Bioaccumulation of copper and toxic effects on feeding, growth, fecundity and development of pond snail Lymnaea luteola L.

Das, Sangita; Khangarot, B. S.

doi:10.1016/j.jhazmat.2010.09.033

Cited by 74 publications

(44 citation statements)

References 37 publications

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“…Concentrations that affect locomotory behavior are often similar to those causing growth impairment in the longer-term (Little and Finger, 1990;Melvin and Wilson, 2013). Detrimental effects of chemicals on locomotory behavior have also been reported for various invertebrate species (Salanki, 2000;Tu et al, 2010;Hellou, 2011;Pekar, 2012;Oliveira et al, 2013;Pereira et al, 2013;Fong and Ford, 2014) and in some studies directly shown to coincide with reduction in food intake as well (Das and Khangarot, 2011;Agatz et al, 2012;Nyman et al, 2013). This suggests that the role in growth impairment of both KEs, impairment of locomotion and reduction in food intake, may be conserved in a broad spectra of taxa.…”

Section: Ke ''Reduction In Food Intake'': Choice Justification and Exmentioning

confidence: 86%

Development and application of the adverse outcome pathway framework for understanding and predicting chronic toxicity: II. A focus on growth impairment in fish

et al. 2015

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Adverse outcome pathways (AOPs) organize knowledge on the progression of toxicity through levels of biological organization. By determining the linkages between toxicity events at different levels, AOPs lay the foundation for mechanism-based alternative testing approaches to hazard assessment. Here, we focus on growth impairment in fish to illustrate the initial stages in the process of AOP development for chronic toxicity outcomes. Growth is an apical endpoint commonly assessed in chronic toxicity tests for which a replacement is desirable. Based on several criteria, we identified reduction in food intake to be a suitable key event for initiation of middle-out AOP development. To start exploring the upstream and downstream links of this key event, we developed three AOP case studies, for pyrethroids, selective serotonin reuptake inhibitors (SSRIs) and cadmium. Our analysis showed that the effect of pyrethroids and SSRIs on food intake is strongly linked to growth impairment, while cadmium causes a reduction in growth due to increased metabolic demands rather than changes in food intake. Locomotion impairment by pyrethroids is strongly linked to their effects on food intake and growth, while for SSRIs their direct influence on appetite may play a more important role. We further discuss which alternative tests could be used to inform on the predictive key events identified in the case studies. In conclusion, our work demonstrates how the AOP concept can be used in practice to assess critically the knowledge available for specific chronic toxicity cases and to identify existing knowledge gaps and potential alternative tests.

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Section: Ke ''Reduction In Food Intake'': Choice Justification and Exmentioning

confidence: 86%

Development and application of the adverse outcome pathway framework for understanding and predicting chronic toxicity: II. A focus on growth impairment in fish

et al. 2015

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“…Therefore the identifiable snail neurons provide a suitable tool to characterize the interactions between the ACh receptors (AChRs) and potentially toxic substances including heavy metals, insecticides or mycotoxins (Arvanov et al, 1993;Gyori et al, 1994;Gyori et al, 2007). The roles of many identified neurons in controlling the relatively simple behavioural patterns of the animals have also been established (Chase, 2002), meaning that toxin-evoked functional changes of the nervous system will refer to known behavioural alterations of the intact animal (Dobranskyte et al, 2004;Vehovszky et al, 2007;Das and Khangarot , 2011).…”

Section: Introductionmentioning

confidence: 99%

Neonicotinoid insecticides inhibit cholinergic neurotransmission in a molluscan (Lymnaea stagnalis) nervous system

et al. 2015

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Neonicotinoids are highly potent and selective systemic insecticides, but their widespread use also has a growing impact on non-target animals and contaminates the environment, including surface waters. We tested the neonicotinoid insecticides commercially available in Hungary (acetamiprid, Mospilan; imidacloprid, Kohinor; thiamethoxam, Actara; clothianidin, Apacs; thiacloprid, Calypso) on cholinergic synapses that exist between the VD4 and RPeD1 neurons in the central nervous system of the pond snail Lymnaea stagnalis. In the concentration range used (0.01-1 mg/ml), neither chemical acted as an acetylcholine (ACh) agonist; instead, both displayed antagonist activity, inhibiting the cholinergic excitatory components of the VD4-RPeD1 connection. Thiacloprid (0.01 mg/ml) blocked almost 90 % of excitatory postsynaptic potentials (EPSPs), while the less effective thiamethoxam (0.1mg/ml) reduced the synaptic responses by about 15 %. The ACh-evoked membrane responses of the RPeD1 neuron were similarly inhibited by the neonicotinoids, confirming that the same ACh receptor (AChR) target was involved. We conclude that neonicotinoids act on nicotinergic acetylcholine receptors (nAChRs) in the snail CNS. This has been established previously in the insect CNS; however, our data indicate differences in the background mechanism or the nAChR binding site in the snail. Here we provide the first results concerning neonicotinoid-related toxic effects on the neuronal connections in the molluscan nervous system. Aquatic animals, including molluscs, are at direct risk while facing contaminated surface waters, and snails may provide a suitable model for further studies of the behavioural/neuronal consequences of intoxication by neonicotinoids.Tihany, Hungary, July 22, 2015 Dr. Mikko Nikinmaa Editorial Office Aquatic ToxicologyDear Dr Nikinmaa, Thank you very much for your response to our manuscript submission. We gratefully thank you and our reviewers for reading the manuscript, and we appreciate all the helpful comments made.After carefully checking the Reviewers' comments we improved the manuscript on the basis of their suggestions. Please find our detailed answers to the Reviewers below. Reviewer #1:We greatly appreciate the Reviewer's comment "one of the best written manuscripts that I have reviewed" Minor points Materials and Methods How was Lymnaea saline obtained? Is this the modified HiDi saline? If not, what is HiDi?For isolating the monosynaptic components of the synaptic responses between two neurons the perfusion chamber was filled with a modified saline which reduces the polysynaptically evoked postsynaptic responses and enhances the monosynaptic component (Berry and Pentreath, 1976). This modified saline contains higher amount of divalent cations (Ca 2+ and Mg 2+ ), therefore often nicknamed as HiDi saline (Brierly et al., 1997, Sivaramakrishnan et al., 2013. We added details to the text accordingly Electrophysiological recordingThere is a Winlow 1991 anda Syed et al. 1990 (PNEC, EC50, mechanistic...) We...

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“…It has been used for bioaccumulation and toxicity studies using the digestive gland (DG) [9,10], and the effects on the reproductive system have been studied in the albumen gland (AG) [11]. Moreover, this invertebrate species is a unique model in neurobiology [12] since the central nervous system (CNS) is easily accessible as well as relatively simple to dissect.…”

Section: Introductionmentioning

confidence: 99%

Cross-platform metabolic profiling: application to the aquatic model organism Lymnaea stagnalis

et al. 2015

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The freshwater pond snail Lymnaea stagnalis is used in several studies on molecular and behavioral neurobiology and ecotoxicology showing its successful application as a model organism. In the present study, a cross-platform metabolomic approach has been evaluated to characterize the organ molecular phenotypes of L. stagnalis central nervous system (CNS), digestive gland (DG), and albumen gland (AG). Two types of tissue disruption methods were evaluated of which beads beating was the preferred method. To obtain a broad picture of the hydrophilic and lipophilic metabolome, two complementary analytical platforms have been employed: liquid chromatography (LC) and gas chromatography (GC) coupled to high-resolution mass spectrometry. Furthermore, to increase the power to separate small polar metabolites, hydrophilic interaction liquid chromatography (HILIC) was applied. The analytical platform performances have been evaluated based on the metabolome coverage, number of molecular features, reproducibility, and multivariate data analysis (MVDA) clustering. This multiplatform approach is a starting point for future global metabolic profiling applications on L. stagnalis.

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Bioaccumulation of copper and toxic effects on feeding, growth, fecundity and development of pond snail Lymnaea luteola L.

Cited by 74 publications

References 37 publications

Development and application of the adverse outcome pathway framework for understanding and predicting chronic toxicity: II. A focus on growth impairment in fish

Development and application of the adverse outcome pathway framework for understanding and predicting chronic toxicity: II. A focus on growth impairment in fish

Neonicotinoid insecticides inhibit cholinergic neurotransmission in a molluscan (Lymnaea stagnalis) nervous system

Cross-platform metabolic profiling: application to the aquatic model organism Lymnaea stagnalis

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