Bio Remediation of CO2 and Characterization of Carbonic Anhydrase 
 from Mangrove Bacteria

Bhagat, Chintan; Tank, Shantilal K.; Ghelani, Anjana; Dudhagara, Pravin; Patel, Rajesh

doi:10.3923/jest.2014.76.83

Cited by 9 publications

(4 citation statements)

References 14 publications

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“…The input of carbonic anhydrase (EC 4.2.2.1) (a zinc metalloenzyme biocatalyst) catalyzes the reversible hydration of CO 2 (CO 2 + H 2 O  H + + HCO 3 -), which is responsible for speeding up the MC. In search of suitable CA, numerous microbial CAs from different habitats were screened and engineered for carbonate production to sequester CO 2 [4,[8][9][10][11]. Kupriyanova et al, (2007) demonstrated the role of inorganic carbon in a CaCO 3 deposition in the mineralization process by Microcoleus chthonoplastes which significantly enhanced the formation of CaCO 3 and acted as an intimation for the growth of mineralization for CO 2 sequestration [12].…”

Section: Introductionmentioning

confidence: 99%

Effect of Different Calcium Salts on Calcium Carbonates Formation Induced by Halophilic Bacillus oceanisediminis CB1

et al. 2022

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Biomineralization through the biomimetic CO2 sequestration process has been gaining attraction in recent years due to the formation of carbonates widely used as raw material in various industrial processes. The deposition and dissolution of calcium carbonate can be affected by physiochemical factors, such as the type of calcium salt. However, most studies have focused on calcium chloride (CaCl2). In the present study, A potent bacterial carbonic anhydrase (CA) producer, Bacillus oceanisediminis CB1, was screened on CA activity from mangrove plant Avicennia marina, collected from Ghogha, Bhavnagar, India (21.68°N 72.28°E). We premeditated deposition experiments to determine the effects of different calcium salts on calcium carbonate deposition in Bacillus oceanisediminis CB1 colonies. The results demonstrated the calcite formation observed in calcium salt-supplemented nutrient agar, calcium chloride, and calcium acetate. Merely uniform distribution and peripheral distribution of calcite particles found in calcium acetate and calcium chloride supplemented into nutrient agar, respectively. Calcite formation was confirmed by staining with Alizarin Red S dye followed by SEM-EDX. This study will provide a vital reference for designing and applying microbial-induced carbonate precipitation using different calcium salts.

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Section: Introductionmentioning

confidence: 99%

Effect of Different Calcium Salts on Calcium Carbonates Formation Induced by Halophilic Bacillus oceanisediminis CB1

et al. 2022

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“…, ; Bhagat et al . ; Ghelani et al . ; Okyay and Rodrigues ; Faridi and Satyanarayana ; Okyay et al .…”

Section: Introductionmentioning

confidence: 99%

“…Further, it appears to be an eco-friendly, feasible, environment benign and viable technology that permits CCUS for both, large and medium emitters on site. Therefore, CAs have been investigated by different researchers to discover feasible and viable CA for the mineralization process (Favre et al 2009;Sanna et al 2012Sanna et al , 2014Bhagat et al 2014;Ghelani et al 2015;Okyay and Rodrigues 2015;Faridi and Satyanarayana 2016;Okyay et al 2016). However, the low stability of CAs under various industrial conditions is a major problem.…”

Section: Introductionmentioning

confidence: 99%

Trends, application and future prospectives of microbial carbonic anhydrase mediated carbonation process for CCUS

2017

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Summary Growing industrialization and the desire for a better economy in countries has accelerated the emission of greenhouse gases (GHGs), by more than the buffering capacity of the earth's atmosphere. Among the various GHGs, carbon dioxide occupies the first position in the anthroposphere and has detrimental effects on the ecosystem. For decarbonization, several non‐biological methods of carbon capture, utilization and storage (CCUS) have been in use for the past few decades, but they are suffering from narrow applicability. Recently, CO2 emission and its disposal related problems have encouraged the implementation of bioprocessing to achieve a zero waste economy for a sustainable environment. Microbial carbonic anhydrase (CA) catalyses reversible CO2 hydration and forms metal carbonates that mimic the natural phenomenon of weathering/carbonation and is gaining merit for CCUS. Thus, the diversity and specificity of CAs from different micro‐organisms could be explored for CCUS. In the literature, more than 50 different microbial CAs have been explored for mineral carbonation. Further, microbial CAs can be engineered for the mineral carbonation process to develop new technology. CA driven carbonation is encouraging due to its large storage capacity and favourable chemistry, allowing site‐specific sequestration and reusable product formation for other industries. Moreover, carbonation based CCUS holds five‐fold more sequestration capacity over the next 100 years. Thus, it is an eco‐friendly, feasible, viable option and believed to be the impending technology for CCUS. Here, we attempt to examine the distribution of various types of microbial CAs with their potential applications and future direction for carbon capture. Although there are few key challenges in bio‐based technology, they need to be addressed in order to commercialize the technology.

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“…So CA catalyzes the hydration reaction at or near the diffusion-controlled limit that the formation of product is much faster than the dissemination of reactants 4 . Carbonic anhydrase producing bacteria were reported from various habitats, including sediments, deep seawater, mangroves soil, karst soil and alkaline soil [8][9][10][11] . Nocardiopsis lucentensis type strain DSM 44048 was the first time isolated from a salt marsh soil sample near Alicante, Spain in 1993 12 .…”

Section: Introductionmentioning

confidence: 99%

Biomimetic Sequestration of CO2 Using Carbonic Anhydrase from Calcite Encrust Forming Marine Actinomycetes

Ghelani¹,

Bhagat²,

Dudhagara³

et al. 2015

Science International

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Background: CO 2 in the environment has been a burning issue and aggravated the threats of global warming. Various strategies are being used to decrease the level of releasing CO 2 . Biosequestration is a one of the available nontoxic, robust and eco-friendly approaches but still less evaluated. This study reports the role of microbial Carbonic Anhydrase (CA) in the formation of calcite by utilizing CO 2 . Methods: The marine Nocardiopsis lucentensis was studied for intracellular microbial CA. Isolate grown optimally at 2.5% CO 2 saturated environment and procedure the calcium carbonate encrust in the presence of CaCl 2 . Results: The enzyme was found to catalyze the reaction in a wide range of pH and temperature with an optimum at 7.0 pH and 25°C. The 50 mM NaCl, KCl and MgCl 2 were found to support the enzyme activity and 50 mM ZnCl 2 increases activity 1.5 fold. CA was able to withstand against sulphanilamide inhibitor and stable over prolonged incubation at 4 and 37°C. Conclusion: Calcite formation was evaluated with and without enzyme using marine water as a source of calcium ions. The result of SEM and EDX indicated the formation of larger flower-shaped particles compare to the small cubic particles formed without enzyme. The results suggest that the robustness of enzyme and suitability in the CO 2 capture reactor.

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Bio Remediation of CO2 and Characterization of Carbonic Anhydrase from Mangrove Bacteria

Cited by 9 publications

References 14 publications

Effect of Different Calcium Salts on Calcium Carbonates Formation Induced by Halophilic Bacillus oceanisediminis CB1

Effect of Different Calcium Salts on Calcium Carbonates Formation Induced by Halophilic Bacillus oceanisediminis CB1

Trends, application and future prospectives of microbial carbonic anhydrase mediated carbonation process for CCUS

Biomimetic Sequestration of CO2 Using Carbonic Anhydrase from Calcite Encrust Forming Marine Actinomycetes

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