunción

doi:10.1098/rsif.2009.0166.focus

Cited by 141 publications

(133 citation statements)

References 59 publications

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“…The use of direct analytical methods for chemical element imaging offers the possibility to identify the subcellular compartments where trace elements are located and to quantify their content, without almost any sample preparation and avoiding any possible contamination, redistribution, or loss of trace elements (17).…”

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confidence: 99%

Manganese Accumulates within Golgi Apparatus in Dopaminergic Cells as Revealed by Synchrotron X-ray Fluorescence Nanoimaging

Carmona¹,

Devès²,

Roudeau³

et al. 2009

ACS Chem. Neurosci.

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Chronic exposure to manganese results in neurological symptoms referred to as manganism and is identified as a risk factor for Parkinson's disease. In vitro, manganese induces cell death in the dopaminergic cells, but the mechanisms of manganese cytotoxicity are still unexplained. In particular, the subcellular distribution of manganese and its interaction with other trace elements needed to be assessed. Applying synchrotron X-ray fluorescence nanoimaging, we found that manganese was located within the Golgi apparatus of PC12 dopaminergic cells at physiologic concentrations. At increasing concentrations, manganese accumulates within the Golgi apparatus until cytotoxic concentrations are reached resulting in a higher cytoplasmic content probably after the Golgi apparatus storage capacity is exceeded. Cell exposure to manganese and brefeldin A, a molecule known to specifically cause the collapse of the Golgi apparatus, results in the striking intracellular redistribution of manganese, which accumulates in the cytoplasm and the nucleus. These results indicate that the Golgi apparatus plays an important role in the cellular detoxification of manganese. In addition manganese exposure induces a decrease in total iron content, which could contribute to the overall neurotoxicity.

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mentioning

confidence: 99%

Manganese Accumulates within Golgi Apparatus in Dopaminergic Cells as Revealed by Synchrotron X-ray Fluorescence Nanoimaging

Carmona¹,

Devès²,

Roudeau³

et al. 2009

ACS Chem. Neurosci.

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“…This technique can detect elements at low concentrations, and has helped clarify the intracellular distribution of trace elements in many cell types, including phagocytic cells [3]. One major advantage that XFM has over related techniques used to study intracellular chemical reactions is that mammalian cells can be studied without chemical fixation and with minimal preparation.…”

Section: Introductionmentioning

confidence: 99%

Examination of trafficking of phagocytosed colloid particles in neutrophils using synchrotron-based X-ray fluorescence microscopy (XFM)

et al. 2011

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Synchrotron-based X-ray fluorescence microscopy (XFM) can localise chemical elements at a subcellular level. 99mTechnetium stannous (TcSn) colloid is taken up by phagocytes via a Complement Receptor 3 mediated phagocytic process. In the current study, XFM was used to examine the intracellular trafficking of TcSn colloid in neutrophils. XFM was performed on TcSn colloid, and neutrophils labelled with TcSn colloid, in whole blood. We developed a set of pixel by pixel analysis and mapping techniques incorporating cluster analysis that allowed us to differentiate neutrophils and artefactual contaminants, and we examined the changes in element distribution that accompany neutrophil phagocytosis of TcSn colloid. Sn became associated with half the neutrophils. Within cells, Sn colocalised with iron (Fe) and sulphur (S), and was negatively associated with calcium (Ca). Despite the high sensitivity of XFM, Tc was not detected. XFM can help clarify the intracellular processes that accompany neutrophil phagocytosis. The subcellular colocalisation of Sn with Fe is consistent with fusion of the colloid-containing phagosome with neutrophil Electronic supplementary material The online version of this article

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“…This technique can provide semi-quantitative or quantitative information since the fluorescence intensity is related to the concentration of the element within the sample. Over the past decade, the investigation of biological samples with XRF has been boosted by the development of high-flux and highly focused X-ray beam at different synchrotron facilities [32][33][34].…”

Section: Abnormalities In the Adventitia Layermentioning

confidence: 99%

The Pathology of the Internal Jugular Vein Wall in Multiple Sclerosis

Pedriali¹,

Zamboni²

2015

J Mult Scler

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In the last years it has been described a condition named Chronic Cerebrospinal Venous Insufficiency (CCSVI), frequently but not exclusively associated to Multiple Sclerosis (MS), which generated a strong scientific controversy about the epidemiological prevalence and the possible role in the complex, multi-factorial MS ethio-pathogenesis.However, CCSVI description also stimulated a considerable research activity on the extracranial veins. Among the fields of interest, the pathology of the Internal Jugular Veins (IJVs) was deeply investigated by some groups, so improving our knowledge in an underestimated field of MS research. Currently, the available papers clearly show the presence of abnormalities in the IJV wall of MS patient's respect to control tissue. In the tunica intima a significant derangement and loss of the endothelial cells have been described. Interestingly scanning electronic microscopy showed absence of endothelium in the defective jugular valves. In the adventitia it has been described an inverted ratio between type I and type III collagen, with prevalence of the latter. Finally, in the adventitia layer it has been found the presence of calcifications arranged around the vena venarum. Assessment of immune cells in the three IJV layers did not demonstrate increased infiltration. Current studies do not clarify the origin of the pathology of the IJV in patients with MS. Congenital, infectious, or even post thrombotic ethiology have been advocated. Finally, the review summarizes studies which link the CCSVI pathophysiology to the complex MS pathogenesis, and particularly to the impact of restricted brain outflow on the cerebral spinal fluid dynamics and cerebral perfusion.

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Bio-metals imaging and speciation in cells using proton and synchrotron radiation X-ray microspectroscopy

Cited by 141 publications

References 59 publications

Manganese Accumulates within Golgi Apparatus in Dopaminergic Cells as Revealed by Synchrotron X-ray Fluorescence Nanoimaging

Manganese Accumulates within Golgi Apparatus in Dopaminergic Cells as Revealed by Synchrotron X-ray Fluorescence Nanoimaging

Examination of trafficking of phagocytosed colloid particles in neutrophils using synchrotron-based X-ray fluorescence microscopy (XFM)

The Pathology of the Internal Jugular Vein Wall in Multiple Sclerosis

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