Bioaccumulation characterization of cadmium by growing Bacillus cereus RC-1 and its mechanism

Huang, Fei; Guo, Chuling; Lu, Guining; Yi, Xiaoyun; Zhu, Linna; Dang, Zhi

doi:10.1016/j.chemosphere.2014.01.066

Cited by 125 publications

(54 citation statements)

References 47 publications

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“…Removal of Cd +2 contaminants from the environment by using microbial biomass has been reported in several investigations (Yan and Viraraghavan 2003;Feng and Aldrich 2004;Vijayaraghavan and Yun 2008;He et al 2011;Huang et al 2014). Bacteria have great potential to remove heavy metal ions from the environment.…”

Section: Discussionmentioning

confidence: 98%

“…Bioremediation of metal ions by using microbial biomass of bacteria (Vijayaraghavan and Yun 2008), algae (Feng and Aldrich 2004) and fungi (Yan and Viraraghavan 2003) is one of the most popular strategies due to environmental friendliness, cost effectiveness, and ability to work in low concentrations of metal ions (He et al 2011;Huang et al 2014). Some investigations have shown that bacterial strains, either dead or living form, have ability to accumulate cadmium ions (El-Helow et al 2000;Zouboulis et al 2004;Huang et al 2013).…”

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Cadmium resistance mechanism in Escherichia coli P4 and its potential use to bioremediate environmental cadmium

Khan

Nisar

Hussain

et al. 2015

Appl Microbiol Biotechnol

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A cadmium-resistant bacterium was isolated from industrial wastewater and identified as Escherichia coli (dubbed as P4) on the basis of morphological, biochemical tests and 16S rRNA ribotyping. It showed optimum growth at 30 °C and pH 7. E. coli P4 found to resist Cd(+2) (10.6 mM) as well as Zn(+2) (4.4 mM), Pb(+2) (17 mM), Cu(+2) (3.5 mM), Cr(+6) (4.4 mM), As(+2) (10.6 mM), and Hg(+2) (0.53 mM). It could remove 18.8, 37, and 56 % Cd(+2) from aqueous medium after 48, 96, and 144 h, respectively. Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), and Energy-dispersive X-ray (EDX) analysis also confirmed the biosorption of Cd(+2) by E. coli P4. However, temperature and pH were found to be the most critical factors in biosorption of Cd(+2) by E. coli P4. Cd(+2) stress altered E. coli P4 cell physiology analyzed by measuring glutathione (GSH) and non-protein thiol (cysteine) levels which were increased up to 130 and 48 %, respectively. Quantitative real-time polymerase chain reaction (qRT-PCR) showed alteration in the expression levels of ftsZ, mutS, clpB, ef-tu, and dnaK genes in the presence of Cd(+2). Total protein profiles of E. coli P4 in the absence and presence of Cd(+2) were compared by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), which showed remarkable difference in the banding pattern. czcB gene, a component of czcCBA operon, was amplified from genomic DNA which suggested the chromosomal-borne Cd(+2) resistance in E. coli P4. Furthermore, it harbors smtAB gene which plays a significant role in Cd(+2) resistance.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 98%

Cadmium resistance mechanism in Escherichia coli P4 and its potential use to bioremediate environmental cadmium

Khan

Nisar

Hussain

et al. 2015

Appl Microbiol Biotechnol

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“…Additionally, intracellular accumulation is a more complex process and may involve localization of the metal within specific subcellular fractions (Desaunay and Martins 2014); but these mechanisms are still not well understood, especially in Gram-positive bacteria. Within their impressive physiological diversity, members of the genus Bacillus perform toxic-metal bioaccumulation by way of metabolism-independent extracellular adsorption, that is: the surface interaction of heavy-metal ions with cell-surface components such as S-layer proteins (Guo et al 2010;Huang et al 2014). Additionally, it has been reported efflux pumps in Gram-positive bacteria in targets like antibiotics, drugs (Masaoka et al 2000), and heavy metals such as Zn, Cd, Co, Ni, and Ag (Gaballa and Helmann 2003;Moore and Helmann 2005;Kihlken et al 2008), but they have never been reported in metals associated with crude oil like Cr, Pb, and As (Stigter et al 2000;Duyck et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Mathematical Modelling of Toxic Metal Uptake and Efflux Pump in Metal-Resistant Bacterium Bacillus cereus Isolated From Heavy Crude Oil

Shaw

Dussán

2015

Water Air Soil Pollut

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The aim of this study was to describe the mechanisms that native Bacillus cereus M6 isolated from heavy crude oil o API gravity 11.5 uses to tolerate and/or resist toxic metals. Metal tolerance and removal of Pb(II), Cr(VI), and As(V) was determined. In addition, we evidenced the subcellular distribution of metals, the efflux pump kinetics, and morphological changes in metal-tolerant bacteria. B. cereus M6 exhibited strong tolerance and resistance to the metals evaluated and efficiently removed the metal content by operating efflux pumps and accumulating mainly in membrane fraction. Also, it was found that the model that best fit the efflux corresponds to an equation for resonant oscillations. B. cereus M6 uses mechanisms, including efflux pumps, intracellular and extracellular accumulation in parallel in order to maintain metal levels below a toxic threshold and overcome the effects of high concentrations. These findings are an approach of an energy-dependent efflux system to eliminate excessive amounts of crude oil-associated metals in Bacillus. B. cereus M6 may potentially be useful in designing improved strategies for the bioremediation of soils polluted with metals. Additionally, the prediction model developed would be useful for improving the monitoring of in vitro and in vivo bioremediation processes.

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“…Bioaccumulation by growing cells is a potential method for the removal of heavy metal from the environment [12,13].…”

Section: Introductionmentioning

confidence: 99%

Cobalt(II) bioaccumulation and distribution in Rhodopseudomonas palustris

Gao

Wang

Zhang

et al. 2017

Biotechnology & Biotechnological Equipment

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Bioaccumulation by growing cells is a potential technique for the removal of heavy metals from wastewater. Cobalt bioaccumulation by Rhodopseudomonas palustris via different metabolic pathways was investigated for various pH levels, temperatures, co-cations and initial Co 2+ concentrations. The distribution of cobalt and its chemical nature in R. palustris were examined by cell compartmentalization and X-ray diffraction analysis. Our results indicated that biomass and bioaccumulation capacity were superior under aerobic conditions in the dark at pH 6.5 and 30 C. Cobalt removal efficiency and bioaccumulation capacity were, respectively, 84. 9% and 287.18 . Cobalt was distributed between the cell surface, periplasmic space, membrane and cytoplasm. The periplasmic space and membrane accounted for 57.37% and 27.95% of Co 2+ uptake, respectively. X-ray diffraction analysis showed that cobalt ions were predominantly deposited in the cell as cobalt phosphate octahydrate. These results demonstrate that R. palustris could be used to remove cobalt from industrial wastewater; it would therefore be beneficial to further study the mechanism of cobalt bioaccumulation in this organism.

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Bioaccumulation characterization of cadmium by growing Bacillus cereus RC-1 and its mechanism

Cited by 125 publications

References 47 publications

Cadmium resistance mechanism in Escherichia coli P4 and its potential use to bioremediate environmental cadmium

Cadmium resistance mechanism in Escherichia coli P4 and its potential use to bioremediate environmental cadmium

Mathematical Modelling of Toxic Metal Uptake and Efflux Pump in Metal-Resistant Bacterium Bacillus cereus Isolated From Heavy Crude Oil

Cobalt(II) bioaccumulation and distribution in Rhodopseudomonas palustris

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