Bio-signature of Ultraviolet-Radiation-Resistant Extremophiles from Elevated Land

Gabani, Prashant; Prakash, Dhan; Singh, Om V.

doi:10.12691/ajmr-2-3-3

Cited by 8 publications

(8 citation statements)

References 45 publications

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“…The rod-shaped strain HC41 T was observed using field-emission scanning electron microscopy (Quanta 250 FEG). Catalase and oxidase activities were assessed by adding 10 µl droplets of 3% (w/v) hydrogen peroxide solution and 1 % (w/v) N , N , N′ , N′ -tetramethyl- p -phenylenediamine dihydrochloride onto the colonies, respectively [25, 26]. The occurrence of bubbles and a violet coloration within 5 min were indicative of catalase and oxidase activity, respectively.…”

Section: Physiology and Chemotaxonomymentioning

confidence: 99%

Niveibacterium microcysteis sp. nov., isolated from a cyanobacterial bloom sample

Kang

Park

2021

International Journal of Systematic and Evolutionary Microbiology

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A novel bacterial strain, named HC41T, was isolated from a cyanobacterial bloom sample and was characterized as Gram-stain-negative, rod-shaped and non-motile. According to 16S rRNA phylogenetic analyses, this strain HC41T belongs to the family Rhodocyclaceae and is most closely related to Niveibacterium umoris KACC 17062T (=MIC 2059T; 98.63 %) and Uliginosibacterium gangwonense 5YN10-9 T (=KACC 11603T; 93.64 %). The genome size and DNA G+C content of strain HC41T were 4.8 Mbp and 64.17 mol%, respectively. Moreover, the average nucleotide identity, digital DNA–DNA hybridization and amino acid identity values between strain HC41T and N. umoris KACC 17062T were 81.8, 43.1 and 90.89 %, respectively. Additionally, strain HC41T exhibited weak catalase and oxidase activities and had no motility (swimming and swarming motilities). The cells grew at 11–40 °C (optimum, 30 °C), pH 5.5–8.0 (optimum, pH 7) and with 0–1.0 % (w/v) NaCl (optimum, 0 % NaCl) in Reasoner’s 2A medium. Its major respiratory quinone was ubiquinone-8 and its major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Furthermore, C16 : 0 and summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c; C16 : 1 ω6c and/or C16 : 1 ω7c) were the predominant cellular fatty acids in strain HC41T according to fatty acid methyl ester analysis. Based on its genotypic and phenotypic characteristics, strain HC41T was identified as representing a novel Niveibacterium species, for which the name Niveibacterium microcysteis sp. nov. is proposed (=KACC 22091T=DSM 111425T).

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Section: Physiology and Chemotaxonomymentioning

confidence: 99%

Niveibacterium microcysteis sp. nov., isolated from a cyanobacterial bloom sample

Kang

Park

2021

International Journal of Systematic and Evolutionary Microbiology

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“…Atacama Desert is characterized by its high altitude, prevalent cloudless conditions, extreme dryness, relatively low columns of ozone and water vapor and intense solar UV radiation ( Cordero et al, 2016 ; Paulino-Lima et al, 2016 ), which converts this desert in a key region for international astrobiology studies. The microorganisms that are capable of living under these extreme conditions are known as UV resistant extremophiles ( Gabani et al, 2014 ). The study of microorganisms living in these hostile environments is currently focused to gain understanding on the origin of life and early evolution on Earth, to develop models for predicting consequences of future Climate change and to exploit their potential for biotechnological applications ( Pérez et al, 2017 ).…”

Section: Uv- and Gamma-resistant Microorganismsmentioning

confidence: 99%

“…Extremophiles, can be classified according to the conditions in which they grow: thermophiles and hyperthermophiles (organisms growing at temperatures of 45–80°C and >80°C, respectively) ( Madigan et al, 2000 ; Berenguer, 2011 ), psychrophiles (organisms that grow at <10°C) ( Siddiqui et al, 2013 ), acidophiles and alkaliphiles (organisms optimally adapted to pH < 5 and pH > 9, respectively), halophiles (organisms that require NaCl for growth, in concentration of 200–5,900 mM) ( Edbeib et al, 2016 ), microorganisms that survive in dry environments (water activity < 0.75) ( Connon et al, 2007 ) and radiotolerant (UV resistant) extremophiles that are resistant to the permanent exposure to damaging solar radiation ( Gabani et al, 2014 ) (Table 1 ). Additionally, it is worth mentioning that extremophiles are usually defined by one extreme condition, nevertheless, many natural environments possess two or more extreme conditions.…”

Section: Introductionmentioning

confidence: 99%

Living at the Frontiers of Life: Extremophiles in Chile and Their Potential for Bioremediation

et al. 2018

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Extremophiles are organisms capable of adjust, survive or thrive in hostile habitats that were previously thought to be adverse or lethal for life. Chile gathers a wide range of extreme environments: salars, geothermal springs, and geysers located at Altiplano and Atacama Desert, salars and cold mountains in Central Chile, and ice fields, cold lakes and fjords, and geothermal sites in Patagonia and Antarctica. The aims of this review are to describe extremophiles that inhabit main extreme biotopes in Chile, and their molecular and physiological capabilities that may be advantageous for bioremediation processes. After briefly describing the main ecological niches of extremophiles along Chilean territory, this review is focused on the microbial diversity and composition of these biotopes microbiomes. Extremophiles have been isolated in diverse zones in Chile that possess extreme conditions such as Altiplano, Atacama Desert, Central Chile, Patagonia, and Antarctica. Interesting extremophiles from Chile with potential biotechnological applications include thermophiles (e.g., Methanofollis tationis from Tatio Geyser), acidophiles (e.g., Acidithiobacillus ferrooxidans, Leptospirillum ferriphilum from Atacama Desert and Central Chile copper ores), halophiles (e.g., Shewanella sp. Asc-3 from Altiplano, Streptomyces sp. HKF-8 from Patagonia), alkaliphiles (Exiguobacterium sp. SH31 from Altiplano), xerotolerant bacteria (S. atacamensis from Atacama Desert), UV- and Gamma-resistant bacteria (Deinococcus peraridilitoris from Atacama Desert) and psychrophiles (e.g., Pseudomonas putida ATH-43 from Antarctica). The molecular and physiological properties of diverse extremophiles from Chile and their application in bioremediation or waste treatments are further discussed. Interestingly, the remarkable adaptative capabilities of extremophiles convert them into an attractive source of catalysts for bioremediation and industrial processes.

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“…So, it is necessary to investigate the diversity of ultraviolet radiation resistant microorganisms from such environments in order to study in future the physiological mechanisms providing survival of microorganisms under the pressure of ultraviolet radiation (Gabani et al, 2014). We did not find data on the UV resistance of microorganisms of investigated ecosystems in the literature available for us.…”

Section: Introductionmentioning

confidence: 96%

“…The ability of microorganisms to survive at high levels of UV irradiation is associated with the stability of their genome (Goosen et al, 2008;Singh et al, 2011). Bacteria resistant to UV radiation have been reported (Singh et al, 2011;Gabani et al, 2014) to be capable to produce the metabolites of the primary and secondary metabolism for their protection. According to the literature (Sinha et al, 1998), cyanobacteria have developed mechanisms for counteraction to UV radiation.…”

Section: Introductionmentioning

confidence: 99%

Correlation Between Resistance to UV Irradiation and the Taxonomic Position of Microorganisms

Gladka

Hovorukha²,

Romanovskaya³

et al. 2021

EREM

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UV irradiation is known to cause harmful effects on microorganisms. As the result, microorganisms have developed protection against exposure to harmful UV irradiation. We suggested that resistance to UV might be specific characteristic of certain genera of microorganisms. So, the aim of the work is to assess whether there is a correlation between UV resistance and the taxonomic position of microorganisms or is it the adaptation of cells to extreme conditions. There were studied 67 strains from extreme ecosystems (phytocenoses and оrnithogenic soil of the Antarctic, hypersaline ecosystems of the Crimea and the Dead Sea, the lake Baikal). Sequence of 16S rRNA genes, phylogenetic analysis and UV resistance were performed according to standard procedures. Phylogenetic analysis revealed live representatives of the following genera: Pseudomonas, Serratia, Rheinheimera, Aeromonas, Buttiauxella, Brevundimonas, Sphingomonas, Sphingopyxis, Dermacoccus, Frondihabitans, Microbacterium, Rhodococcus, Janthinobacterium, Arthrobacter, Micrococcus, Kocuria, Sphingobacterium, Flavobacterium, Chryseobacterium, Bacillus, Staphylococcus, Paenibacillus, Sporosarcina. Gram-positive bacteria were significantly more resistant to UV irradiation (LD99.99 750–1400 J/m2). Gram-negative had lower resistance (LD99.99 35–150 J/m2). Most pigmented strains were more resistant to UV than non-pigmented ones. According to the values of lethal doses of UV irradiation we suggest that investigated microorganisms have effective mechanisms to repair DNA damages. Resistance of microorganisms to UV, as a rule, was not related to the ecological features of their habitat. Thus, there is a correlation between the resistance of microorganisms to UV irradiation and their taxonomic position, which allows to consider UV resistance as a diagnostic feature at the genus level.

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Bio-signature of Ultraviolet-Radiation-Resistant Extremophiles from Elevated Land

Cited by 8 publications

References 45 publications

Niveibacterium microcysteis sp. nov., isolated from a cyanobacterial bloom sample

Niveibacterium microcysteis sp. nov., isolated from a cyanobacterial bloom sample

Living at the Frontiers of Life: Extremophiles in Chile and Their Potential for Bioremediation

Correlation Between Resistance to UV Irradiation and the Taxonomic Position of Microorganisms

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