Bioactive DNA-Peptide Nanotubes Enhance the Differentiation of Neural Stem Cells Into Neurons

Stephanopoulos, Nicholas; Freeman, Ronit; North, Hilary A.; Sur, Shantanu; Jeong, Su Ji; Tantakitti, Faifan; Kessler, John A.; Stupp, Samuel I.

doi:10.1021/nl504079q

Cited by 130 publications

(119 citation statements)

References 65 publications

Supporting

Mentioning

118

Contrasting

Unclassified

Order By: Relevance

“…Furthermore, conjugation of targeting devices (monoclonal antibodies, [15][16][17] aptamers, [18][19][20] or peptides) [21] enables selective deliveryo fD NA vehicles to specific sites. [9,13,22,23] To release the cargo from the carrier, installation of an active devicet hat responds to externals timuli such as light, pH, or redox is required. Several stimuli-responsive devices based on conventional delivery platforms such as liposomes and micelles have been reported.…”

Section: Introductionmentioning

confidence: 99%

DNA Microcapsule for Photo‐Triggered Drug Release Systems

et al. 2017

View full text Add to dashboard Cite

In this study we constructed spherical photo‐responsive microcapsules composed of three photo‐switchable DNA strands. These strands first formed a three‐way junction (TWJ) motif that further self‐assembled to form microspheres through hybridization of the sticky‐end regions of each branch. To serve as the photo‐switch, multiple unmodified azobenzene (Azo) or 2,6‐dimethyl‐4‐(methylthio)azobenzene (SDM‐Azo) were introduced into the sticky‐end regions via a d‐threoninol linker. The DNA capsule structure deformed upon trans‐to‐cis isomerization of Azo or SDM‐Azo induced by specific light irradiation. In addition, photo‐triggered release of encapsulated small molecules from the DNA microcapsule was successfully achieved. Moreover, we demonstrated that photo‐triggered release of doxorubicin caused cytotoxicity to cultured cells. This biocompatible photo‐responsive microcapsule has potential application as a photo‐controlled drug‐release system.

show abstract

Section: Introductionmentioning

confidence: 99%

DNA Microcapsule for Photo‐Triggered Drug Release Systems

et al. 2017

View full text Add to dashboard Cite

show abstract

“…[57] Stupp and coworkers reported the design of a DNA nanotube, which enhanced the differentiation of neural stem cells into neurons (Figure 13C). [234] The DNA nanotubes are self-assembled from five strands, one of which was functionalized by the RGDS sequence. They demonstrated that the co-assembled bioactive DNA/peptide-DNA supramolecular nanostructure promoted the cell adhesion and differentiation.…”

Section: Applications Of Supramolecular Biofunctional Materialsmentioning

confidence: 99%

Supramolecular biofunctional materials

et al. 2017

View full text Add to dashboard Cite

This review discusses supramolecular biofunctional materials, a novel class of biomaterials formed by small molecules that are held together via noncovalent interactions. The complexity of biology and relevant biomedical problems not only inspire, but also demand effective molecular design for functional materials. Supramolecular biofunctional materials offer (almost) unlimited possibilities and opportunities to address challenging biomedical problems. Rational molecular design of supramolecular biofunctional materials exploit powerful and versatile noncovalent interactions, which offer many advantages, such as responsiveness, reversibility, tunability, biomimicry, modularity, predictability, and, most importantly, adaptiveness. In this review, besides elaborating on the merits of supramolecular biofunctional materials (mainly in the form of hydrogels and/or nanoscale assemblies) resulting from noncovalent interactions, we also discuss the advantages of small peptides as a prevalent molecular platform to generate a wide range of supramolecular biofunctional materials for the applications in drug delivery, tissue engineering, immunology, cancer therapy, fluorescent imaging, and stem cell regulation. This review aims to provide a brief synopsis of recent achievements at the intersection of supramolecular chemistry and biomedical science in hope of contributing to the multidisciplinary research on supramolecular biofunctional materials for a wide range of applications. We envision that supramolecular biofunctional materials will contribute to the development of new therapies that will ultimately lead to a paradigm shift for developing next generation biomaterials for medicine.

show abstract

“…, RGDs), which offered a facile pathway to investigate the effects of nanotube architecture and peptide bioactivity in cellular function (Fig. 19a) [327]. DNA origami is another promising approach to create nanoarchitectures and decorate specific functionalities at precise and controlled locations, which not only can help to understand the function of individual moieties, but also can controllably emulate cellular microenvironment.…”

Section: Future Perspectivesmentioning

confidence: 99%

“…(ii, iii) Characterization of generated multiscale PCL nano/micro fiber and in vitro cellular reaction of PCL random multiscale fibers coated with Ch-HA. Reproduced with permission from: (a) [327], (b) [330]. …”

Section: Figurementioning

confidence: 99%

Spatially and temporally controlled hydrogels for tissue engineering

Leijten

Seo

Yue

et al. 2017

Materials Science and Engineering: R: Reports

159

129

View full text Add to dashboard Cite

Summary Recent years have seen tremendous advances in the field of hydrogel-based biomaterials. One of the most prominent revolutions in this field has been the integration of elements or techniques that enable spatial and temporal control over hydrogels’ properties and functions. Here, we critically review the emerging progress of spatiotemporal control over biomaterial properties towards the development of functional engineered tissue constructs. Specifically, we will highlight the main advances in the spatial control of biomaterials, such as surface modification, microfabrication, photo-patterning, and three-dimensional (3D) bioprinting, as well as advances in the temporal control of biomaterials, such as controlled release of molecules, photocleaving of proteins, and controlled hydrogel degradation. We believe that the development and integration of these techniques will drive the engineering of next-generation engineered tissues.

show abstract

Bioactive DNA-Peptide Nanotubes Enhance the Differentiation of Neural Stem Cells Into Neurons

Cited by 130 publications

References 65 publications

DNA Microcapsule for Photo‐Triggered Drug Release Systems

DNA Microcapsule for Photo‐Triggered Drug Release Systems

Supramolecular biofunctional materials

Spatially and temporally controlled hydrogels for tissue engineering

Contact Info

Product

Resources

About