Bio-sample detection on paper-based devices with inkjet printer-sprayed reagents

Liang, Wun Hong; Chu, Chien Hung; Yang, Ruey-Jen

doi:10.1016/j.talanta.2015.06.029

Cited by 11 publications

(4 citation statements)

References 30 publications

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“…Thus, microfluidic paper-based analytical devices (µPADs) have been widely applied in such fields as medicine [6][7][8], the food industry [9][10][11], and environmental fields [12][13][14]. Multiple processes can be applied to create a hydrophobic barrier on paper, including photolithography [15,16], light irradiation [17], plasma treatment [18], laser cutting [19], stamping [20], inkjet printing [21,22], wax printing [23,24], and screen printing [25,26]. However, photolithography, plasma-treatment, laser-cutting, and inkjet-printing techniques have some limitations, such as expensive fabrication instrumentation, multi-step fabrication processes, and long fabrication times [27].…”

Section: Introductionmentioning

confidence: 99%

One-Step Polylactic Acid Screen-Printing Microfluidic Paper-Based Analytical Device: Application for Simultaneous Detection of Nitrite and Nitrate in Food Samples

2019

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In this work, we report a one-step approach for fabricating screened-printed microfluidic paper-based analytical devices (μPADs) using polylactic acid as a new hydrophobic material. A polylactic acid solution was screen printed onto chromatography papers to create hydrophobic patterns for fluidic channels. The optimal polylactic acid concentration for successful device fabrication is 9% w/v. The μPADs were fabricated within 2 min and provided high reproducibility and stability. The utility of polylactic acid screen-printing was demonstrated for the simultaneous detection of nitrite and nitrate using colorimetric detection. Under optimized experimental conditions, the detection limits and the linear ranges, respectively, were 1.2 mg L−1 and 2–10 mg L−1 for nitrite and 3.6 mg L−1 and 10–50 mg L−1 for nitrate. The detection times for both ions were found to be within 12 min. The developed μPAD was applied for the simultaneous determination of these ions in food samples, and no significant differences in the analytical results were observed compared to those of the reference method. The polylactic acid screen-printing approach presented here provides a simple, rapid, and cost-effective alternative fabrication method for fabricating μPADs.

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Section: Introductionmentioning

confidence: 99%

One-Step Polylactic Acid Screen-Printing Microfluidic Paper-Based Analytical Device: Application for Simultaneous Detection of Nitrite and Nitrate in Food Samples

2019

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“…The RGB intensities, 1-10 the RGB absorbance (A R , A G , A B , A RGB ), [11][12][13][14] the normalized I G /I R ratio, 15 the cyan, magenta, and yellow (CMY) intensities, [16][17][18][19] the tristimulus XYZ parameters, [20][21][22] the greyscale intensity, 23,24 and the inverted 8 bit greyscale intensity 25,26 have been used as analytical signals in DIBA. Images have been captured using digital cameras, 9,14,18,24 mobile phone cameras, 4,7,12,15,17,19,23 web cams, 10,11 or computer scanners. 6,13,16,17,25,26 However, some other excellent papers on the topic have been undoubtedly missed and can be found in excellent reviews of the topic.…”

Section: Introductionmentioning

confidence: 99%

The Yxy colour space parameters as novel signalling tools for digital imaging sensors in the analytical laboratory

2018

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Digital imaging devices can be promising, sensitive, and cost-effective chemical sensors for resourcelimited settings. Three model colour reactions of iron were used and monitored using a simple platform consisting only of a camera, a cuvette, and a white paper diffuser. A desktop scanner and a mobile phone camera were also used as imaging devices. Captured images were analysed to obtain the RGB intensities (red, green, blue) and were further converted into the corresponding signals of the grayscale,

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“…Inkjet printing has many advantages for PAD manufacturing, including a rapid throughput, low cost, a straightforward process, good versatility, and the potential for mass production. Moreover, the cartridges of the printer can be filled with different reagents in order to realize multi-assay devices in a single printing run [ 28 , 29 ]. The present study uses a modified inkjet printer to spray biochemical reagents onto PADs for the detection of AST and ALT.…”

Section: Introductionmentioning

confidence: 99%

Aspartate Aminotransferase and Alanine Aminotransferase Detection on Paper-Based Analytical Devices with Inkjet Printer-Sprayed Reagents

et al. 2016

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General biochemistry detection on paper-based microanalytical devices (PADs) uses pipette titration. However, such an approach is extremely time-consuming for large-scale detection processes. Furthermore, while automated methods are available for increasing the efficiency of large-scale PAD production, the related equipment is very expensive. Accordingly, this study proposes a low-cost method for PAD manufacture, in which the reagent is applied using a modified inkjet printer. The optimal reaction times for the detection of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) are shown to be 6 and 7 min, respectively, given AST and ALT concentrations in the range of 5.4 to 91.2 U/L (R2 = 0.9932) and 5.38 to 86.1 U/L (R2 = 0.9944). The experimental results obtained using the proposed PADs for the concentration detection of AST and ALT in real human blood serum samples are found to be in good agreement with those obtained using a traditional spectrophotometric detection method by National Cheng Kung University hospital.

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Bio-sample detection on paper-based devices with inkjet printer-sprayed reagents

Cited by 11 publications

References 30 publications

One-Step Polylactic Acid Screen-Printing Microfluidic Paper-Based Analytical Device: Application for Simultaneous Detection of Nitrite and Nitrate in Food Samples

One-Step Polylactic Acid Screen-Printing Microfluidic Paper-Based Analytical Device: Application for Simultaneous Detection of Nitrite and Nitrate in Food Samples

The Yxy colour space parameters as novel signalling tools for digital imaging sensors in the analytical laboratory

Aspartate Aminotransferase and Alanine Aminotransferase Detection on Paper-Based Analytical Devices with Inkjet Printer-Sprayed Reagents

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