Bio-Preparation and Regulation of Pyrrole Structure Nano-Pigment Based on Biomimetic Membrane

Abstract

Microbial pigments, regarded as the most potential biomass pigments, have lately attracted increasing attention in textile dyeing due to their sustainability and cleaner production. The pyrrole structure microbial pigment, called prodigiosin, recently have become a research hotspot for its bright colors and antibacterial function. However, in most case the extraction and preparation are time-consuming and expensive processes since these kinds of microbial pigments are intracellular metabolites. In order to promote the application of microbial pigments in textile dyeing, a novel idea of preparing dye liquid of pyrrole structure pigments based on fermentation broth was put forward via increasing the proportion of extracellular pigments. A model membrane platform was established with a planar lipid bilayer to investigate transmembrane transport of microbial pigments and permeability barrier of cell membrane. The nano-dispersion of pigments was produced as the dye liquor owing to high-throughput transmembrane transfer of intracellular pigments and the increase of extracellular pigments proportion. The results indicated that the size and surface electrical properties of the pigments had contributed much to the mass transfer. It is also showed that transmembrane transmission of the intracellular pigments could be regulated by physical and chemical methods. With the improvement of transmembrane transfer efficiency of microbial pigments and the proportion of extracellular pigments, the complicated biological separation process could be avoided and the application of microbial pigments in textile dyeing can be promoted.

Production and Potential Applications of Bioconversion of Chitin and Protein-Containing Fishery Byproducts into Prodigiosin: A Review

“…KH-95 [ 206 ], and S. marcescens SMDR [ 207 ] have been studied by adsorption to the materials with hydrophobic surfaces of polyurethane [ 188 ], internal adsorbent using acidified methanol and phase separation [ 206 ], and macroporous polymeric adsorption resin of Diaion HP-20 resins [ 207 ]. The transmembrane transport of PG producing S. marcescens ATCC 8100 and the permeability barrier of the cell membrane were studied by using a model membrane platform with a planar lipid bilayer [ 151 ]. The results showed that the mass transfer of the intracellular PG was affected by its size and surface electrical properties and therefore could be modulated by physical and chemical methods [ 151 ].…”

“…The transmembrane transport of PG producing S. marcescens ATCC 8100 and the permeability barrier of the cell membrane were studied by using a model membrane platform with a planar lipid bilayer [ 151 ]. The results showed that the mass transfer of the intracellular PG was affected by its size and surface electrical properties and therefore could be modulated by physical and chemical methods [ 151 ]. The immobilization strategy to increase PG production was investigated by Chen et al [ 172 ].…”

“…The environmental pollution concerns raised because of the use of chemically synthetic pigment have led to increasing interest in the natural ones. Microbial pigments have lately attracted increasing attention in textile dyeing because of their sustainability and cleaner production [ 75 , 76 , 77 , 78 , 151 , 152 , 169 , 210 ]. Recently, PG has become a research hotspot for its bright colors and antibacterial function.…”

“…Recently, PG has become a research hotspot for its bright colors and antibacterial function. To promote the application of PG in textile dyeing, a novel idea of preparing dye liquid based on fermentation broth was put forward via increasing the proportion of extracellular pigments [ 151 ]. With the improvement in transmembrane transfer efficiency of S. marcescens ATCC8100, PG was produced as the proportion of extracellular pigments, and the complicated biological separation process could be avoided and the application of microbial pigments in textile dyeing can be promoted [ 151 ].…”

Prodigiosin-functionalized Lactobacillus acidophilus ghost: A bioinspired combination against colorectal cancer cells

“…A simple incubation method allowed 3.9% loading of LAGs by varying the composition of a methanolacetic acid solvent system, temperature, incubation time and agitation rate. The solvent system had a key role in PG loading as it was shown to promote permeabilization of the bacterial phospholipid bilayer 37,38 and protonation of PG 39,40 . This would enhance PG binding to the negatively charged LAG membrane via strong electrostatic interaction PG loading which was con rmed by digital and microscopic imaging (Fig.…”