Bioactivation Treatment with Mixed Acid and Heat on Titanium Implants Fabricated by Selective Laser Melting Enhances Preosteoblast Cell Differentiation

Le, Phuc Thi Minh; Shintani, Seine A.; Takadama, Hiroaki; Ito, Morihiro; Kakutani, Tatsuya; Kitagaki, Hisashi; Terauchi, Shuntaro; Ueno, Takaaki; Nakano, Hirofumi; Nakajima, Yoshiyuki; Inoue, Kohei; Matsushita, Tomiharu; Yamaguchi, Seiji

doi:10.3390/nano11040987

Cited by 10 publications

(6 citation statements)

References 56 publications

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“…Furthermore, the relative gene expression of ALP and COL were up-regulated for TICT samples associated with calcium titanate produced after 48 h in SBF [65]. Similar results were found for MC3T3-E1 cells seeded on a selective laser melting -Ti surface, which showed a strong tendency towards osteogenic differentiation by significantly increased expression of alkaline phosphatase, osteocalcin, Runx2 and osteopontin [66].…”

Section: Discussionsupporting

confidence: 74%

Comparison of hydrophilic and hydrophobic nano topographic surfaces of titanium alloys on pre-osteoblastic cell interaction

Oliveira¹,

Micocci²,

Almeida

et al. 2023

Biomed. Phys. Eng. Express

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This work aimed to assess the influence of different structured substrates with hydrophilic and hydrophobic properties on micro and nano topographies developed on titanium alloys over pre-osteoblastic cell behavior. Nano topography influences small dimension levels of cell morphology by inducing filopodia formation in cell membranes, irrespectively to the wettability behavior of the surface. Therefore, micro and nanostructured surfaces of titanium-based samples using different techniques of surface modification processing, such as chemical treatments, micro-arc anodic oxidation (MAO), and MAO combined to laser irradiation were developed. Isotropic and anisotropic texture morphologies, wettability, topological parameters and compositional alterations were measured after the surface treatments. Finally, cell viability, adhesion and morphological responses were assessed to investigate the influence of distinct topologies on osteoblastic cells aiming to encounter the conditions to better promote mineralization events. Our study demonstrated that the hydrophilic behavior improves cell adhesion, amplified when effective surface area increases. Surfaces presenting nano topography have a direct influence on cell morphology and play a key role for filopodia formation.

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Section: Discussionsupporting

confidence: 74%

Comparison of hydrophilic and hydrophobic nano topographic surfaces of titanium alloys on pre-osteoblastic cell interaction

Oliveira¹,

Micocci²,

Almeida

et al. 2023

Biomed. Phys. Eng. Express

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“…These micro/nanoscale structures observed through SEM on the AHT-Sr implant surface may enhance bone formation. In addition, osteoblast attachment could be promoted by implants with higher surface hydrophilicity ( Le et al, 2021 ). Kubo et al (2009) have reported that uniformly distributed 300-nm nano surface structures significantly enhance ALP activity, mRNA expression of Col1, and Ocn, and total calcium deposition in BMSCs.…”

Section: Discussionmentioning

confidence: 99%

Strontium-doping promotes bone bonding of titanium implants in osteoporotic microenvironment

Geng

Wang

Lin

et al. 2022

Front. Bioeng. Biotechnol.

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Osteoporosis is a major challenge to oral implants, and this study focused on improving the osseointegration ability of titanium (Ti) implants in osteoporosis environment via surface modification, including doping of strontium ion and preparation of nanoscale surface feature. Our previous studies have shown that strontium (Sr) ions can enhance osteogenic activity. Therefore, we aimed to comprehensively evaluate the effect of hydrothermal treatment of Sr-doped titanium implant coating on bone-binding properties in the microenvironment of osteoporosis in this study. We fabricated Sr-doped nanocoating (AHT-Sr) onto the surface of titanium implants via hydrothermal reaction. The rough Sr-doping had good biological functions and could apparently promote osteogenic differentiation of osteoporotic bone marrow mesenchymal stem cells (OVX-BMSCs). Most importantly, AHT-Sr significantly promoted bone integration in the osteoporosis environment. This study provides an effective approach to implant surface modification for better osseointegration in an osteoporotic environment.

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“…The spheroidal‐like bone apatite was only generated on the surface of SLM Ti using acid‐heat treatment. [ 145 ] In addition, the microhardness of SLM implants was increased by acid‐heat treatment due to the formation of oxide coating. [ 146 ] Table 1 summarizes the acid treatment studies of SLM Ti and its alloys.…”

Section: Chemical Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

Surface Modification of Porous Titanium and Titanium Alloy Implants Manufactured by Selective Laser Melting: A Review

Liu,

Li,

Wang

et al. 2023

Adv Eng Mater

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Titanium (Ti) and its alloy implants with porous structure manufactured by selective laser melting (SLM) can match elastic modulus of human bone to reduce the stress‐shielding effect and satisfy the personalized requirement in orthopedic surgery. Compared with conventional casting and forging Ti and its alloy implants, SLM implants possess unique microstructural features and excellent comprehensive mechanical properties. However, the un‐melted powder particles inevitably adhere to the surfaces of SLM implants, which may result in excessive surface roughness and potential health risks. Moreover, there are significant issues encountered, such as bioactivity, toxicity, antibacterial activity, corrosion and wear resistance. Consequently, surface modification methods are essential to remove the un‐melted powder particles and improve biological and mechanical properties of SLM implants. Herein, the research efforts focus exclusively on chemical (acid treatment, alkali treatment, sol‐gel, chemical vapor deposition and atomic layer deposition) and electrochemical methods (anodization and microarc oxidation) for SLM Ti and its alloy implants, especially for porous structures. Particularly, the characteristics of these methods are summarized, and their commonly used pre‐ and post‐treatment methods are introduced. In addition, the development trends and challenges in surface modification of SLM Ti and its alloy implants are discussed.This article is protected by copyright. All rights reserved.

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Bioactivation Treatment with Mixed Acid and Heat on Titanium Implants Fabricated by Selective Laser Melting Enhances Preosteoblast Cell Differentiation

Cited by 10 publications

References 56 publications

Comparison of hydrophilic and hydrophobic nano topographic surfaces of titanium alloys on pre-osteoblastic cell interaction

Comparison of hydrophilic and hydrophobic nano topographic surfaces of titanium alloys on pre-osteoblastic cell interaction

Strontium-doping promotes bone bonding of titanium implants in osteoporotic microenvironment

Surface Modification of Porous Titanium and Titanium Alloy Implants Manufactured by Selective Laser Melting: A Review

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