Bioaccumulation of Cadmium from Durum Wheat Diets in the Livers and Kidneys of Mice

Chan, D. Y.; Black, W. D.; Hale, Beverley

doi:10.1007/s001280000035

Cited by 10 publications

(11 citation statements)

References 8 publications

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“…The difference in bioaccessibility between the diets (although not the absolute bioavailability in vivo) would be predicted by the dialysis assay, but not the Caco-2 cell or PBET assay. The grain/mouse study [21] did not identify a difference in bioavailability between Cd-incorporated and Cd-amended grain, although for the liver, although the bioaccumulation index (mass of Cd in liver plus kidney divided by mass of Cd in experimental diet) for Cd-amended grain was 0.0061 versus 0.0028 for Cd-incorporated grain. For the grain, the Caco-2 cell assay suggested that Cd from the amended grain was more bioaccessible that the incorporated Cd ( Table 3).…”

Section: In Vivo Studiesmentioning

confidence: 80%

“…Accumulation of Cd in liver plus in kidney of mice fed grain in which Cd had either been incorporated during growth, or amended prior to feeding vs. total dietary dose of Cd from grain. Data are from Chan et al [21] match. The in vivo studies cited here did not measure total body burdens of Cd, but did quantify accumulation in the liver and kidney, the 2 organs most likely to accumulate Cd in mammals.…”

Section: In Vivo Studiesmentioning

confidence: 98%

“…For both in vivo studies [20,21] as well as the present in vitro studies, lettuce (Latuca sativa L. cv. Ostinata) and durum wheat (Triticum turgidum L. var durum) were grown hydroponically, with and without Cd(NO 3 ) 2 ·H 2 O (500 μg Cd/L) to generate the "incorporated" and "control" diet material.…”

Section: Diet Materialsmentioning

confidence: 99%

“…Ostinata) and durum wheat (Triticum turgidum L. var durum) were grown hydroponically, with and without Cd(NO 3 ) 2 ·H 2 O (500 μg Cd/L) to generate the "incorporated" and "control" diet material. The protocol is fully described in Chan et al; [21] briefly, seeds are germinated individually in rockwool plugs, which after 14 days of growth are placed into a recirculating, aerated hydroponic culture system. Then, 80 L of half-strength Hoaglands solution, [22] modified by the addition of EDTA for the chelation of Fe, supplied each group of 14 plants; the volume of the solution was restored twice per week, and completely replaced every 2 weeks.…”

Section: Diet Materialsmentioning

confidence: 99%

“…The mice were similarly fed durum grain that had either been amended with, or grown supplied with, Cd·(NO 3 ) 2 ·H 2 O, so that the Cd was differentially associated with the grain. [21] Each diet was administered at 4 doses; all the animals were sacrificed after 10 d for determining Cd accumulation in the liver and kidney samples.…”

Section: In Vivo Studiesmentioning

confidence: 99%

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Cadmium bioavailability and bioaccessibility as determined by in vitro digestion, dialysis and intestinal epithelial monolayers, and compared to in vivo data

Chan¹,

Black²,

Hale³

2007

Journal of Environmental Science and Health, Part A

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The objective of the study was to compare in vivo estimates of Cd bioavailability in two diet materials (lettuce and durum wheat grain) with bioaccessibility estimates from three in vitro methods. For both dietary materials, the Cd was either incorporated during growth or applied topically as a soluble salt just prior to experimentation. Simulated gastric/intestinal digestion using a physiologically based extraction technique (PBET) solubilized less than 56% (lettuce) or 13% (grain) of the Cd that was either incorporated into the plant tissues during growth, or added to the plant tissues before experimentation, as Cd(NO3)2.H2O. Amended diets could not be distinguished from incorporated diets. More of the Cd solubilized from amended lettuce than from incorporated lettuce moved to the outside of MWCO 10 kD and 25 kD dialysis sacs; no difference between the amended and incorporated diets was observed for grain. The percentage of lettuce-Cd solubilized by the PBET and sorbed by Caco-2 cells was greater for incorporated than for amended lettuce; for Cd in grain, the reverse occurred. As expected, none of the in vitro estimates of bioaccessibility were the same percentage of Cd in the lettuce or grain as was measured as bioavailable in vivo. The in vitro assays all predicted that substantially less than 100% of the Cd in the foods would be bioavailable, as was identified in vivo, and simulating intestinal selectivity improved the comparison to in vivo. Some of the in vitro assays identified subtle differences between the diets (i.e., amended vs. incorporated) that were consistent with in vivo studies, and with speculated differences in Cd speciation; this suggests their potential usefulness for the study of modifiers to dietary Cd bioavailability.

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Section: In Vivo Studiesmentioning

confidence: 80%

Section: In Vivo Studiesmentioning

confidence: 98%

Section: Diet Materialsmentioning

confidence: 99%

Section: Diet Materialsmentioning

confidence: 99%

Section: In Vivo Studiesmentioning

confidence: 99%

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