Bio-waste derived dialdehyde cellulose ethers as supports for α-chymotrypsin immobilization

Kumari, Sapana; Chauhan, Ghanshyam S.; Ahn, Jou–Hyeon; Reddy, N.S.

doi:10.1016/j.ijbiomac.2015.12.063

Cited by 20 publications

(1 citation statement)

References 43 publications

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“…The presence of hydroxyl moieties in cellulose can be used to add functional groups able to make the immobilization of GOx feasible. , Periodate mediated cellulose oxidation generates aldehyde moieties that can be used to bind the enzyme lysines via imine formation, also known as a Schiff base . This well-established binding method has been used for the immobilization of proteins and enzymes on cellulose-based materials. − The general approach to the creation of the GOx-functionalized beads herein used is shown in Scheme . Details are provided in the Experimental Section below.…”

Section: Introductionmentioning

confidence: 92%

Enzyme-Functionalized Cellulose Beads as a Promising Antimicrobial Material

et al. 2021

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The extensive use of antibiotics over the last decades is responsible for the emergence of multidrug-resistant (MDR) microorganisms that are challenging health care systems worldwide. The use of alternative antimicrobial materials could mitigate the selection of new MDR strains by reducing antibiotic overuse. This paper describes the design of enzyme-based antimicrobial cellulose beads containing a covalently coupled glucose oxidase from Aspergillus niger (GOx) able to release antimicrobial concentrations of hydrogen peroxide (H 2 O 2 ) (≈ 1.8 mM). The material preparation was optimized to obtain the best performance in terms of mechanical resistance, shelf life, and H 2 O 2 production. As a proof of concept, agar inhibition halo assays (Kirby-Bauer test) against model pathogens were performed. The two most relevant factors affecting the bead functionalization process were the degree of oxidation and the pH used for the enzyme binding process. Slightly acidic conditions during the functionalization process (pH 6) showed the best results for the GOx/cellulose system. The functionalized beads inhibited the growth of all the microorganisms assayed, confirming the release of sufficient antimicrobial levels of H 2 O 2 . The maximum inhibition efficiency was exhibited toward Pseudomonas aeruginosa ( P. aeruginosa ) and Escherichia coli ( E. coli ), although significant inhibitory effects toward methicillin-resistant Staphylococcus aureus (MRSA) and S. aureus were also observed. These enzyme-functionalized cellulose beads represent an inexpensive, sustainable, and biocompatible antimicrobial material with potential use in many applications, including the manufacturing of biomedical products and additives for food preservation.

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Section: Introductionmentioning

confidence: 92%

Enzyme-Functionalized Cellulose Beads as a Promising Antimicrobial Material

et al. 2021

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Utilization of Biowastes in Green Chemistry

Deka¹,

Gohain²,

Bhuyan³

et al. 2022

Climate Change and Agriculture

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Correlating the 3D melt electrospun polycaprolactone fiber diameter and process parameters using neural networks

Narayana

Wang

Yeom

et al. 2021

J of Applied Polymer Sci

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In the present work, we developed an artificial neural networks (ANN) model to predict and analyze the polycaprolactone fiber diameter as a function of 3D melt electrospinning process parameters. A total of 35 datasets having various combinations of electrospinning writing process variables (collector speed, tip to nozzle distance, applied pressure, and voltage) and resultant fiber diameter were considered for model development. The designed stand‐alone ANN software extracts relationships between the process variables and fiber diameter in a 3D melt electrospinning system. The developed model could predict the fiber diameter with reasonable accuracy for both train (28) and test (7) datasets. The relative index of importance revealed the significance of process variables on the fiber diameter. Virtual melt spinning system with the mean values of the process variables identifies the quantitative relationship between the fiber diameter and process variables.

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Bio-waste derived dialdehyde cellulose ethers as supports for α-chymotrypsin immobilization

Cited by 20 publications

References 43 publications

Enzyme-Functionalized Cellulose Beads as a Promising Antimicrobial Material

Enzyme-Functionalized Cellulose Beads as a Promising Antimicrobial Material

Utilization of Biowastes in Green Chemistry

Correlating the 3D melt electrospun polycaprolactone fiber diameter and process parameters using neural networks

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