Bioactive Components of Helicteres hirsuta Collected in Thua Thien Hue

Abstract

Objectives: In this study, we investigate the bioactive potential of Helicteres hirsuta Lour. (H. hirsuta L.). Methods: H. hirsuta collected in Thua Thien Hue province was used. The methanolic extract of roots and leaves was studied for its biochemical components based on the High-Performance Liquid Chromatography method and also tested for antioxidant activities Mention how the test was carried out and the endpoints considered. Findings : The results show that some biochemical components contained in the dry materials of H. hirsuta leaves and roots are safe and suitable, allowing use in the extraction and collection of total flavonoids. The optimal extraction conditions using the ultrasonic bath method included a time of 50 min, a material/solvent ratio of 1:20 (g/mL), ultrasonic frequency (12 Hz), and a solvent concentration of methanol of 50% (v/v). In this condition, the highest total flavonoid content obtained from H. hirsuta leaves and roots were 0.841 mg CE/mL extract (leaf) and 0.416 mg CE/mL extract (root), respectively. The DPPH free radical scavenging ability at 50% concentration (IC 50 ) of leaf and root extracts varied with IC 50 of 0.778 mL and 0.433 mL, respectively. The methanol extract from the leaf and root of H. hirsuta has the best inhibitory effect on the human liver cancer cell line Hep-G2 (IC 50 leaf = 177.22 µg/mL, and IC 50 root = 165.21 µg/mL). Novelty: The results of this study are valuable reference information for exploiting, using, and processing products from H. hirsuta trees collected in Thua Thien Hue in the future.