Bioactive Constituents from Chinese Natural Medicines. XXXIII. Inhibitors from the Seeds of Psoralea corylifolia on Production of Nitric Oxide in Lipopolysaccharide-Activated Macrophages

Abstract

The methanolic extract from the seeds of Psoralea corylifolia was found to inhibit production of nitric oxide (NO) in lipopolysaccharide-activated mouse peritoneal macrophages. Among the isolated compounds, bavachinin (IC 50 ‫62؍‬ m mM), isobavachalcone (17 m mM), neobavaisoflavone (ca. 29 m mM), corylifol A (ca. 21 m mM), and psoralidin (ca. 23 m mM) significantly inhibited the accumulation of nitrite (NO 2 ؊ ) as a marker of production of NO. Bakuchiol, which is mainly contained in the extract, also showed weak activity at 10 m mM, but cytotoxic effects were observed more than 30 m mM.

Isobavachalcone attenuates lipopolysaccharide-induced ICAM-1 expression in brain endothelial cells through blockade of toll-like receptor 4 signaling pathways

“…Isobavachalcone is reported to inhibit NO production and iNOS expression in LPS-activated macrophages (Matsuda et al, 2009;Shin et al, 2013), but neither the underlying mechanisms involved nor the effects on cerebral inflammation are well understood. In our study, we discovered that isobavachalcone inhibited leukocyte adhesion and ICAM-1 expression in brain endothelial cells stimulated with LPS.…”

Psoralea corylifolia protects against testicular torsion/detorsion-induced ischemia/reperfusion injury

“…It has been officially listed in the Pharmacopoeia of the People's Republic of China (Zhao et al, 2005). Several recent reports have revealed that Psoralea corylifolia possesses a variety of biological activities, including anti-oxidative activity, anti-inflammatory effects, antibacterial potential, anticancer activity, and modulation of the immune system (Adhikari et al, 2007;Matsuda et al, 2009;Wang et al, in press). In traditional Chinese medicine, Psoralea corylifolia has been used for the treatment of impotence, premature ejaculation, spermatorrhea, psoriasis, vitiligo, cough, asthma and nephritis (Bensky, 1992;Liu et al, 2004;Qiao et al, 2006).…”

Absolute Stereostructures of Olibanumols A, B, C, H, I, and J from Olibanum, Gum-Resin of Boswellia carterii, and Inhibitors of Nitric Oxide Production in Lipopolysaccharide-Activated Mouse Peritoneal Macrophages

“…45,58) Briefly, peritoneal exudate cells (5ϫ10 5 cells/well) were collected from the peritoneal cavities of male ddY mice and were suspended in 100 ml of RPMI 1640 supplemented with 5% fetal calf serum (FCS), penicillin (100 units/ml) and streptomycin (100 mg/ml), and pre-cultured in 96-well microplates at 37°C in 5% CO 2 in air for 1 h. Nonadherent cells were removed by washing with PBS, and the adherent cells were cultured in 200 ml of fresh medium containing 10 mg/ml LPS and various concentrations of test compound for 20 h. NO production in each well was assessed by measuring the accumulation of nitrite (NO 2 Ϫ ) in the culture medium using Griess reagent. Cytotoxicity was determined by the MTT colorimetric assay, after 20 h incubation with test compounds.…”

“…In the family of NOS, inducible NOS (iNOS) is specifically involved in pathologic aspects with the overproduction of NO and can be expressed in response to proinflammatory agents such as interleukin-1b, tumor necrosis factor-a, and LPS in various cell types including macrophages, endothelial cells, and smooth muscle cells. As a part of our studies to characterize the bioactive components of natural medicines, we have investigated various NO production inhibitors, i.e., higher unsaturated fatty acids, 39) polyacetylenes, [40][41][42] coumarins, 40,42,43) flavonoids, 41,44,45) stilbenes, 46,47) lignans, [48][49][50] sesquiterpenes, [51][52][53][54][55][56][57][58] diterpenes, 59,60) triterpenes, 19,[61][62][63] diarylheptanoids, [64][65][66] cyclic peptides, 63) alkaloids, 67,68) and phenylpropanoids. 50,69,70) As a continuation of these studies on bioactive constituents of natural medicines, the effects of the mono-and triterpene constituents (1-5, 7-13) from the gum-resin of B. carterii on NO production from LPS-activated macrophages were examined, and the results were summarized in Table 3 …”