Bioactive injectable aggregates with nanofibrous microspheres and human dental pulp stem cells: A translational strategy in dental endodontics

Garzón, Ingrid; Martín-Piedra, Miguel Ángel; Carriel, Víctor; Alaminos, Miguel; Liu, X; D’Souza, Rena N.

doi:10.1002/term.2397

Cited by 25 publications

(16 citation statements)

References 33 publications

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“…In the present work, novel models of bioengineered skin generated by using four MSC types were described. In agreement with previous reports, suggesting that heterotypical bioengineered tissues have low levels of differentiation while kept in vitro (Garzon et al, 2013;Garzon et al, 2009a), in vitro tissues had very few cell layers on top of the dermis substitute -although the number of cell layers tended to increase with time -and a complete absence of differentiation markers, such as rete ridges and chorial papillae or keratinisation.…”

Section: Discussionsupporting

confidence: 92%

“…An interesting phenomenon was that BMSCs showed slight CK10 expression in vitro, but this expression was initially negative in vivo and became positive at 30 d. This finding may be explained as an adaptive process of the cells, which could partially lose their original orientation in vivo and, thus, minimise their expression profile until the epithelium became fully differentiated. These results, related to the in vitro differentiation potential of WJSCs and BMSCs, were in agreement with previous work demonstrating their keratinocytic differentiation capability (Garzon et al, 2013) and with the fact that ADSCs and DPSCs have not yet been differentiated into skin keratinocytes -although reports have suggested that these cell types may have epithelial differentiation capability in the presence of inductive medium (Baer et al, 2013;Dogan et al, 2015). In the present study, both WJSCs and BMSCs were able to develop a larger number of cell layers at the in vitro stage, which coincided with the early cytokeratin expression.…”

Section: Discussionsupporting

confidence: 92%

“…In this regard, preliminary reports support the epithelial differentiation capability of ADSCs (Baer et al, 2013) and human tooth-derived stem cells (Dogan et al, 2015). In turn, WJSCs are efficiently differentiated into cornea epithelial cells (Garzon et al, 2014) and human skin as well as oral mucosa keratinocytes (Garzon et al, 2013). BMSCs, the most characterised MSC source, can differentiate into several types of epithelial cells, including skin keratinocytes (Kokubun et al, 2016;Mahdavishahri et al, 2012;Mendez et al, 2014;Wang et al, 2014;Zhang et al, 2012).…”

Section: Introductionmentioning

confidence: 95%

“…To generate primary cultures of ADSCs, WJSCs and BMSCs, small biopsies were obtained from adipose tissue, umbilical cord and bone marrow, as previously described (Alfonso-Rodriguez et al, 2015;Cejka et al, 2016;Dori et al, 2017). To generate DPSC cultures, extracted sound third molars were obtained (Garzon et al, 2017). In all cases, tissues were obtained from healthy donors subjected to surgical procedures not related to the present study.…”

Section: Generation Of Msc Primary Cell Culturesmentioning

confidence: 99%

“…However, the four main sources of MSCs in humans are adipose-tissue-derived stem cells (ADSCs) (Zuk et al, 2002), dental pulp stem cells (DPSCs) (Gronthos et al, 2000), umbilical cord Wharton's jelly stem cells (WJSCs) (Wang et al, 2004) and bone marrow stem cells (BMSCs) (Friedenstein et al, 1976;Pittenger et al, 1999). In addition to adipocyte, chondrocyte and osteoblast differentiation potential, MSCs have epithelial differentiation capability and heterotypical models of artificial tissues could be generated using MSCs as alternative cell sources (Garzon et al, 2013). In this regard, preliminary reports support the epithelial differentiation capability of ADSCs (Baer et al, 2013) and human tooth-derived stem cells (Dogan et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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Effective use of mesenchymal stem cells in human skin substitutes generated by tissue engineering

Martín-Piedra¹,

Alfonso²,

Zapater³

et al. 2019

eCM

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Mesenchymal stem cells (MSCs) can differentiate toward epithelial cells and may be used as an alternative source for generation of heterotypical artificial human skin substitutes, thus, enhancing their development and translation potential to the clinic. The present study aimed at comparing four types of heterotypical human bioengineered skin generated using MSCs as an alternative epithelial cell source. Adipose-tissue-derived stem cells (ADSCs), dental pulp stem cells (DPSCs), Wharton's jelly stem cells (WJSCs) and bone marrow stem cells (BMSCs) were used for epidermal regeneration on top of dermal skin substitutes. Heterotypic human skin substitutes were evaluated before and after implantation in immune-deficient athymic mice for 30 d. Histological and genetic studies were performed to evaluate extracellular matrix synthesis, epidermal differentiation and human leukocyte antigen (HLA) molecule expression. The four cell types differentiated into keratinocytes, as shown by the expression of cytokeratin 10 and filaggrin 30 d post-grafting; also, they induced dermal fibroblasts responsible for the synthesis of extracellular fibrillar and non-fibrillar components, in a similar way among each other. WJSCs and BMSCs showed higher expression of cytokeratin 10 and filaggrin, suggesting these cells were more prone to epidermal regeneration. The absence of HLA molecules, even when the epithelial layer was differentiated, supports the future clinical use of these substitutes-especially ADSCs, DPSCs and WJSCs-with low rejection risk. MSCs allowed the generation of bioengineered human skin substitutes with potential clinical usefulness. According to their epidermal differentiation potential and lack of HLA antigens, WJSCs should preferentially be used.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 95%

Section: Generation Of Msc Primary Cell Culturesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Effective use of mesenchymal stem cells in human skin substitutes generated by tissue engineering

Martín-Piedra¹,

Alfonso²,

Zapater³

et al. 2019

eCM

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Identification of histological threshold concepts in health sciences curricula: Students' perception

Martín-Piedra

Saavedra‐Casado

Santisteban‐Espejo

et al. 2022

Anatomical Sciences Ed

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Students' metacognitive skills and perceptions are considered important variables for high-quality learning. In this study, students' perceptions were used to identify histological threshold concepts (integrative, irreversible, transformative, and troublesome) in three health sciences curricula. A specific questionnaire was developed and validated to characterize students' perceptions of histological threshold concepts. A sample of 410 undergraduate students enrolled in the dentistry, medicine, and pharmacy degree programs participated in the study. Concepts assessed in the study were clustered to ten categories (factors) by exploratory and confirmatory factor analysis. Concepts linked to tissue organization and tissue functional states received the highest scores from students in all degree programs, suggesting that the process of learning histology requires the integration of both static concepts related to the constituent elements of tissues and dynamic concepts such as stem cells as a tissue renewal substrate, or the euplasic, proplasic and retroplasic states of tissues. The complexity of integrating static and dynamic concepts may pose a challenging barrier to the comprehension of histology. In addition, several differences were detected among the students in different degree programs. Dentistry students more often perceived morphostructural concepts as threshold concepts, whereas medical students highlighted concepts related to two-dimensional microscopic identification. Lastly, pharmacy students identified concepts related to tissue general activity as critical for the comprehension and learning of histology. The identification of threshold concepts through students' perceptions is potentially useful to improve the teaching and learning process in health sciences curricula.

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Parathyroid hormone enhances the osteo/odontogenic differentiation of dental pulp stem cells via ERK and P38 MAPK pathways

Jing

Wang

et al. 2019

Journal Cellular Physiology

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Objective: Parathyroid hormone (PTH) is a main systemic mediator of calcium and phosphate homeostasis in the bone. Dental pulp stem cells (DPSCs) have been extensively studied in the regeneration of bone and tooth tissues. This paper aims to uncover the influences of PTH on the proliferative ability and osteo/odontogenic differentiation of DPSCs, as well as the underlying mechanisms. Materials and Methods: The optimal concentration of PTH on DPSCs was determined by alkaline phosphatase (ALP) activity assay, ALP staining and western blot analysis. Proliferative ability and cell cycle distribution of DPSCs were analyzed by Cell counting kit-8, 5-ethynyl-20-deoxyuridine assay, and flow cytometry. Osteo/ odontogenic capacity of DPSCs was evaluated and finally, the involvement of mitogen-activated protein kinase (MAPK) pathway was assessed.Results: Purified DPSCs were obtained by enzymatic digestion, which presented a typical fibroblast-like morphology. 10 −9 mol/L PTH was concerned as the optimal concentration for DPSCs induction. 10 −9 mol/L PTH treatment did not change the proliferative rate of DPSCs (p > .05). Relative expressions of DSPP/DSPP, RUNX2/ RUNX2, OSX/OSX, and ALP/ALP were upregulated in PTH-treated DPSCs relative to control group. Particularly, their mRNA/protein levels at Day 7 were markedly higher relative to those at Day 3 (p < .05 or p < .01). Mineralized nodules were formed after PTH induction, and calcium content increased by cetylpyridinium chloride quantitative analysis. Mechanistically, the protein levels of p-ERK and p-P38 significantly increased after PTH treatment, and the inhibitors targeting MAPK were identified that weakened the effects of PTH on the committed differentiation of DPSCs.Conclusions: PTH enhances the osteo/odontogenic differentiation capacity of DPSCs via ERK and P38 signaling pathways. K E Y W O R D Sdental pulp stem cells, differentiation, mitogen-activated protein kinase, parathyroid hormone *Xingyun Ge, Zehan Li, and Shuanglin Jing contributed equally to this study.

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Bioactive injectable aggregates with nanofibrous microspheres and human dental pulp stem cells: A translational strategy in dental endodontics

Cited by 25 publications

References 33 publications

Effective use of mesenchymal stem cells in human skin substitutes generated by tissue engineering

Effective use of mesenchymal stem cells in human skin substitutes generated by tissue engineering

Identification of histological threshold concepts in health sciences curricula: Students' perception

Parathyroid hormone enhances the osteo/odontogenic differentiation of dental pulp stem cells via ERK and P38 MAPK pathways

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