Bioactive GlassS53P4 – From a Statistically Suggested Composition to Clinical Success

A comparative analysis of the cytocompatibility, protein adsorption, osteogenic and angiogenic properties of the 45S5- and S53P4-bioactive glass compositions

“…The BG compositions 45S5 and S53P4 make a significant impact in orthopedic applications when it comes to the treatment of bone defects [11,21]. Both BG compositions have been extensively studied regarding their chemical behavior, their processing properties and their biological functions [11,14,22]. However, despite their relevance to the scientific community and frequent clinical utilization, there is no direct comparative data available comparing the biological properties of both BG compositions in one and the same setting.…”

“…However, despite their importance in clinical routine [11,21] and their thorough experimental characterization [11,14,18,20,22], there is a lack of comparative studies analyzing the general biological or-more specifically-the osteogenic and angiogenic properties of both BG compositions subjected to similar experimental conditions. It remains unclear if there are differences in the osteogenic and angiogenic response of osteogenic cells such as bone marrow derived mesenchymal stomal cells (BMSCs) when cultured in the presence of both BGs.…”

“…Although there is a broad number of studies available evaluating the properties of both BGs individually [14,19,22], an actual comparison of the biological effects of the respective BG compositions based on the data available might not necessarily be possible: it is known that differences in the experimental settings, including but not limited to the use of different cell lines, cultivation settings, cultivation periods, evaluation methods, etc., have a significant impact on the obtained results with severe limitations for inter-study comparability [23]. For example, it has been shown that the viability of commonly used 'osteoblast-like' cell lines after exposure to granules made of 45S5-BG differs significantly depending on the cell type: while the MG-63 cell line and BMSCs show similar viability after exposure, the viability of the Saos-2 cell line was significantly reduced when exposed to 45S5-BG in the same concentration [24].…”