Bio-nanogate controlled enzymatic reaction for virus sensing

Wang, Ronghui; Xu, Lizhou; Li, Yanbin

doi:10.1016/j.bios.2014.08.071

Cited by 38 publications

(26 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This method not only enables for the visual detection of the virus, but also quantitatively asses the viral load through simple UV-Visual spectroscopy. In another novel study [88], a bio-nanogate comprised of nanoporous (~20 nm) goldfilm attached to H5N1 specific aptamers, was capable of controlling enzymatic reaction for avian influenza virus H5N1 sensing within 1 hour with a detection limit of 2–9HAU (hemagglutination units). It is also interesting to note that no interference was observed from non-target avian influenza virus subtypes such as H1N1, H2N2, H4N8 and H7N2.…”

Section: 3 Studies On Influenza Virusmentioning

confidence: 99%

Role of metal and metal oxide nanoparticles as diagnostic and therapeutic tools for highly prevalent viral infections

Yadavalli

Shukla

2017

Nanomedicine: Nanotechnology, Biology and Medicine

157

111

View full text Add to dashboard Cite

Nanotechnology is increasingly playing important roles in various fields including virology. The emerging use of metal or metal oxide nanoparticles in virus targeting formulations shows the promise of improved diagnostic or therapeutic ability of the agents while uniquely enhancing the prospects of targeted drug delivery. Although a number of nanoparticles varying in composition, size, shape, and surface properties have been approved for human use, the candidates being tested or approved for clinical diagnosis and treatment of viral infections are relatively less in number. Challenges remain in this domain due to a lack of essential knowledge regarding the in vivo comportment of nanoparticles during viral infections. This review provides a broad overview of recent advances in diagnostic, prophylactic and therapeutic applications of metal and metal oxide nanoparticles in Human Immunodeficiency Virus, Hepatitis virus, influenza virus and Herpes virus infections. Types of nanoparticles commonly used and their broad applications have been explained in this review.

show abstract

Section: 3 Studies On Influenza Virusmentioning

confidence: 99%

Role of metal and metal oxide nanoparticles as diagnostic and therapeutic tools for highly prevalent viral infections

Yadavalli

Shukla

2017

Nanomedicine: Nanotechnology, Biology and Medicine

157

111

View full text Add to dashboard Cite

show abstract

“…Virus isolation has been the "gold standard" as it is the highest in sensitivity and reliability, however, it is a very time-consuming procedure (3-7 days). Serological ELISA is a rapid test that can use mass serum samples and is commonly used for serum antibody detection, but it is not used for Various biosensors have been reported, such as surface plasmon resonance (SPR), QCM, fluorescence, optical, imaging ellipsometry, interferometric, and electrochemical [18][19][20][21][22][23][24][25][26][27][28][29][30][31][32].…”

Section: Introductionmentioning

confidence: 99%

A nanowell-based QCM aptasensor for rapid and sensitive detection of avian influenza virus

Wang

Callaway

et al. 2017

Sensors and Actuators B: Chemical

Self Cite

View full text Add to dashboard Cite

In this study, a cost-effective nanowell structure was fabricated and utilized for the development of a nanowell-based quartz crystal microbalance (QCM) aptasensor for rapid, sensitive, and label-free detection of H5N1 avian influenza virus (AIV). A nanoporous gold film with a thickness of 120 nm and a pore size of ~20 nm was prepared using a metallic corrosion method. Then, the nanoporous gold film was immobilized onto a gold electrode surface using a self-assembled monolayer to form a nanowell-based electrode. A specific H5N1 AIV ssDNA aptamer with a NH 2 conjugated 5'-terminal was used in the fabrication of the QCM aptasensor through covalent bonding. The stepwise assembly of the aptasensor was characterized by means of QCM. The result showed that the binding of target AIV H5N1 onto the immobilized aptamers decreased the sensor's resonant frequency, and the frequency change correlated to the virus titer. We demonstrated that the developed nanowell-based QCM aptasensor could dramatically reduce detection time down to 10 min using a label-free assay. The detection range of 2-4 to 2 4 hemagglutination units (HAUs) /50 μl was obtained with a detection limit of 2-4 HAU/50 μl for AIV H5N1. The binding of target H5N1 virus onto the nanowell-based electrode surface was further confirmed by scanning electron microscopy (SEM). No interference was observed from non-target AIV subtypes of H1N1, H2N2, H7N2 and H5N3. The aptasensor using H5N1 aptamer was validated for the detection of AIV H5N1 in chicken tracheal swab samples. The developed aptasensor could be adopted for detection of other viruses.

show abstract

“…A competition-based electrochemical-detection method is the bio-nanogate-based biosensor to measure influenza H5N1, published by Wang et al [79]. Lactate dehydrogenase (LDH) enzymes are coated on a glassy-carbon electrode, which is covered by a gold surface containing bio-nanogates.…”

Section: Electrochemical Aptasensorsmentioning

confidence: 99%

Aptasensors for viral diagnostics

Kieboom

Beek

Mészáros

et al. 2015

TrAC Trends in Analytical Chemistry

View full text Add to dashboard Cite

Novel viral diagnostic tools need to be affordable, fast, accurate and easy to use with sensitivity and specificity equivalent or superior to current standards. At present, viral diagnostics are based on direct detection of viral components or indirect detection by measuring antibodies generated in response to viral infection. While sensitivity of detection and quantification are still important challenges, we expect major advances from new assay formats and synthetic binding molecules, such as aptamers. Compared to traditional antibody-based detection, aptamers could provide faster adaptation to continuously evolving virus strains and higher discriminating capacity between specific virus serotypes. Aptamers are very stable and easily modifiable, so are ideal molecules for detection and chemical sensing applications. Here, we review the use of aptasensors for detection of viral pathogens and consider the feasibility of aptasensors to become standard devices for point-of-care diagnostics of viruses.

show abstract

Bio-nanogate controlled enzymatic reaction for virus sensing

Cited by 38 publications

References 46 publications

Role of metal and metal oxide nanoparticles as diagnostic and therapeutic tools for highly prevalent viral infections

Role of metal and metal oxide nanoparticles as diagnostic and therapeutic tools for highly prevalent viral infections

A nanowell-based QCM aptasensor for rapid and sensitive detection of avian influenza virus

Aptasensors for viral diagnostics

Contact Info

Product

Resources

About