Bio-microfluidic platform for gold nanoprobe based DNA detection—application to Mycobacterium tuberculosis

Bernacka-Wojcik, Iwona; Lopes, Pedro Faria; Vaz, Ana; Veigas, Bruno; Wójcik, P.; Simões, Pedro; Barata, David; Fortunato, Elvira; Baptista, Pedro V.; Águas, Hugo; Martins, Rodrigo

doi:10.1016/j.bios.2013.03.079

Cited by 42 publications

(32 citation statements)

References 32 publications

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“…Considering application at POC, Baptista and colleagues integrated this non-cross-linking method in an optoelectronic platform (an amorphous/nanocrystalline biosensor and a light emission source) that assesses the colorimetric changes. This low-cost simple platform may be of use once integrated in a microfluidic test device for improved TB diagnostics with a tenfold reduction of reagents [25][26][27]. Recently, in an effort to increase sensitivity and ease of use, this detection strategy was integrated onto a paper-based platform [28].…”

Section: Aunps For Colorimetric Sensingmentioning

confidence: 97%

Gold Nanoparticles for DNA/RNA-Based Diagnostics

Franco¹,

Pedrosa²,

Carlos³

et al. 2015

Handbook of Nanoparticles

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The remarkable physicochemical properties of gold nanoparticles (AuNPs) have prompted development in exploring biomolecular interactions with AuNPs-containing systems, pursuing biomedical applications in diagnostics. Among these applications, AuNPs have been remarkably useful for the development of DNA/RNA detection and characterization systems for diagnostics, including systems suitable for point of need. Here, emphasis will be on available molecular detection schemes of relevant pathogens and their molecular characterization, genomic sequences associated with medical conditions (including cancer), mutation and polymorphism identification, and the quantification of gene expression.

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Section: Aunps For Colorimetric Sensingmentioning

confidence: 97%

Gold Nanoparticles for DNA/RNA-Based Diagnostics

Franco¹,

Pedrosa²,

Carlos³

et al. 2015

Handbook of Nanoparticles

Self Cite

View full text Add to dashboard Cite

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“…The reaction on the poly(dimethylsiloxane) microchip required only a small sample volume (3 ml) and discrimination of the target DNA was possible within 5 min with high sensitivity values. In a separate study, a poly(dimethylsiloxane) chip has also been successfully applied for the DNA detection of M. tuberculosis using only 3 ml of DNA solution [100]. Another inspiring example refers to the Gold-on-Paper platform [101], a paper-based analytical tool suitable for application in diagnostics at point of need, which combines the colorimetric changes of the NCL Au-nanoprobe assay with inexpensive paper-based platforms and the digital image analysis to screen for MTBC members.…”

Section: Point-of-need Devicesmentioning

confidence: 99%

Gold nanoprobe-based non-crosslinking hybridization for molecular diagnostics

Larguinho

Canto

Cordeiro

et al. 2015

Expert Review of Molecular Diagnostics

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Non-crosslinking (NCL) approaches using DNA-modified gold nanoparticles for molecular detection constitute powerful tools with potential implications in clinical diagnostics and tailored medicine. From detection of pathogenic agents to identification of specific point mutations associated with health conditions, these methods have shown remarkable versatility and simplicity. Herein, the NCL hybridization assay is broken down to the fundamentals behind its assembly and detection principle. Gold nanoparticle synthesis and derivatization is addressed, emphasizing optimal size homogeneity and conditions for maximum surface coverage, with direct implications in downstream detection. The detection principle is discussed and the advantages and drawbacks of different NCL approaches are discussed. Finally, NCL-based applications for molecular detection of clinically relevant loci and potential integration into more complex biosensing platforms, projecting miniaturization and portability are addressed.

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“…Using a different approach, Baptista and colleagues introduced a noncross-linking method in which a single Au nanoprobe was used in a colorimetric method in order to detect the presence of specific mRNAs from a total RNA extract without retrotranscription or PCR amplification steps (Figure 2) [39,[53][54]. Since then, the versatility of this system has been demonstrated in terms of the RNA detection and quantification of the BCR-ABL fusion gene, which constitutes an important tool in: the early diagnosis of chronic myelogenous leukemia [39]; distinguishing full complementarity from mismatched sequences with a single base mismatch (i.e., to detect common mutations within the β-globin gene) [53]; and a fast and straightforward assay for Mycobacterium tuberculosis DNA detection in clinical samples [54].…”

Section: Dna/rna Aunp-based Molecular Diagnosticsmentioning

confidence: 99%

A Promising Road with Challenges: Where Are Gold Nanoparticles in Translational Research?

Bao

Conde

Polo

et al. 2014

Nanomedicine (Lond.)

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Nanoenabled technology holds great potential for health issues and biological research. Among the numerous inorganic nanoparticles that are available today, gold nanoparticles are fully developed as therapeutic and diagnostic agents both in vitro and in vivo due to their physicochemical properties. Owing to this, substantial work has been conducted in terms of developing biosensors for noninvasive and targeted tumor diagnosis and treatment. Some studies have even expanded into clinical trials. This article focuses on the fundamentals and synthesis of gold nanoparticles, as well as the latest, most promising applications in cancer research, such as molecular diagnostics, immunosensors, surface-enhanced Raman spectroscopy and bioimaging. Challenges to their further translational development are also discussed.

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Bio-microfluidic platform for gold nanoprobe based DNA detection—application to Mycobacterium tuberculosis

Cited by 42 publications

References 32 publications

Gold Nanoparticles for DNA/RNA-Based Diagnostics

Gold Nanoparticles for DNA/RNA-Based Diagnostics

Gold nanoprobe-based non-crosslinking hybridization for molecular diagnostics

A Promising Road with Challenges: Where Are Gold Nanoparticles in Translational Research?

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