Bioactive Indole Alkaloid from Aspergillus amoenus TJ507 That Ameliorates Hepatic Ischemia/Reperfusion Injury

Abstract

Hepatic ischemia/reperfusion injury (IRI) is a major factor contributing to the failure of hepatic resection and liver transplantation. As part of our ongoing investigation into bioactive compounds derived from fungi, we isolated eight indole alkaloids (1–8) from the endophytic fungus Aspergillus amoenus TJ507. Among these alkaloids, one previously undescribed compound, amoenamide D (1), was identified. The planar structure of 1 was elucidated by extensive spectroscopic analysis, including HRESIMS and NMR spectra. The absolute configuration of 1 was elucidated by using electronic circular dichroism calculations. Notably, in the CoCl2-induced hepatocyte damage model, notoamide Q (3) exhibited significant anti-hypoxia injury activity. Furthermore, in a murine hepatic ischemia/reperfusion injury model, treatment with 3 prevents IRI-induced liver damage and hepatocellular apoptosis. Consequently, 3 might serve as a potential lead compound to prevent hepatic ischemia/reperfusion injury.

Effects of Marine Natural Products on Liver Diseases

“…To quantitatively assess the extent of liver injury, the researchers measured serum levels of ALT, AST, and lactate dehydrogenase (LDH), and pretreatment with notoamide Q significantly reduced serum levels of these enzymes when compared to the untreated IRI group. This finding suggests that notoamide Q has the potential to be a lead compound in the development of therapeutic drugs for liver IRI [93]. The chemical structures of compounds 23-32 are shown in Figure 7.…”

Eurochevalierines A–I, Sesquiterpene Alkaloid Hybrids with Anti-Triple Negative Breast Cancer Activity from Penicillium sp. HZ-5

“…After which, only about 11 members of this skeleton have been reported − (Figure S2). In our continuous studies on bioactive molecules from fungi, − nine new sesquiterpene alkaloids, namely eurochevalierines A – I ( 1 – 9 ) were separated from the rice cultures of Penicillium sp. HZ-5, acquired from Hypericum wilsonii N. Robson (Figure ).…”