Bio-modulated mice epithelial endometrial organoids by low-level laser therapy serves as an invitro model for endometrial regeneration

Gebril, Mona; AboEl-Maaty, Amal M.; Balah, Osama Al; Taha, Tamer; Abbassy, Amr; Elnoury, Mohamed Amr Hussein

doi:10.1016/j.repbio.2021.100564

Cited by 6 publications

(8 citation statements)

References 28 publications

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“…These treatments have the effect of deeply regenerating and restoring tissue function. Studies show that deep infrared action is capable of increasing the proliferation and functional (functional) cellular capacity of the endometrium [ 41 , 42 , 43 ]. The effect of laser irradiation at the cellular level manifests itself as: increased production of adenosine triphosphate (ATP), increased activity of membrane enzymes, increased synthesis of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), and accelerated electrolyte exchange between the cell and its environment.…”

Section: Physiotherapy In Endometriosismentioning

confidence: 99%

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Physiotherapy Management in Endometriosis

Wójcik¹,

Szczepaniak

Placek

2022

IJERPH

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Endometriosis is a disease whose underlying cause is the growth of the endometrium outside the uterine cavity. The disease is characterised by unpleasant pain in the pelvic region, irrespective of the phase of the woman’s cycle. Physiotherapy in its various forms can be an excellent complement to the gynaecological treatment of endometriosis, by virtue of reducing inflammation, alleviating pain and thus significantly improving women’s quality of life. Physiotherapy in endometriosis should include kinesiotherapy, manual therapy including visceral therapy, physical therapy, spa treatment including balneotherapy, and hydrotherapy. The aim of this study is to present the use of physiotherapy as an adjunct therapy in the treatment of endometriosis. A review of the available literature in the Medline, PubMed and Google Scholar databases was performed without being limited by the time frame of available publications on the forms of physiotherapy used in the treatment of endometriosis.

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Section: Physiotherapy In Endometriosismentioning

confidence: 99%

“…A strong response to pressure may signal adhesions. A complete lack of resistance may indicate uterine retroversion [ 41 , 43 ]. Uterine relaxation techniques are useful in the treatment of reduced uterine mobility.…”

Section: Physiotherapy In Endometriosismentioning

confidence: 99%

Physiotherapy Management in Endometriosis

Wójcik¹,

Szczepaniak

Placek

2022

IJERPH

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“…The effects of PBM on EECs were also confirmed by Gebril in 2021. 11 The pregnancy outcome is the gold standard for verifying endometrial function recovery. 36 In our study, we observed that the pregnancy rate in IUA rats was remarkably higher after PBM.…”

Section: Discussionmentioning

confidence: 99%

“…8,9 Furthermore, the few studies focusing on female reproductive diseases have suggested that PBM increased endometrial cell proliferation and the expression of endometrial receptivity genes in vitro. 10,11 Thus, PBM seems to have great potential for reducing fibrosis and promoting endometrial regeneration. However, whether PBM attenuates endometrial fibrosis and impaired fertility caused by IUA remains unclear.…”

Section: Introductionmentioning

confidence: 99%

“…The potential anti‐fibrotic effects of PBM at various wavelengths on diverse cells and tissues have been reported 8,9 . Furthermore, the few studies focusing on female reproductive diseases have suggested that PBM increased endometrial cell proliferation and the expression of endometrial receptivity genes in vitro 10,11 . Thus, PBM seems to have great potential for reducing fibrosis and promoting endometrial regeneration.…”

Section: Introductionmentioning

confidence: 99%

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Photobiomodulation therapy at 632 nm wavelength ameliorates intrauterine adhesion via activation of cAMP/PKA/CREB pathway

Zheng

Wang

et al. 2023

Photochem & Photobiology

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Intrauterine adhesion (IUA), a major cause of uterine infertility, is pathologically characterized by endometrial fibrosis. Current treatments for IUA have poor efficacy with high recurrence rate, and restoring uterine functions is difficult. We aimed to determine the therapeutic efficacy of photobiomodulation (PBM) therapy on IUA and elucidate its underlying mechanisms. A rat IUA model was established via mechanical injury, and PBM was applied intrauterinely. The uterine structure and function were evaluated using ultrasonography, histology, and fertility tests. PBM therapy induced a thicker, more intact, and less fibrotic endometrium. PBM also partly recovered endometrial receptivity and fertility in IUA rats. A cellular fibrosis model was then established with human endometrial stromal cells (ESCs) cultured in the presence of TGF‐β1. PBM alleviated TGF‐β1‐induced fibrosis and triggered cAMP/PKA/CREB signaling in ESCs. Pretreatment with the inhibitors targeting this pathway weakened PBM's protective efficacy in the IUA rats and ESCs. Therefore, we conclude that PBM improved endometrial fibrosis and fertility via activating cAMP/PKA/CREB signaling in IUA uterus. This study sheds more lights on the efficacy of PBM as a potential treatment for IUA.

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