Bioactive constituents from the leaves of Clinacanthus nutans Lindau

Sakdarat, Santi; Shuyprom, Aussavashai; Pientong, Chamsai; Ekalaksananan, Tipaya; Thongchai, Sasithorn

doi:10.1016/j.bmc.2009.01.059

Cited by 121 publications

(102 citation statements)

References 9 publications

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“…The ovary is packed into two cells and each cell contains two ovules [9] . Phytochemical analysis has shown that C. nutans contains several kinds of clinamide derivatives [10] , alkaloids [11] , chlorophyll [12] , flavonoids [13] , phenolic compounds [14] .…”

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confidence: 99%

Evaluation of Total Phenolic Compound and Cytotoxic Activity of Clinacanthus nutans

Kosai¹,

Sirisidthi²

2016

ijps

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Kosai, et al.: Phenolic and Cytotoxicity of Clinacanthus nutansClinacanthus nutans leaves were extracted in distilled water at 0.5, 1, 3, 5 and 24 h. The plant extracts were determined the highest amount of total phenolic compound and used for evaluating the cytotoxicity test. The median (LC50) and 90% lethal concentration (LC90) against Artemia salina at varied concentrations of this plant extractions as 0, 400, 800, 1600, 3200, 6400 and 12800 µg/ml were evaluated within 24 h exposure. The result revealed that the total phenolic compound measurements in each time extraction were 34.68±3.17, 46.71±9.31, 36.13±7.06, 26.53±8.83 and 30.15±3.09 mg gallic acid equivalent per g of extract, respectively. Due to the highest amount of total phenolic compound, the 1 h aqueous extract of C. nutans leaf expressed the 24 h LC50 and LC90 values against A. salina were 7,151.1 and 12,008.7 µg/ml, respectively.

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confidence: 99%

Evaluation of Total Phenolic Compound and Cytotoxic Activity of Clinacanthus nutans

Kosai¹,

Sirisidthi²

2016

ijps

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“…In addition, supplementation of thylakoid [63] and caffeic acid and chlorogenic acid [24] reduced serum leptin levels in mice. In the current study, Sakdarat et al [64] have been reported that three chlorophyll derivatives (phaeophytins) were extracted from C. nutans leaves, it was expected that thylakoid from plant chloroplasts are useful to elevated the satiety hormone cholecystokinin, which lead to reduce leptin levels [63]. Reduced levels of leptin may suggest enhanced leptin sensitivity [65], which may stimulate fatty acid oxidation.…”

Section: Citationmentioning

confidence: 73%

Methanolic Extract of Clinacanthus nutans Leaves can Alter Adipocyte Cellularity, Inflammation and Acetyl Cholinesterase Activity in Male Obese Mice

Sj¹,

Ebrahimi²,

Y³

et al. 2017

J Obes Weight Loss Ther

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“…The plant has been reported as traditional medicine to prevent and cure diseases such as diarrhea, sores lung watery, diabetes, high blood pressure, weak heart, cardiovascular and cure skin diseases [6]. Research showed that the extracts of F. deltoidea have effects as antioxidant [7,8], antidiabetic [7,9], anti-inflammatory [10,11], antinociceptive [12], antibacterial [13][14][15], anti melanogenic [16], antiherpes simplex virus type 1 (HSV-1), antiproliferation in 3T3-L1 adipocytes [17,18] and antiangiogenic effect [19] to name a few. In our previous research, the result revealed that extracts of F. deltoidea leaves inhibited the growth of breast tumor in mice and inhibit enzyme tyrosine kinase activity [20].…”

Section: Introductionmentioning

confidence: 99%

Methyl 10-Epi-Pheophorbide a From McF-7 Cells Active Layer of the Indonesian Ficus Deltoidea Jack Leaves

Murni

Hanif

Kita

et al. 2017

Int J Pharm Pharm Sci

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Objective: To isolate and elucidate a cytotoxic principle against breast tumor MCF-7 cells of the Indonesian terrestrial plant Ficus deltoidea Jack leaves. Methods:F. deltoidea leaves collected at National Park of mount Gede-Pangrango, Indonesia have been subjected to chemical and biological work. F. deltoidea leaves were extracted with 96% aqueous ethanol (EtOH) and was then partitioned into three layers n-hexane, dichloromethane (CH2Cl2), and n-butanol (n-BuOH). All layers were checked for their activity against breast tumor MCF-7 cells using MTT assay method. A portion of the most active layer was purified using open column chromatography to give fraction that has toxicity against zebra fish embryos. Based on the assay-guided isolation, compound 1 was isolated. The chemical structure of 1 was elucidated using nuclear magnetic resonance (NMR) and high-resolution mass spectrometry (HRMS) data as well as comparing data with literature. Results:The CH2Cl2 layer of F. deltoidea leaves was found to inhibit breast tumor MCF-7 cells with IC50 10 µg/ml which was the most toxic among the layers. A portion of the most active layer was purified using open column chromatography to give 7 fractions. The fraction 5 showed toxicity against zebrafish embryos (LC50 35 µg/ml, 48 hpf). This fraction was purified using high performance liquid chromatography (HPLC) octadecylsilyl (ODS) column with gradient elution 70% aqueous acetonitrile (MeCN) to 100% MeCN (linear gradient) for 40 min with UV detection at 254 nm (tR = 30.99 min) to give compound 1. The chemical structure of 1 was revealed as a chlorin-type compound named methyl 10-epi-pheophorbide A. Conclusion:Methyl 10-epi-pheophorbide A was isolated for the first time from the active fraction of the Indonesian F. deltoidea leaves or tabat barito. The chemical structure including absolute stereochemistry was elucidated using NMR and HRMS data as well as by comparison with the literature values. The 13 C NMR data has been added to complete the previous report.

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Bioactive constituents from the leaves of Clinacanthus nutans Lindau

Cited by 121 publications

References 9 publications

Evaluation of Total Phenolic Compound and Cytotoxic Activity of Clinacanthus nutans

Evaluation of Total Phenolic Compound and Cytotoxic Activity of Clinacanthus nutans

Methanolic Extract of Clinacanthus nutans Leaves can Alter Adipocyte Cellularity, Inflammation and Acetyl Cholinesterase Activity in Male Obese Mice

Methyl 10-Epi-Pheophorbide a From McF-7 Cells Active Layer of the Indonesian Ficus Deltoidea Jack Leaves

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