Bioaccumulation of pesticides and genotoxicity in anurans from southern Brazil

Leaf-Litter Anurans in An Agricultural Landscape in the araucaria Forest: A Case Study for Southern Brazil

“…Although we did not evaluate agrochemical usage in the plantation, it should be noted that these substances could represent a threat to the anurans in the agricultural landscape, given that these substances can promote cellular and genetic alterations leading to morphological abnormalities, thereby affecting population viability (Laurentino et al, 2016; Lenhardt et al, 2017; Assis et al, 2021). Many studies have described the negative effects of agrochemicals on anuran populations (Guerra and Aráoz, 2016; Ascoli-Morrete et al, 2019; Agostini et al, 2020; Ascoli-Morrete et al, 2022; Sánchez-Domene et al, 2022). In this sense, the occurrence of endangered species in plantations, such as Melanophryniscus devincenzii, which occurred almost exclusively in this environment, is concerning because the viability and maintenance of their populations can be threatened.…”

Development and application of a mini-QuEChERS method for the determination of pesticide residues in anuran adipose tissues

“…[7][8][9][10] Pesticides can have many adverse effects on amphibians, including decreased growth and developmental rates, increased incidence of external abnormalities, impaired reproductive potential, and, most drastically, death. [11][12][13][14][15][16][17] A part of the anuran lifetime occurs in ponds, rivers, streams, and temporary pools associated with agricultural areas with intense pesticide usage. 15 Some anuran species that inhabit these environments may be exposed to the toxic effects of pesticide residues.…”

“…Pesticide residues in non-endangered anuran tissue can be a marker in conrming the connection between the disappearance of amphibians and habitat contamination with pesticides. 15,17 The detection and quantication of pesticide residues in anuran tissues require an invasive step of removing part of the tissue, which usually occurs post-mortem of the animal. 18,19 For this reason, there are relatively few studies involving the detection of pesticide residues directly in tissues.…”

“…7–10 Pesticides can have many adverse effects on amphibians, including decreased growth and developmental rates, increased incidence of external abnormalities, impaired reproductive potential, and, most drastically, death. 11–17…”

“…Pesticide residues in non-endangered anuran tissue can be a marker in confirming the connection between the disappearance of amphibians and habitat contamination with pesticides. 15,17…”