Bioaccumulation of Heavy Metals and Physiological/Histological Changes in Gonads of Catfish (Clarias gariepinus) inhabiting Lake Maryout, Alexandria, Egypt

Abdel-Kader, Heba H.; Mourad, Mohamed

doi:10.21608/ejabf.2019.32036

Cited by 9 publications

(6 citation statements)

References 27 publications

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“…In the studied organs, level of T-Hg was significantly elevated in the ovary and testis from upstream sites. Several factors such as organ-specific biological activity and metabolism actively contribute to changes in the rates of incorporation of toxic metals into the body's various tissues (Gorski et al, 2003). Also, interactions between the physical and chemical characteristics of environment influence the retention of chemical pollutants by biological organisms (Kainz et al, 2003;Mille et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Assessment of the Reproductive Health Status of Clarias Gariepinus (Burchell, 1822) in a River Impacted by Mercury from Clandestine Gold Mining: A Case Study of Bagoue River, Côte d’Ivoire

guessan

Pèlèbè²,

Boussou³

et al. 2020

AST

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Pollution of aquatic ecosystems often results in adverse environmental disturbances, including individual and/or sub-individual changes in fish. The aim of the present study was to examine possible reproductive health effects in male and female, based on a histological assessment of Clarias gariepinus sampled in Bagoue River impacted by mercury from the artisanal gold mining. Fish were collected between August 2018 and April 2019 during two seasons from four stations differently impacted. A histological assessment was done on three target fish organs (liver, ovary and testis). Water and sediment samples were analysed for total mercury (T-Hg) concentration. Histological alterations were semi-quantitatively assessed, and the results were classified according to the severity of the histological response identified. Microscopic assessment revealed alterations of the gonads and liver tissues. A higher incidence of reaction pattern (Regressive changes, Inflammation and Circulatory disturbance) was found in liver tissues than in gonads tissues (Regressive changes). T-Hg levels were higher upstream than downstream in ovaries and testis tissues. Paradoxically, the lesions observed in these organs were more pronounced downstream than those found in fish from upstream during rainy season. According to total index, the organs examined downstream suffered severe damage unlike upstream sites. Also, comparison of degree of alteration between testes and ovaries indicates a greater impact of ovarian structures especially on downstream sites. The effect of anthropogenic impact appears to be visible downstream as the concentration of chemical pollutants and exposure time determine the severity of the damage. Alterations in ovarian follicles, spermatogenic cells and liver tissues may reflect damage to the process of gametogenesis, which may reduce the reproductive capacity of individuals over the long term.

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Section: Discussionmentioning

confidence: 99%

Assessment of the Reproductive Health Status of Clarias Gariepinus (Burchell, 1822) in a River Impacted by Mercury from Clandestine Gold Mining: A Case Study of Bagoue River, Côte d’Ivoire

guessan

Pèlèbè²,

Boussou³

et al. 2020

AST

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“…Depletion of the lipid and protein content of Pb-exposed Catla catla muscles (Palaniappan et al 2008), can be caused by tissue organization and its use in lipoprotein cell repair, essential cellular elements of cytoplasmic cell membranes and organelles (Filipovic and Raspor 2003). Abdel-Kader and Mourad (2019b) showed an increase in protein content from 56 to 27 mg/g and 26 to 32 mg/g in lipid content from two basins at Maryout Lake. Abdel-Kader and Mourad (2020) showed that the protein 57.16± 2.6 mg/g and lipid content 11.98±1.1 mg/g of T. Zillii were greater than of C. gariepinus 56.16±3.0 mg/g and 11.65±0.7 mg/g, respectively, from Burullus Lake, Egypt…”

Section: Proximate Body Compositionmentioning

confidence: 99%

“…The biochemical pro le in sh is a sensitive index of sh metabolism assessment under metallic stress. Several studies demonstrated that sh exposed to toxic elements such as Cd, Pb, Hg, As and Al recorded low levels of proteins and lipids (Sobha et al 2007;Selvam et al (2014); Ayanda et al 2018;Abdel-Kader and Mourad 2019b;Abdel-Kader and Mourad 2020).…”

Section: Introductionmentioning

confidence: 99%

“…The antioxidant defense systems of sh tissues, particularly the liver, protect them against oxidative stress (Basha and Rani, 2003;Atli and Canli, 2007). Several researchers estimated the activity of the antioxidant enzymes after exposure to toxic elements such as Cd, Pb, Hg, Al, and As in the different sh species collected from different freshwater lakes (Barata et al 2005;Monteiro et al 2010;Abdelazim et al 2018;Elarabany and Bahnasawy 2019;Abdel-Kader and Mourad 2019b;Abdel-Kader and Mourad 2020), they recorded a reduction in the activity of the antioxidant enzymes after Cd, Pb, Hg, As and Al exposure.…”

Section: Introductionmentioning

confidence: 99%

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Is the consumption of Tilapia zillii and Clarias gariepinus from Edku Lake — El Beheira Governorate —Egypt safe for humans: a physiological and biochemical study and determination of five trace elements

Abdel-Kader

Mourad

Haredi

2021

Preprint

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The Egyptian government seeks to develop the northern lakes, including Edku Lake in the El Beheira governorate, to enhance the physiological functions of fish, and thus, improve their quality and promote their products. Therefore, this study was performed on the two cheapest fish species T. zillii and C. gariepinus in the Egyptian market, by studying proximate body composition, antioxidant enzyme activity, and bioaccumulation of some trace elements. In this study, T. zillii showed the highest mean of carbohydrates (16.11± 0.67 mg/g), fats (24.21 ± 0.93mg/g), ash (7.33 ±1.00 %), and water content (78.33 ± 1.85 %) whereas, the highest mean of protein content was recorded in C. gariepinus (64.33 ±1.01 mg/g). Antioxidant activity GSH (41.66 ± 2.01mg/g), CAT (21.66 ± 2.6 U/g), GPx (36.5 ± 3.01 mU/L), and GR (41.16 ± 1.0 U/L) in the liver of T. zillii demonstrated insignificant decrease, except GSH and CAT was significant at (p < 0.05). SOD activity showed an insignificant decrease in T. zillii (47±3.5 U/g). Concerning trace element concentrations, C. gariepinus demonstrated an increase in five trace elements than T. zillii. The abundance of the elements is in the order as Hg< Al < Cd <Pb < As in T. zillii and C. gariepinus muscles. The highest As was recorded in C. gariepinus (1.54 ± 0.08 μg/g), whereas, the lowest Hg was recorded in T.zillii (0.47 ± 0.08 μg/g). In this research, the concentrations of the trace element in the two studied fish species were above the limits permitted by EOSQC, FAO/WHO, and the EU'. The estimated weekly intake (EWI) of the elements concentrated in the two fish species was unsafe for young people (40 Kg), and children (15 Kg), whereas it was safe for consumption by adults (70 kg) with advice on safe levels of consumption for C. gariepinus for adults. So, this study recommended that the children should consume less than 16.12857 g/day or 112.90 g/week T.zillii muscle and 13.27 g/day or 92.92 g/week C. gariepinus muscle. Moreover, Youth should consume less than 43.01 g/day or 301.7g/ week T.zillii muscle and 35.39714 g/day or 247.78 g/week C. gariepinus muscle. Finally, adult should consume less than 23.1 g/day and 161.7 g/week T.zillii muscle, and 61.94286 g/day or 433.6 g/week C. gariepinus muscle to maintain their safety.

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“…The fish bioassay helps measure heavy metals contamination via the metal bio-magnification process ( 21 ). In freshwater, there are many physiological and oxidative stress biomarkers that depend on antioxidant enzymes, i.e., superoxide dismutase (SOD), catalase (CAT), and glutathione reductase (GR), as well as non-enzymatic antioxidants such as reduced glutathione (GSH) ( 22 – 24 ). The micronucleus (MN) frequency test is a robust technique among all predominant biomarkers to determine the cytogenetic damage caused by environmental toxicity and estimate water quality ( 3 , 25 ).…”

Section: Introductionmentioning

confidence: 99%

Genotoxicity and Trace Elements Contents Analysis in Nile Tilapia (Oreochromis niloticus) Indicated the Levels of Aquatic Contamination at Three Egyptian Areas

et al. 2022

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The toxic waste and pollutants of heavy metals continuously pollute freshwater aquatic reservoirs, which have severe implications on aquatic life and human health. The present work aims to evaluate trace elements (Zn, Mn, Cu, Cd, and Pb) along with three sites, Mariout Lake, Abbassa, and River Nile Aswan in Egypt, using Nile tilapia (Oreochromis niloticus) as bioindicator. The quality assurance, health-risk assessment, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), quantitative polymerase chain reaction (qPCR), and micronucleus test were performed to investigate the effect of different trace elements on Hsp70 gene level and micronuclei formation. We observed the highest expression of Hsp70 protein band of 70 KD and stress-responsive Hsp70 gene in the liver followed by gills of Nile tilapia caught from Mariout and Abbassa, but the lowest expression was in Nile tilapia caught from Aswan. Obvious micronuclei were observed under the microscope in erythrocytes, and their number was gradually decreased in the following manner: Mariout > Abbassa > Aswan. Noticeably, Cu, Zn, and Mn contents were low. Still, Pb and Cd contents were higher than the toxicity level recommended by the Food and Agriculture Organization (FAO), The World Health Organization (WHO), and the European Commission (EC). These results showed that Hsp70's appearance at the two levels of mRNA and protein is an effective indicator for aquatic pollution besides the aberration at the chromosome level represented in the micronucleus test. Furthermore, these results showed that Nile tilapia of the Aswan region had comparatively low trace elements contamination and were suitable for consumption.

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Bioaccumulation of Heavy Metals and Physiological/Histological Changes in Gonads of Catfish (Clarias gariepinus) inhabiting Lake Maryout, Alexandria, Egypt

Cited by 9 publications

References 27 publications

Assessment of the Reproductive Health Status of Clarias Gariepinus (Burchell, 1822) in a River Impacted by Mercury from Clandestine Gold Mining: A Case Study of Bagoue River, Côte d’Ivoire

Assessment of the Reproductive Health Status of Clarias Gariepinus (Burchell, 1822) in a River Impacted by Mercury from Clandestine Gold Mining: A Case Study of Bagoue River, Côte d’Ivoire

Is the consumption of Tilapia zillii and Clarias gariepinus from Edku Lake — El Beheira Governorate —Egypt safe for humans: a physiological and biochemical study and determination of five trace elements

Genotoxicity and Trace Elements Contents Analysis in Nile Tilapia (Oreochromis niloticus) Indicated the Levels of Aquatic Contamination at Three Egyptian Areas

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