Bio-refinery of xylose processing wastes for green polymalic acid production and l-malic acid recovery by engineered Aureobasidium pullulans in a non-waste-disposal system

Li, Baiyue; Li, Bingqin; Wang, Pan; Yang, Feng; Xu, Xingran; Zhang, Yongjun; Zou, Xiang

doi:10.1016/j.cej.2022.140533

Cited by 11 publications

(4 citation statements)

References 38 publications

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“…Li et al. ( 2023 ) employed adaptive evolution and overexpressed the exogenous CNB gene (calcineurin subunit B) from Beauveria bassiana to obtain an engineered strain AE‐59 of A. pullulans , which synthesized PMA at a concentration of 49 g L −1 at low pH values with Na 2 CO 3 in a 5‐L fermenter using WXML.…”

Section: Polymalatementioning

confidence: 99%

Advances in Aureobasidium research: Paving the path to industrial utilization

Xiao,

Driller,

Dielentheis‐Frenken

et al. 2024

Microbial Biotechnology

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We here explore the potential of the fungal genus Aureobasidium as a prototype for a microbial chassis for industrial biotechnology in the context of a developing circular bioeconomy. The study emphasizes the physiological advantages of Aureobasidium, including its polyextremotolerance, broad substrate spectrum, and diverse product range, making it a promising candidate for cost‐effective and sustainable industrial processes. In the second part, recent advances in genetic tool development, as well as approaches for up‐scaled fermentation, are described. This review adds to the growing body of scientific literature on this remarkable fungus and reveals its potential for future use in the biotechnological industry.

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Section: Polymalatementioning

confidence: 99%

Advances in Aureobasidium research: Paving the path to industrial utilization

Xiao,

Driller,

Dielentheis‐Frenken

et al. 2024

Microbial Biotechnology

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“…Corn cob can be hydrolyzed into corn cob hydrolysate (CCH) with xylose, arabinose and glucose [ 1 , 2 ]. Numerous efforts have been made to obtain efficient routes for resource utilization of CCH [ 3 – 5 ]. Many value-added chemicals including xylitol, [ 6 ] xylonate, [ 7 ] and ethanol [ 8 ] can be produced from CCH by metabolic engineered microorganisms.…”

Section: Introductionmentioning

confidence: 99%

Efficient production of 1,2,4-butanetriol from corn cob hydrolysate by metabolically engineered Escherichia coli

Li,

Wang,

et al. 2024

Microb Cell Fact

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Corn cob is a major waste mass-produced in corn agriculture. Corn cob hydrolysate containing xylose, arabinose, and glucose is the hydrolysis product of corn cob. Herein, a recombinant Escherichia coli strain BT-10 was constructed to transform corn cob hydrolysate into 1,2,4-butanetriol, a platform substance with diversified applications. To eliminate catabolite repression and enhance NADPH supply for alcohol dehydrogenase YqhD catalyzed 1,2,4-butanetriol generation, ptsG encoding glucose transporter EIICBGlc and pgi encoding phosphoglucose isomerase were deleted. With four heterologous enzymes including xylose dehydrogenase, xylonolactonase, xylonate dehydratase, α-ketoacid decarboxylase and endogenous YqhD, E. coli BT-10 can produce 36.63 g/L 1,2,4-butanetriol with a productivity of 1.14 g/[L·h] using xylose as substrate. When corn cob hydrolysate was used as the substrate, 43.4 g/L 1,2,4-butanetriol was generated with a productivity of 1.09 g/[L·h] and a yield of 0.9 mol/mol. With its desirable characteristics, E. coli BT-10 is a promising strain for commercial 1,2,4-butanetriol production.

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“…Biorefineries are complex systems in which lignocellulosic biomass is processed to obtain bioenergy, biofuels, chemicals, and high‐value‐added products 1 . One strategy for biorefineries is to fully convert the sugars remaining from pretreated lignocellulosic biomass after ethanol generation 1,2 .…”

Section: Introductionmentioning

confidence: 99%

“…iorefineries are complex systems in which lignocellulosic biomass is processed to obtain bioenergy, biofuels, chemicals, and high-value-added products. 1 One strategy for biorefineries is to fully convert the sugars remaining from pretreated lignocellulosic biomass after ethanol generation. 1,2 In this way, the carbohydrates not metabolized by ethanol-producing microorganisms can be used to produce other chemicals of industrial interest, such as acetic acid (AA) and lactic acid (LA).…”

mentioning

confidence: 99%

The use of xylose from elephant grass liquor and coproduction of organic acids by wild‐type bacteria isolated from food waste

Menegussi,

Montipó,

Fontana

et al. 2023

Biofuels Bioprod Bioref

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During the normal production of ethanol from pretreated lignocellulosic biomass, only the solid fraction containing C6 sugars is generally used by unmodified yeasts in the fermentation process – the remaining liquid fraction, rich in xylose, is used almost exclusively by genetically modified organisms (GMOs) or for other purposes. As the liquor can be used in the manufacture of a series of chemical inputs, microorganisms that metabolize C5 sugars from different sources were initially isolated with the aim of converting these sugars into acetic acid (AA) and lactic acid (LA). Experiments were carried out with pretreated elephant grass liquor, evaluating the effect of adding commonly referenced nutrients through experimental design. Thus, the present work involves the isolation, screening, and molecular characterization of bacteria, easily found in nature, with the potential to metabolize xylose and produce AA and LA, with yields similar or superior to those shown in studies employing GMOs. Using wild‐type strains, high yields were obtained with Acetobacter cerevisiae (0.96 g LA and 0.71 g AA per g total sugars) and Levilactobacillus brevis (0.77 g LA and 0.61 g AA per g total sugars) in relation to the organic acids of interest, highlighting the total conversion of the sugar blend (xylose, glucose, and arabinose) in 24 h. Ammonium citrate also significantly influenced the final yields, being responsible for the increase in production. The results are useful for biorefinery platforms and may contribute to the full use of sugars released in the lignocellulosic pretreatment of raw materials.

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Bio-refinery of xylose processing wastes for green polymalic acid production and l-malic acid recovery by engineered Aureobasidium pullulans in a non-waste-disposal system

Cited by 11 publications

References 38 publications

Advances in Aureobasidium research: Paving the path to industrial utilization

Advances in Aureobasidium research: Paving the path to industrial utilization

Efficient production of 1,2,4-butanetriol from corn cob hydrolysate by metabolically engineered Escherichia coli

The use of xylose from elephant grass liquor and coproduction of organic acids by wild‐type bacteria isolated from food waste

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