Bioactive Equivalence of Combinatorial Components Identified in Screening of an Herbal Medicine

Liu, Peng; Yang, Hua; Long, Fang; Hao, Haiping; Xu, Xiaojun; Liu, Ying; Shi, Xiaowei; Zhang, Dandan; Zheng, Huirong; Wen, Qian-Ying; Li, Wenwen; Ji, Hui; Jiang, Xijuan; Zhang, Boli; Qi, Lian‐Wen; Li, Ping

doi:10.1007/s11095-013-1283-1

Cited by 82 publications

(54 citation statements)

References 40 publications

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“…Herbal medicines and natural plant-derived agents are important sources for discovering new effective drugs with low toxicity [7]. Imperatorin (IPT, Figure 1) is a natural constituent commonly existing in plants of the Umbelliferae family, such as Peucedanum praeruptorum and Angelica dahurica [8].…”

Section: Introductionmentioning

confidence: 99%

Imperatorin inhibits allergic airway inflammatory reaction and mucin secretion in ovalbumin-induced asthmatic rats

Li¹,

Ding²,

Gao³

et al. 2016

Trop. J. Pharm Res

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Purpose: To study the therapeutic effects of imperatorin (IPT) on allergic asthma induced by ovalbumin in rats. Methods: Asthma was established in rats by injection of ovalbumin (OVA), and IPT (20, 40 and 80 mg/kg) IPT (20, 40 and 80 mg/kg) decreased inflammatory cells including eosinophils (p < 0.01), neutrophils (p < 0.05), lymphocytes (p < 0.01) and macrophages (p < 0.01). In addition, the four inflammatory cytokines {interleukin (IL) -4, IL-6, IL-13 and tumor necrosis factor (TNF)-α} were significantly decreased by treatment with IPT (20, 40 and 80 mg/kg) dose-dependently (p < 0.01).Furthermore, MUC5AC in lung tissues was significantly down-regulated by treatment with IPT (20, 40 and 80 mg/kg, p < 0.01)

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Section: Introductionmentioning

confidence: 99%

Imperatorin inhibits allergic airway inflammatory reaction and mucin secretion in ovalbumin-induced asthmatic rats

Li¹,

Ding²,

Gao³

et al. 2016

Trop. J. Pharm Res

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“…For bioactive equivalence assessment, all the bioassay results were transformed to efficacy value according to the following equation :

\begin{matrix} left {Efficiency}_{drug} & left = & left false[Abs ({Activity}_{drug} - {Activity}_{model}) \\ left / Abs false({Activity}_{control} - {Activity}_{model} false) false] \\ left \times 100 %, \end{matrix}

where Activity drug , Activity model , and Activity control were the measured values of drug group (candidate BECCs, mixture of reference compounds, or original AK treated), model group (LPS‐stimulated RAW 264.7 macrophage inflammation), and control group, respectively, in a given bioassay. Bioactive equivalence was evaluated by calculating 90% CI (where CI is confidence interval) of the ratio between the efficacies of candidate BECCs and AK (two one‐sided t ‐test).…”

Section: Methodsmentioning

confidence: 99%

Anti-inflammatory bioactive equivalence of combinatorial components β-carboline alkaloids identified in Arenaria kansuensis by two-dimensional chromatography and solid-phase extraction coupled with liquid-liquid extraction enrichment technology

et al. 2017

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Bioactive equivalent combinatorial components play a critical role in herbal medicines. However, how to discover and enrich them efficiently is a question for herbal pharmaceuticals researchers. In our work, a novel two-dimensional reversed-phase/hydrophilic interaction high-performance liquid chromatography method was established to perform real-time components trapping and combining for preparation and isolation of coeluting components. Arenaria kansuensis was taken as an example, and solid-phase extraction coupled with liquid-liquid extraction as a simple and efficient method for enriching trace components, reversed phase column coupled with hydrophilic interaction liquid chromatography XAmide column as two-dimensional chromatography technology for isolation and preparation of coeluting constituents, enzyme-linked immune-sorbent assay as bio-guided assay, and anti-inflammatory bioactivity evaluation for bioactive constituents. A combination of 12 β-carboline alkaloids was identified as anti-inflammatory bioactive equivalent combinatorial components from A. kansuensis, which accounts for 1.9% w/w of original A. kansuensis. This work answers the key question of which are real anti-inflammatory components from A. kansuensis and provides a fast and efficient approach for discovering and enriching trace β-carboline alkaloids from herbal medicines for the first time. More importantly, the discovery of bioactive equivalent combinatorial components could improve the quality control of herbal products and inspire a herbal medicine based on combinatorial therapeutics.

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“…Evaluation of HM efficacy is an important prerequisite for the discovery of effective constituents . Therefore, there is an urgent need to establish an innovative strategy for scientific explanation of the effectiveness of HM.…”

Section: Chinmedomics‐guided Mass Spectrometry Used To Advance the Drmentioning

confidence: 99%

Mass spectrometry‐driven drug discovery for development of herbal medicine

Zhang

Sun

Wang

2016

Mass Spectrometry Reviews

141

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Herbal medicine (HM) has made a major contribution to the drug discovery process with regard to identifying products compounds. Currently, more attention has been focused on drug discovery from natural compounds of HM. Despite the rapid advancement of modern analytical techniques, drug discovery is still a difficult and lengthy process. Fortunately, mass spectrometry (MS) can provide us with useful structural information for drug discovery, has been recognized as a sensitive, rapid, and high-throughput technology for advancing drug discovery from HM in the post-genomic era. It is essential to develop an efficient, high-quality, high-throughput screening method integrated with an MS platform for early screening of candidate drug molecules from natural products. We have developed a new chinmedomics strategy reliant on MS that is capable of capturing the candidate molecules, facilitating their identification of novel chemical structures in the early phase; chinmedomics-guided natural product discovery based on MS may provide an effective tool that addresses challenges in early screening of effective constituents of herbs against disease. This critical review covers the use of MS with related techniques and methodologies for natural product discovery, biomarker identification, and determination of mechanisms of action. It also highlights high-throughput chinmedomics screening methods suitable for lead compound discovery illustrated by recent successes.

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Bioactive Equivalence of Combinatorial Components Identified in Screening of an Herbal Medicine

Cited by 82 publications

References 40 publications

Imperatorin inhibits allergic airway inflammatory reaction and mucin secretion in ovalbumin-induced asthmatic rats

Imperatorin inhibits allergic airway inflammatory reaction and mucin secretion in ovalbumin-induced asthmatic rats

Anti-inflammatory bioactive equivalence of combinatorial components β-carboline alkaloids identified in Arenaria kansuensis by two-dimensional chromatography and solid-phase extraction coupled with liquid-liquid extraction enrichment technology

Mass spectrometry‐driven drug discovery for development of herbal medicine

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