Bio-protective effects of homologous disaccharides on biological macromolecules

Magazù, Salvatore; Migliardo, F.; Benedetto, Antonio; Torre, R. La; Hennet, Louis

doi:10.1007/s00249-011-0760-x

Cited by 31 publications

(23 citation statements)

References 39 publications

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“…our point (c). In the self-distribution function procedure protocol [26][27][28][29][30][31]42], the relationship between the self-distribution function and the MSD is:…”

Section: Concerning Point (C)mentioning

confidence: 99%

“…It is also of interest to examine the normalization procedures most frequently applied both on the measured elastic scattered intensity, and on the extracted MSD [31,42]. It is well known that the measured scattering law, which is a function of Q, represents the number of elastically diffused neutrons within a given solid angle.…”

Section: Concerning Point (C)mentioning

confidence: 99%

“…As a result, starting from the connections between the used instrumental energy resolution with both the measured elastic scattering intensity and the extracted MSD (on this regard, several contributions are reported in literature during the last years [24][25][26][27][28][29][30][31]), it is shown that the observed PDT does not require necessarily any discontinuous change in the temperature behavior of the system relaxation time, and hence it is not necessarily connected to a real transition in the dynamical properties of the system. More specifically, the kink in the measured MSD temperature behavior appears when the system relaxation time crosses the instrumental resolution characteristic time.…”

mentioning

confidence: 94%

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RETRACTED: Protein dynamics by neutron scattering

Benedetto

2013

Biophysical Chemistry

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“…our point (c). In the self-distribution function procedure protocol [26][27][28][29][30][31]42], the relationship between the self-distribution function and the MSD is:…”

Section: Concerning Point (C)mentioning

confidence: 99%

Section: Concerning Point (C)mentioning

confidence: 99%

mentioning

confidence: 94%

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RETRACTED: Protein dynamics by neutron scattering

Benedetto

2013

Biophysical Chemistry

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“…To be effective in preservation, the medium should effectively reduce the local, fast motions which are probably precursors for protein denaturation or other deactivation processes [46]. In this respect, the reduction of water dynamics, as observed in binary systems [47], could be the mechanism for the invoked suppression of short scale motions.…”

Section: T F O R D I S T R I B U T I O Nmentioning

confidence: 99%

Proteins in Saccharides Matrices and the Trehalose Peculiarity: Biochemical and Biophysical Properties

Cordone¹,

Cupane²,

Emanuele

et al. 2015

COC

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Immobilization of proteins and other biomolecules in saccharide matrices leads to a series of peculiar properties that are relevant from the point of view of both biochemistry and biophysics, and have important implications on related fields such as food industry, pharmaceutics, and medicine. In the last years, the properties of biomolecules embedded into glassy matrices and/or highly concentrated solutions of saccharides have been thoroughly investigated, at the molecular level, through in vivo, in vitro, and in silico studies. These systems show an outstanding ability to protect biostructures against stress conditions; various mechanisms appear to be at the basis of such bioprotection, that in the case of some sugars (in particular trehalose) is peculiarly effective.Here we review recent results obtained in our and other laboratories on ternary protein-sugar-water systems that have been typically studied in wide ranges of water content and temperature. Data from a large set of complementary experimental techniques provide a consistent description of structural, dynamical and functional properties of these systems, from atomistic to thermodynamic level.In the emerging picture, the stabilizing effect induced on the encapsulated systems might be attributed to a strong biomolecule-matrix coupling, mediated by extended hydrogen-bond networks, whose specific properties are determined by the saccharide composition and structure, and depend on water content.

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“…Neutron scattering can be used to probe most of these observables. Elastic and quasielastic neutron scattering can access the pico-to nanosecond dynamics (Benedetto and Kearley 2016;Magazù et al 2008Magazù et al , 2009Magazù et al , 2010Magazù et al , 2012Bee 1988;Volino 1978). Neutron spin-echo can be used to investigate slower relaxation processes (Mezei 1972) and to probe structural fluctuations (Nagao 2009;Woodka et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Room-temperature ionic liquids meet bio-membranes: the state-of-the-art

Benedetto

2017

Biophys Rev

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Room-temperature ionic liquids (RTIL) are a new class of organic salts whose melting temperature falls below the conventional limit of 100°C. Their low vapor pressure, moreover, has made these ionic compounds the solvents of choice of the so-called green chemistry. For these and other peculiar characteristics, they are increasingly used in industrial applications. However, studies of their interaction with living organisms have highlighted mild to severe health hazards. Since their cytotoxicity shows a positive correlation with their lipophilicity, several chemical-physical studies of their interactions with biomembranes have been carried out in the last few years, aiming to identify the molecular mechanisms behind their toxicity. Cation chain length and anion nature of RTILs have seemed to affect lipophilicity and, in turn, their toxicity. However, the emerging picture raises new questions, points to the need to assess toxicity on a case-by-case basis, but also suggests a potential positive role of RTILs in pharmacology, bio-medicine and bio-nanotechnology. Here, we review this new subject of research, and comment on the future and the potential importance of this emerging field of study.

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Bio-protective effects of homologous disaccharides on biological macromolecules

Cited by 31 publications

References 39 publications

RETRACTED: Protein dynamics by neutron scattering

RETRACTED: Protein dynamics by neutron scattering

Proteins in Saccharides Matrices and the Trehalose Peculiarity: Biochemical and Biophysical Properties

Room-temperature ionic liquids meet bio-membranes: the state-of-the-art

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