Bio-mitigation of carbon dioxide using microalgal systems: Advances and perspectives

Zhou, Wenguang; Wang, Jinghan; Chen, Paul; Ji, Chengcheng; Kang, Qiuyun; Lu, Bei; Li, Kun; Liu, Jin; Ruan, Roger

doi:10.1016/j.rser.2017.03.065

Cited by 265 publications

(104 citation statements)

References 116 publications

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“…Alternative energy sources to petroleum-based fuels have long been pursued, of which biofuels from alga, the next-generation feedstock, have received increasing interest of both academia and industry [1][2][3]. Despite the progresses achieved during the past decades, challenges remain yet to be addressed for bringing down the production cost and realizing commercialization of algal biofuels [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Novel insights into salinity-induced lipogenesis and carotenogenesis in the oleaginous astaxanthin-producing alga Chromochloris zofingiensis: a multi-omics study

Mao

Zhang

Wang

et al. 2020

Biotechnol Biofuels

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Background: Chromochloris zofingiensis, a freshwater alga capable of synthesizing both triacylglycerol (TAG) and astaxanthin, has been receiving increasing attention as a leading candidate producer. While the mechanism of oleaginousness and/or carotenogenesis has been studied under such induction conditions as nitrogen deprivation, high light and glucose feeding, it remains to be elucidated in response to salt stress, a condition critical for reducing freshwater footprint during algal production processes. Results:Firstly, the effect of salt concentrations on growth, lipids and carotenoids was examined for C. zofingiensis, and 0.2 M NaCl demonstrated to be the optimal salt concentration for maximizing both TAG and astaxanthin production. Then, the time-resolved lipid and carotenoid profiles and comparative transcriptomes and metabolomes were generated in response to the optimized salt concentration for congruent analysis. A global response was triggered in C. zofingiensis allowing acclimation to salt stress, including photosynthesis impairment, ROS build-up, protein turnover, starch degradation, and TAG and astaxanthin accumulation. The lipid metabolism involved a set of stimulated biological pathways that contributed to carbon precursors, energy and reductant molecules, pushing and pulling power, and storage sink for TAG accumulation. On the other hand, salt stress suppressed lutein biosynthesis, stimulated astaxanthin biosynthesis (mainly via ketolation), yet had little effect on total carotenoid flux, leading to astaxanthin accumulation at the expense of lutein. Astaxanthin was predominantly esterified and accumulated in a well-coordinated manner with TAG, pointing to the presence of common regulators and potential communication for the two compounds. Furthermore, the comparison between salt stress and nitrogen deprivation conditions revealed distinctions in TAG and astaxanthin biosynthesis as well as critical genes with engineering potential. Conclusions:Our multi-omics data and integrated analysis shed light on the salt acclimation of C. zofingiensis and underlying mechanisms of TAG and astaxanthin biosynthesis, provide engineering implications into future trait improvements, and will benefit the development of this alga for production uses under saline environment, thus reducing the footprint of freshwater.

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Section: Introductionmentioning

confidence: 99%

Novel insights into salinity-induced lipogenesis and carotenogenesis in the oleaginous astaxanthin-producing alga Chromochloris zofingiensis: a multi-omics study

Mao

Zhang

Wang

et al. 2020

Biotechnol Biofuels

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“…Algae‐derived lipids, possessing substantial advantages over plant oils for biofuel production, have been considered as the next‐generation biodiesel feedstock capable of meeting the existing demand for transportation fuels (Chisti, ; Hu et al ., ; Wijffels and Barbosa, ). Past decades have witnessed significant progress in the exploration of algae for biodiesel production, including alga selection, strain trait improvement, development of state‐of‐the‐art cultivation technologies with flue gas and/or wastewater treatment and optimization of downstream processes (Lenka et al ., ; Zhou et al ., ; Raheem et al ., ). Nevertheless, challenges remain yet to be addressed to achieve cost‐effective production of algae‐based biodiesel (Chisti, ; Rodionova et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Multiomics analysis reveals a distinct mechanism of oleaginousness in the emerging model alga Chromochloris zofingiensis

et al. 2019

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Chromochloris zofingiensis, featured due to its capability to simultaneously synthesize triacylglycerol (TAG) and astaxanthin, is emerging as a leading candidate alga for production uses. To better understand the oleaginous mechanism of this alga, we conducted a multiomics analysis by systematically integrating timeresolved transcriptomes, lipidomes and metabolomes in response to nitrogen deprivation. The data analysis unraveled the distinct mechanism of TAG accumulation, which involved coordinated stimulation of multiple biological processes including supply of energy and reductants, carbon reallocation from protein and starch, and 'pushing' and 'pulling' carbon to TAG synthesis. Unlike the model alga Chlamydomonas, de novo fatty acid synthesis in C. zofingiensis was promoted, together with enhanced turnover of both glycolipids and phospholipids, supporting the drastic need of acyls for TAG assembly. Moreover, genomewide analysis identified many key functional enzymes and transcription factors that had engineering potential for TAG modulation. Two genes encoding glycerol-3-phosphate acyltransferase (GPAT), the first committed enzyme for TAG assembly, were found in the C. zofingiensis genome; in vivo functional characterization revealed that extrachloroplastic GPAT instead of chloroplastic GPAT played a central role in TAG synthesis. These findings illuminate distinct oleaginousness mechanisms in C. zofingiensis and pave the way towards rational manipulation of this alga to becone an emerging model for trait improvements.

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“…DIC in aquatic environments can be available as CO 2 , H 2 CO 3 , HCO 3 − , and CO 3 −2 (Ref. ), but CO 2 and HCO 3 − are the only DICs that can be used by microalgae . According to the results presented in Table , it was possible to observe that the initial DIC values did not present significant differences ( p > .05) between the applied experimental conditions.…”

Section: Resultsmentioning

confidence: 87%

“…The CO 2 concentration in flue gases varies depending on the type of fuel in use; for example, it is 5–6% for natural gas and 10–15% for mineral coal . Several CO 2 mitigation strategies have been investigated using physical, chemical, and biological processes . The use of microalgae is a biological strategy, and it is considered a promising alternative to other strategies because of its high photosynthetic efficiency during the bioconversion of CO 2 to biomass compared with terrestrial plants .…”

Section: Introductionmentioning

confidence: 99%

“…3,4 Several CO 2 mitigation strategies have been investigated using physical, chemical, and biological processes. 5 The use of microalgae is a biological strategy, and it is considered a promising alternative to other strategies because of its high photosynthetic efficiency during the bioconversion of CO 2 to biomass compared with terrestrial plants. 6 In addition, the resulting biomass is primarily composed of proteins, carbohydrates, and lipids, which can be used in bioproducts and biofuels such as biodiesel and bioethanol.…”

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confidence: 99%

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Bioprocess strategies for enhancing biomolecules productivity in Chlorella fusca LEB 111 using CO₂ a carbon source

Moraes

Santos

Costa

2019

Biotechnology Progress

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The aim of this study was to evaluate the influence of different carbon dioxide (CO2) concentrations on the distribution of carbon forms in the culture medium and the biomass production and biomolecules productivity of the strain Chlorella fusca LEB 111. In this study, experiments were carried out in which C. fusca cultures were exposed to different CO2 concentrations, 0.03% (0.08 mlCO2 mlmedium−1 days−1), 5% (0.18 mlCO2 mlmedium−1 days−1), and 15% vol/vol CO2 (0.54 mlCO2 mlmedium−1 days−1). Among the carbon chemical species distributions in the culture medium, bicarbonate was predominant (94.2–98.9%), with the highest quantitative percentage in the experiment receiving a 15% CO2 injection. C. fusca LEB 111 cultivated with 15% CO2 showed the highest biomass productivity (194.3 mg L−1 days−1) and CO2 fixation rate (390.9 mg L−1 days−1). The carbohydrate productivity in the culture that received 15% CO2 was 46.2% higher than the value verified for the culture with the addition of CO2 from the air (0.03% CO2). In addition, CO2 concentration providing increases of 0.03–15% to C. fusca cultures resulted in a 31.6% increase in the lipid productivity. These results showed that C. fusca can be used for CO2 bioconversion and for producing biomass with potential applications for biofuels and bioproducts.

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Bio-mitigation of carbon dioxide using microalgal systems: Advances and perspectives

Cited by 265 publications

References 116 publications

Novel insights into salinity-induced lipogenesis and carotenogenesis in the oleaginous astaxanthin-producing alga Chromochloris zofingiensis: a multi-omics study

Novel insights into salinity-induced lipogenesis and carotenogenesis in the oleaginous astaxanthin-producing alga Chromochloris zofingiensis: a multi-omics study

Multiomics analysis reveals a distinct mechanism of oleaginousness in the emerging model alga Chromochloris zofingiensis

Bioprocess strategies for enhancing biomolecules productivity in Chlorella fusca LEB 111 using CO₂ a carbon source

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Bio-mitigation of carbon dioxide using microalgal systems: Advances and perspectives

Cited by 265 publications

References 116 publications

Novel insights into salinity-induced lipogenesis and carotenogenesis in the oleaginous astaxanthin-producing alga Chromochloris zofingiensis: a multi-omics study

Novel insights into salinity-induced lipogenesis and carotenogenesis in the oleaginous astaxanthin-producing alga Chromochloris zofingiensis: a multi-omics study

Multiomics analysis reveals a distinct mechanism of oleaginousness in the emerging model alga Chromochloris zofingiensis

Bioprocess strategies for enhancing biomolecules productivity in Chlorella fusca LEB 111 using CO2 a carbon source

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Product

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Bioprocess strategies for enhancing biomolecules productivity in Chlorella fusca LEB 111 using CO₂ a carbon source