Bioactivation of zirconia surface with laminin protein coating via plasma etching and chemical modification

3D-Printed Poly(ε-Caprolactone)/Hydroxyapatite Scaffolds Modified with Alkaline Hydrolysis Enhance Osteogenesis In Vitro

“…The reduced strut dimension caused an increase in the pore size. In order to quantitatively analyze the existence of HA particles on the surface of the scaffolds [32], Alizarin Red S staining, which can detect a calcified matrix, was performed (Figure 2a). The scaffolds stained with Alizarin Red S were de-stained with a 10% cetylpyridinium chloride/10 mM sodium phosphate solution and incubated at room temperature for 30 min.…”

Laminin 332-functionalized coating to regulate the behavior of keratinocytes and gingival mesenchymal stem cells to enhance implant soft tissue sealing

“…The sizes of the globular particles were around 2–3 μm, suggesting the formation of oligomers since the size of an extended LN332 molecule is 120 nm at most [ 22 ]. Tapia-Lopez et al have used LN332 protein to modify zirconia and found that the sizes of LN332 protein are around 200–400 nm with a little squashed shape [ 12 ]. The variance in the shape and size of LN332 after loading onto biomaterials may be associated with the different biomaterial substrates, protein concentration and binding mechanism.…”

“…Compared with TiP, TiPLN possesses a reduced contact angle of 53.42°, which is still larger than that of Ti. LN332 has both hydrophobic and hydrophilic sites [ 12 ]. After the binding of hydrophobic sites with PDLLA, the hydrophilic sites are accordingly exposed, leading to enhanced hydrophilicity.…”

“…[ 9 ] have constructed LN332 coating by immersing Ti alloy into LN332 protein solution and found that the adhesion ability of gingival epithelial cells was enhanced. Tapia-Lopez et al [ 12 ] have etched and activated the zirconia surface with argon plasma, and then LN332 was bonded on the zirconia surface by adsorption, which significantly improved epithelial cell spreading and adhesion. Our results are consistent with theirs, collectively confirming the enhancing effect of LN332 on epithelial cell/keratinocyte spreading and adhesion.…”

RGD Peptide-Functionalized Polyether Ether Ketone Surface Improves Biocompatibility and Cell Response

“…The samples were rinsed with PBS, and the cytoskeleton was stained according to a protocol described previously. 26 Briefly, cells were washed with PBS and fixed with 3.75% paraformaldehyde for 15 min at 4 °C. Subsequently, a permeabilization process was carried out with triton X-100 at 0.2% for 10 min at room temperature followed by blocking with lacteous protein (10% in PBS; 1 h) at room temperature.…”